Fungal His-Tagged Nitrilase from Gibberella intermedia: Gene Cloning, Heterologous Expression and Biochemical Properties

PLoS ONE

Volumn 7, Issue 11, 2012, Pages

  Gong, Jin Song ^a   Li, Heng ^a   Zhu, Xiao Yan ^a   Lu, Zhen Ming ^a   Wu, Yan ^a   Shi, Jing Song ^a   Xu, Zheng Hong ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; CYSTEINE; GLUTAMINE; LYSINE; NICOTINAMIDE; NICOTINIC ACID; NITRILASE; NITRILE; POLYHISTIDINE TAG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE EXPRESSION; GIBBERELLA; GIBBERELLA INTERMEDIA; HETEROLOGOUS EXPRESSION; HYDROLYSIS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; PH; PROTEIN PURIFICATION; RECOMBINANT GENE; RESIDUE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; TEMPERATURE SENSITIVITY; THEORETICAL STUDY;

AMINO ACID SEQUENCE; AMINOHYDROLASES; CLONING, MOLECULAR; GENE EXPRESSION; GIBBERELLA; HISTIDINE; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; NITRILES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GIBBERELLA INTERMEDIA;

EID: 84870597374     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0050622     Document Type: Article

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