Cryopreserved peripheral blood mononuclear cells are suitable for the assessment of immunological markers in type 1 diabetic children

Cryobiology

Volumn 57, Issue 3, 2008, Pages 201-208

  Axelsson, Stina ^a   Faresjö, Maria ^a   Hedman, Maria ^a   Ludvigsson, Johnny ^a   Casas, Rosaura ^a  

^a Diabetes Research Centre   (Sweden)

Author keywords

BMC; Chemokine; Cryopreservation; Cytokine; Multiplex fluorochrome technique; Real time RT PCR; Type 1 diabetes

Indexed keywords

CELL MARKER; CHEMOKINE; CRYOPROTECTIVE AGENT; CYTOKINE; FLUOROCHROME; GAMMA INTERFERON; GAMMA INTERFERON INDUCIBLE PROTEIN 10; GLUTAMATE DECARBOXYLASE 65; INTERLEUKIN 10; INTERLEUKIN 12; INTERLEUKIN 13; INTERLEUKIN 17; INTERLEUKIN 5; INTERLEUKIN 6; MACROPHAGE INFLAMMATORY PROTEIN 1ALPHA; MACROPHAGE INFLAMMATORY PROTEIN 1BETA; MESSENGER RNA; MONOCYTE CHEMOTACTIC PROTEIN 1; PHYTOHEMAGGLUTININ; RANTES; TRANSCRIPTION FACTOR FOXP3; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA;

ADOLESCENT; ADULT; ARTICLE; CHILD; CHILDHOOD DISEASE; CLINICAL ARTICLE; CRYOPRESERVATION; FEMALE; HUMAN; HUMAN CELL; IMMUNE RESPONSE; INSULIN DEPENDENT DIABETES MELLITUS; MALE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REGULATORY T LYMPHOCYTE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADOLESCENT; CELLS, CULTURED; CHEMOKINES; CHILD; CRYOPRESERVATION; CYTOKINES; DIABETES MELLITUS, TYPE 1; FEMALE; FORKHEAD TRANSCRIPTION FACTORS; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; RNA, MESSENGER; TRANSFORMING GROWTH FACTOR BETA;

EID: 56749180780     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2008.08.001     Document Type: Article

References (22)

1. Atkinson M.A., and Maclaren N.K. The pathogenesis of insulin-dependent diabetes mellitus. N. Engl. J. Med. 331 (1994) 1428-1436
2. Chen W., and Wahl S.M. TGF-beta: the missing link in CD4+CD25+ regulatory T cell-mediated immunosuppression. Cytokine Growth Factor Rev. 14 (2003) 85-89
3. Costantini A., Mancini S., Giuliodoro S., Butini L., Regnery C.M., Silvestri G., and Montroni M. Effects of cryopreservation on lymphocyte immunophenotype and function. J. Immunol. Methods 278 (2003) 145-155
4. de Jager W., te Velthuis H., Prakken B.J., Kuis W., and Rijkers G.T. Simultaneous detection of 15 human cytokines in a single sample of stimulated peripheral blood mononuclear cells. Clin. Diagn. Lab. Immunol. 10 (2003) 133-139
5. Hansen A., Reiter K., Dorner T., and Pruss A. Cryopreserved human B cells as an alternative source for single cell mRNA analysis. Cell Tissue Bank. 6 (2005) 299-308
6. Karlsson Faresjo M.G., Ernerudh J., and Ludvigsson J. Cytokine profile in children during the first 3 months after the diagnosis of type 1 diabetes. Scand. J. Immunol. 59 (2004) 517-526
7. Kreher C.R., Dittrich M.T., Guerkov R., Boehm B.O., and Tary-Lehmann M. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J. Immunol. Methods 278 (2003) 79-93
8. Kvarnstrom M., Jenmalm M.C., and Ekerfelt C. Effect of cryopreservation on expression of Th1 and Th2 cytokines in blood mononuclear cells from patients with different cytokine profiles, analysed with three common assays: an overall decrease of interleukin-4. Cryobiology 49 (2004) 157-168
9. Lagrelius M., Jones P., Franck K., and Gaines H. Cytokine detection by multiplex technology useful for assessing antigen specific cytokine profiles and kinetics in whole blood cultured up to seven days. Cytokine 33 (2006) 156-165
10. Leslie D., Lipsky P., and Notkins A.L. Autoantibodies as predictors of disease. J. Clin. Invest. 108 (2001) 1417-1422
11. Mosmann T.R., and Sad S. The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol. Today 17 (1996) 138-146
12. Romagnani S. Understanding the role of Th1/Th2 cells in infection. Trends Microbiol. 4 (1996) 470-473
13. Sakaguchi S., Setoguchi R., Yagi H., and Nomura T. Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in self-tolerance and autoimmune disease. Curr. Top. Microbiol. Immunol. 305 (2006) 51-66
14. Sobota V., Bubenik J., Indrova M., Vlk V., and Jakoubkova J. Use of cryopreserved lymphocytes for assessment of the immunological effects of interferon therapy in renal cell carcinoma patients. J. Immunol. Methods 203 (1997) 1-10
15. Stechova K., Bohmova K., Vrabelova Z., Sepa A., Stadlerova G., Zacharovova K., and Faresjo M. High T-helper-1 cytokines but low T-helper-3 cytokines, inflammatory cytokines and chemokines in children with high risk of developing type 1 diabetes. Diabetes Metab. Res. Rev. 23 (2007) 462-471
16. Szabo S.J., Kim S.T., Costa G.L., Zhang X., Fathman C.G., and Glimcher L.H. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 100 (2000) 655-669
17. Ting C.N., Olson M.C., Barton K.P., and Leiden J.M. Transcription factor GATA-3 is required for development of the T-cell lineage. Nature 384 (1996) 474-478
18. Wang S.Y., Hsu M.L., Tzeng C.H., Hsu H.C., and Ho C.K. The influence of cryopreservation on cytokine production by human T lymphocytes. Cryobiology 37 (1998) 22-29
19. Venkataraman M. Effects of cryopreservation of immune responses. XI. Heightened secretion of tumor necrosis factor-alpha by frozen human peripheral blood mononuclear cells. Cryobiology 34 (1997) 276-283
20. Venkataraman M. Effects of cryopreservation on immune responses. VIII. Enhanced secretion of interferon-gamma by frozen human peripheral blood mononuclear cells. Cryobiology 32 (1995) 528-534
21. Venkataraman M. Effects of cryopreservation on immune responses: VII. Freezing induced enhancement of IL-6 production in human peripheral blood mononuclear cells. Cryobiology 31 (1994) 468-477
22. Venkataraman M., and Westerman M.P. Cryopreservation enhances interleukin-1 production in human mononuclear cells. Cryobiology 27 (1990) 137-142