Optimal thawing of cryopreserved peripheral blood mononuclear cells for use in high-throughput human immune monitoring studies

Cells

Volumn 1, Issue 3, 2012, Pages 313-324

  Ramachandran, Hari ^a   Laux, Jessica ^a   Moldovan, Ioana ^a   Caspell, Richard ^a   Lehmann, Paul V ^a   Subbramanian, Ramu A ^a  

^a Cellular Technology Limited   (United States)

Author keywords

Cryopreservation; DMSO; ELISPOT; PBMC; T cells

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN;

ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CRYOPRESERVATION; CYTOMEGALOVIRUS; ENVIRONMENT; ENZYME LINKED IMMUNOSPOT ASSAY; EPSTEIN BARR VIRUS; EXPOSURE; HUMAN; HUMAN CELL; INFLUENZA VIRUS; MONITORING; PERIPHERAL BLOOD MONONUCLEAR CELL; RISK; T LYMPHOCYTE; TEMPERATURE; THAWING; WARMING; WILCOXON SIGNED RANKS TEST;

EID: 84870603380     PISSN: None     EISSN: 20734409     Source Type: Journal    
DOI: 10.3390/cells1030313     Document Type: Article

References (35)

1. Kreher, C.R.; Dittrich, M.T.; Guerkov, R.; Boehm, B.O.; Tary-Lehmann, M. Cd4+ and cd8+ cells in cryopreserved human pbmc maintain full functionality in cytokine elispot assays. J. Immunol. Methods 2003, 278, 79-93.
2. Currier, J.R.; deSouza, M.; Chanbancherd, P.; Bernstein, W.; Birx, D.L.; Cox, J.H. Comprehensive screening for human immunodeficiency virus type 1 subtype-specific cd8 cytotoxic t lymphocytes and definition of degenerate epitopes restricted by hla-a0207 and-c(w)0304 alleles. J. Virol. 2002, 76, 4971-4986.
3. Peters, B.S.; Jaoko, W.; Vardas, E.; Panayotakopoulos, G.; Fast, P.; Schmidt, C.; Gilmour, J.; Bogoshi, M.; Omosa-Manyonyi, G.; Dally, L.; et al. Studies of a prophylactic hiv-1 vaccine candidate based on modified vaccinia virus ankara (mva) with and without DNA priming: Effects of dosage and route on safety and immunogenicity. Vaccine 2007, 25, 2120-2127.
4. Basha, S.; Hazenfeld, S.; Brady, R.C.; Subbramanian, R.A. Comparison of antibody and t-cell responses elicited by licensed inactivated-and live-attenuated influenza vaccines against h3n2 hemagglutinin. Hum. Immunol. 2011, 72, 463-469.
5. Garcia, F.; Bernaldo de Quiros, J.C.; Gomez, C.E.; Perdiguero, B.; Najera, J.L.; Jimenez, V.; Garcia-Arriaza, J.; Guardo, A.C.; Perez, I.; Diaz-Brito, V.; et al. Safety and immunogenicity of a modified pox vector-based hiv/aids vaccine candidate expressing env, gag, pol and nef proteins of hiv-1 subtype b (mva-b) in healthy hiv-1-uninfected volunteers: A phase i clinical trial (risvac02). Vaccine 2011, 29, 8309-8316.
6. Yang, F.F.; Tu, Z.Q.; Fang, Y.M.; Li, Y.; Peng, Y.; Dong, T.; Wang, C.; Lin, S.X.; Zhan, N.Y.; Ma, Z.M.; et al. Monitoring of peptide-specific and interferon-gamma-productive t cells in patients with active and convalescent tuberculosis using elispot. Clin. Vaccine Immunol. 2012, doi:10.1128/CVI.05544-11.
7. Axelsson, S.; Faresjo, M.; Hedman, M.; Ludvigsson, J.; Casas, R. Cryopreserved peripheral blood mononuclear cells are suitable for the assessment of immunological markers in type 1 diabetic children. Cryobiology 2008, 57, 201-208.
8. Weinberg, A.; Song, L.Y.; Wilkening, C.L.; Fenton, T.; Hural, J.; Louzao, R.; Ferrari, G.; Etter, P.E.; Berrong, M.; Canniff, J.D.; et al. Optimization of storage and shipment of cryopreserved peripheral blood mononuclear cells from hiv-infected and uninfected individuals for elispot assays. J. Immunol. Methods 2010, 363, 42-50.
9. Zhang, W.; Caspell, R.; Karulin, A.Y.; Ahmad, M.; Haicheur, N.; Abdelsalam, A.; Johannesen, K.; Vignard, V.; Dudzik, P.; Georgakopoulou, K.; et al. Elispot assays provide reproducible results among different laboratories for t-cell immune monitoring Even in hands of elispot-inexperienced investigators. J. Immunotoxicol. 2009, 6, 227-234.
10. Areman, E.M.; Simonis, T.B.; Carter, C.S.; Read, E.J.; Klein, H.G. Bulk cryopreservation of lymphocytes in glycerol. Transfusion 1988, 28, 151-156.
11. Yokoyama, W.M. Cryopreservation of Cells. In Current Protocols in Immunology, Coligan, J.E., Kruisbeek, A.M., Margulies, D.H., Shevach, E.M., Strober, W., Eds.; Wiley: New York, NY, USA, 1997; pp. A.3.G.1-A.3.G.3.
12. Birkeland, S.A. The influence of different freezing procedures and different cryoprotective agents on the immunological capacity of frozen-stored lymphocytes. Cryobiology 1976, 13, 442-447.
13. Wang, S.Y.; Hsu, M.L.; Tzeng, C.H.; Hsu, H.C.; Ho, C.K. The influence of cryopreservation on cytokine production by human t lymphocytes. Cryobiology 1998, 37, 22-29.
14. Jeurink, P.V.; Vissers, Y.M.; Rappard, B.; Savelkoul, H.F. T cell responses in fresh and cryopreserved peripheral blood mononuclear cells: Kinetics of cell viability, cellular subsets, proliferation, and cytokine production. Cryobiology 2008, 57, 91-103.
15. Posavad, C.M.; Magaret, A.S.; Zhao, L.; Mueller, D.E.; Wald, A.; Corey, L. Development of an interferon-gamma elispot assay to detect human t cell responses to hsv-2. Vaccine 2011, 29, 7058-7066.
16. Cox, J.H.; Ferrari, G.; Kalams, S.A.; Lopaczynski, W.; Oden, N.; D'Souza, M.P. Results of an elispot proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials. AIDS Res. Hum. Retroviruses 2005, 21, 68-81.
17. Weinberg, A.; Song, L.Y.; Wilkening, C.; Sevin, A.; Blais, B.; Louzao, R.; Stein, D.; Defechereux, P.; Durand, D.; Riedel, E.; et al. Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic t-cell characterization. Clin. Vaccine Immunol. 2009, 16, 1176-1186.
18. Gill, D.K.; Huang, Y.; Levine, G.L.; Sambor, A.; Carter, D.K.; Sato, A.; Kopycinski, J.; Hayes, P.; Hahn, B.; Birungi, J.; et al. Equivalence of elispot assays demonstrated between major hiv network laboratories. PLoS One 2010, 5, doi:10.1371/journal.pone.0014330.
19. Almeida, C.A.; Roberts, S.G.; Laird, R.; McKinnon, E.; Ahmed, I.; Pfafferott, K.; Turley, J.; Keane, N.M.; Lucas, A.; Rushton, B.; et al. Automation of the elispot assay for high-throughput detection of antigen-specific t-cell responses. J. Immunol. Methods 2009, 344, 1-5.
20. Trubiani, O.; Salvolini, E.; Staffolani, R.; di Primio, R.; Mazzanti, L. Dmso modifies structural and functional properties of rpmi-8402 cells by promoting programmed cell death. Int. J. Immunopathol. Pharmacol. 2003, 16, 253-259.
21. Trubiani, O.; Ciancarelli, M.; Rapino, M.; Di Primio, R. Dimethyl sulfoxide induces programmed cell death and reversible g1 arrest in the cell cycle of human lymphoid pre-t cell line. Immunol. Lett. 1996, 50, 51-57.
22. Suneetha, P.V.; Schlaphoff, V.; Wang, C.; Stegmann, K.A.; Fytili, P.; Sarin, S.K.; Manns, M.P.; Cornberg, M.; Wedemeyer, H. Effect of peptide pools on effector functions of antigen-specific cd8+ t cells. J. Immunol. Methods 2009, 342, 33-48.
23. Baust, J.M.; Van Buskirk, R.; Baust, J.G. Modulation of the cryopreservation cap: Elevated survival with reduced dimethyl sulfoxide concentration. Cryobiology 2002, 45, 97-108.
24. Deng, Y.; Jing, Y.; Campbell, A.E.; Gravenstein, S. Age-related impaired type 1 t cell responses to influenza: Reduced activation ex vivo, decreased expansion in ctl culture in vitro, and blunted response to influenza vaccination in vivo in the elderly. J. Immunol. 2004, 172, 3437-3446.
25. Mahnke, Y.D.; Saqr, A.; Hazenfeld, S.; Brady, R.C.; Roederer, M.; Subbramanian, R.A. Age-related changes in durability and function of vaccine-elicited influenza-specific cd4(+) t-cell responses. Vaccine 2011, 29, 8606-8614.
26. Sasaki, S.; He, X.S.; Holmes, T.H.; Dekker, C.L.; Kemble, G.W.; Arvin, A.M.; Greenberg, H.B. Influence of prior influenza vaccination on antibody and b-cell responses. PLoS One 2008, 3, doi:10.1371/journal.pone.0002975.
27. Russell, N.D.; Hudgens, M.G.; Ha, R.; Havenar-Daughton, C.; McElrath, M.J. Moving to human immunodeficiency virus type 1 vaccine efficacy trials: Defining t cell responses as potential correlates of immunity. J. Infect. Dis. 2003, 187, 226-242.
28. Ghanekar, S.A.; Bhatia, S.; Ruitenberg, J.J.; DeLa Rosa, C.; Disis, M.L.; Maino, V.C.; Maecker, H.T.; Waters, C.A. Phenotype and in vitro function of mature mddc generated from cryopreserved pbmc of cancer patients are equivalent to those from healthy donors. J. Immune Based Ther. Vaccines 2007, 5, doi:10.1186/1476-8518-5-7.
29. Lehmann, P.V.; Zhang, W. Unique strengths of elispot for t cell diagnostics. Methods Mol. Biol. 2012, 792, 3-23.
30. Addo, M.M.; Yu, X.G.; Rathod, A.; Cohen, D.; Eldridge, R.L.; Strick, D.; Johnston, M.N.; Corcoran, C.; Wurcel, A.G.; Fitzpatrick, C.A.; et al. Comprehensive epitope analysis of human immunodeficiency virus type 1 (hiv-1)-specific t-cell responses directed against the entire expressed hiv-1 genome demonstrate broadly directed responses, but no correlation to viral load. J. Virol. 2003, 77, 2081-2092.
31. Moss, R.B.; Diveley, J.; Jensen, F.C.; Gouveia, E.; Savary, J.; Carlo, D.J. Hiv-specific cd4(+) and cd8(+) immune responses are generated with a gp120-depleted, whole-killed hiv-1 immunogen with cpg immunostimulatory sequences of DNA. J. Interferon. Cytokine Res. 2000, 20, 1131-1137.
32. Subbramanian, R.A.; Basha, S.; Shata, M.T.; Brady, R.C.; Bernstein, D.I. Pandemic and seasonal h1n1 influenza hemagglutinin-specific t cell responses elicited by seasonal influenza vaccination. Vaccine 2010, 28, 8258-8267.
33. Kloverpris, H.; Fomsgaard, A.; Handley, A.; Ackland, J.; Sullivan, M.; Goulder, P. Dimethyl sulfoxide (dmso) exposure to human peripheral blood mononuclear cells (pbmcs) abolish t cell responses only in high concentrations and following coincubation for more than two hours. J. Immunol. Methods 2010, 356, 70-78.
34. Kiecker, F.; Streitz, M.; Ay, B.; Cherepnev, G.; Volk, H.D.; Volkmer-Engert, R.; Kern, F. Analysis of antigen-specific t-cell responses with synthetic peptides What kind of peptide for which purpose? Hum. Immunol. 2004, 65, 523-536.
35. Lehmann, P.V. Image analysis and data management of elispot assay results. Methods Mol. Biol. 2005, 302, 117-132.