Improved vectors for selection of transgenic caenorhabditis elegans

Methods in Molecular Biology

Volumn 940, Issue , 2013, Pages 87-102

  Ferguson, Annabel A ^a   Cai, Liquan ^a   Kashyap, Luv ^a   Fisher, Alfred L ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Biotechnology; C. elegans; Cre recombinase; Microparticle bombardment; Plasmid; Recombination; Transgenic animals; unc 119

Indexed keywords

AMPICILLIN; CAENORHABDITIS ELEGANS PROTEIN; ENDODEOXYRIBONUCLEASE SCAI; KANAMYCIN; NERVE PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNC 119 PROTEIN, C ELEGANS; UNC-119 PROTEIN, C ELEGANS;

ANIMAL; ANTIBIOTIC RESISTANCE; ARTICLE; CAENORHABDITIS ELEGANS; CYTOLOGY; DRUG EFFECT; ELECTROPORATION; ESCHERICHIA COLI; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; METHODOLOGY; PLASMID; POLYMERASE CHAIN REACTION; TRANSGENIC ANIMAL;

AMPICILLIN; ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DRUG RESISTANCE, BACTERIAL; ELECTROPORATION; ESCHERICHIA COLI; GENETIC ENGINEERING; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; KANAMYCIN; NERVE TISSUE PROTEINS; PLASMIDS; POLYMERASE CHAIN REACTION; TRANSFORMATION, GENETIC;

ANIMALIA; CAENORHABDITIS ELEGANS; ESCHERICHIA COLI;

EID: 84870546305     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-110-3_8     Document Type: Article

References (14)

1. Mello C, Fire A (1995) DNA transformation. Methods Cell Biol 48:451-482
2. Evans TC (2006) Transformation and microinjection. In: WormBook (ed) The C. elegans research community, WormBook. doi/10.1895/wormbook.1.108.1 .
3. Praitis V et al (2001) Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157:1217-1226
4. Maduro M, Pilgrim D (1995) Identi fication and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system. Genetics 141:977-988
5. Ferguson AA, Fisher AL (2009) Retro fitting ampicillin resistant vectors by recombination for use in generating C. elegans transgenic animals by bombardment. Plasmid 62:140-145
6. Zhang Y, Nash L, Fisher AL (2008) A simpli fied, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering. BMC Dev Biol 8:119
7. Fisher AL et al (2008) The Caenorhabditis elegans K10C2.4 gene encodes a member of the fumarylacetoacetate hydrolase family: a Caenorhabditis elegans model of type I tyrosinemia. J Biol Chem 283:9127-9135
8. Ferguson AA, Springer MG, Fisher AL (2010) skn-1-Dependent and -independent regulation of aip-1 expression following metabolic stress in Caenorhabditis elegans. Mol Cell Biol 30:2651-2667
9. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640-6645
10. Warming S et al (2005) Simple and highly ef ficient BAC recombineering using galK selection. Nucleic Acids Res 33:e36
11. Thomason LC et al (2007) Multicopy plasmid modi fication with phage lambda Red recombineering. Plasmid 58:148-158
12. Tursun B et al (2009) A toolkit and robust pipeline for the generation of fosmid-based reporter genes in C. elegans. PLoS One 4:e4625
13. Sarov M et al (2006) A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. Nat Methods 3:839-844
14. Dolphin CT, Hope IA (2006) Caenorhabditis elegans reporter fusion genes generated by seamless modi fication of large genomic DNA clones. Nucleic Acids Res 34:e72