Transient expression of artificial microRNAs targeting Grapevine fanleaf virus and evidence for RNA silencing in grapevine somatic embryos

Transgenic Research

Volumn 21, Issue 6, 2012, Pages 1319-1327

  Jelly, Noémie S ^a,b   Schellenbaum, Paul ^a   Walter, Bernard ^a   Maillot, Pascale ^a  

^a UNIVERSITÉ DE HAUTE ALSACE   (France)

^b UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Agrobacterium tumefaciens; Fanleaf degeneration; Transient transformation; Vitis vinifera

Indexed keywords

BETA GLUCURONIDASE; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; MICRORNA; PLANT RNA; VIRUS RNA;

ARABIDOPSIS; ARTICLE; GENE TRANSFER; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; NEPOVIRUS; PHYSIOLOGY; PRENATAL DEVELOPMENT; RHIZOBIUM RADIOBACTER; RNA INTERFERENCE; VITIS;

AGROBACTERIUM TUMEFACIENS; ARABIDOPSIS; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; MICRORNAS; NEPOVIRUS; RNA INTERFERENCE; RNA, PLANT; RNA, VIRAL; VITIS;

AGROBACTERIUM TUMEFACIENS; ARABIDOPSIS THALIANA; FIORIA; GRAPEVINE FANLEAF VIRUS; VITACEAE; VITIS; VITIS VINIFERA;

EID: 84870360746     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-012-9611-5     Document Type: Article

References (51)

1. Ai T, Zhang L, Gao Z, Zhu CX, Guo X (2011) Highly efficient virus resistance mediated by artificial microRNAs that target the suppressor of PVX and PVY in plants. Plant Biol (Stuttg) 13: 304-316.
2. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116: 281-297.
3. Brown DJF, Robertson WM, Trudgill DL (1995) Transmission of viruses by plant nematodes. Annu Rev Phytopathol 33: 223-249.
4. Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ (2005) Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33: e179.
5. Duan CG, Wang CH, Fang RX, Guo HS (2008) Artificial MicroRNAs highly accessible to targets confer efficient virus resistance in plants. J Virol 82: 11084-11095.
6. Dupuis L (2010) Study of the interactions between Arabis mosaic virus and its host plants. PhD Thesis, Ruprecht-Karls-Universität Heidelberg & Université de Strasbourg.
7. Fahim M, Millar AA, Wood CC, Larkin PJ (2011) Resistance to Wheat streak mosaic virus generated by expression of an artificial polycistronic microRNA in wheat. Plant Biotechnol J. doi: 10. 1111/j. 1467-7652. 2011. 00647. x.
8. Fernandez AI, Viron N, Alhagdow M, Karimi M, Jones M, Amsellem Z, Sicard A, Czerdenik A, Angenent G, Grierson D, May S, Seymour G, Eshed Y, Lemaire-Chamley M, Rothan C, Hilson P (2009) Flexible tools for gene expression and silencing in tomato. Plant Physiol 151: 1729-1740.
9. Gambino G, Gribaudo I, Leopold S, Schartl A, Laimer M (2005) Molecular characterization of grapevine plants transformed with GFLV resistance genes: I. Plant Cell Rep 24: 655-662.
10. Gambino G, Perrone I, Carra A, Chitarra W, Boccacci P, Torello Marinoni D, Barberis M, Maghuly F, Laimer M, Gribaudo I (2010) Transgene silencing in grapevines transformed with GFLV resistance genes: analysis of variable expression of transgene, siRNAs production and cytosine methylation. Transgenic Res 19: 17-27.
11. Gölles R, da Câmara Machado A, Minafra A, Savino V, Saldarelli G, Martelli GP, Pühringer H, Katinger H, Laimer da Câmara Machado M (2000) Transgenic grapevines expressing coat protein gene sequences of Grapevine fanleaf virus, Arabis mosaic virus, Grapevine virus A and Grapevine virus B. Acta Hort 528: 305-311.
12. Jardak-Jamoussi R, Winterhagen P, Bouamama B, Dubois C, Mliki A, Wetzel T, Ghorbel A, Reustle GM (2009) Development and evaluation of GFLV inverted repeat construct for genetic transformation of grapevine. Plant Cell Tissue Organ Cult 97: 187-196.
13. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387-405.
14. Jiang F, Song Y, Han Q, Zhu C, Wen F (2011) The choice of target site is crucial in artificial miRNA-mediated virus resistance in transgenic Nicotiana tabacum. Physiol Mol Plant Pathol 76: 2-8.
15. Jovel J, Walker M, Sanfaçon M (2007) Recovery of Nicotiana benthamiana plants from a necrotic response induced by a Nepovirus is associated with RNA silencing but not with reduced virus titer. J Virol 81: 12285-12297.
16. Karimi M, Inze D, Depicker A (2002) GATEWAY vectors for Agrobacterium-mediated plant transformation. Trends Plant Sci 7: 193-195.
17. Kertesz M, Iovino N, Unnerstall U, Gaul U, Segal E (2007) The role of site accessibility in microRNA target recognition. Nat Genetics 39: 1278-1284.
18. Khraiwesh B, Ossowski S, Weigel D, Reski R, Frank W (2008) Specific gene silencing by artificial microRNAs in Physcomitrella patens: an alternative to targeted gene knockouts. Plant Physiol 148: 684-693.
19. Kim J, Somers DE (2010) Rapid assessment of gene function in the circadian clock using artificial microRNA in Arabidopsis mesophyll protoplasts. Plant Physiol 154: 611-621.
20. Krastanova S, Perrin M, Barbier P, Demangeat G, Cornuet P, Bardonnet N, Otten L, Pinck L, Walter B (1995) Transformation of grapevine rootstocks with the coat protein gene of Grapevine fanleaf Nepovirus. Plant Cell Rep 14: 550-554.
21. Li ZT, Dhekney SA, Dutt M, Gray DJ (2008) An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.). Plant Cell Tissue Organ Cult 93: 311-321.
22. Maghuly F, Leopold S, Da Câmara Machado A, Borroto Fernandez E, Ali Khan M, Gambino G, Gribaudo I, Schartl A, Laimer M (2006) Molecular characterization of grapevine plants transformed with GFLV resistance genes: II. Plant Cell Rep 25: 546-553.
23. Maillot P, Lebel S, Schellenbaum P, Jacques A, Walter B (2009) Differential regulation of SERK, LEC1-like and Pathogenesis-Related genes during indirect secondary somatic embryogenesis in grapevine. Plant Physiol Biochem 47: 743-752.
24. Martelli GP (1978) Nematode-borne viruses of grapevine, their epidemiology and control. Nematol Med 6: 1-27.
25. Mauro MC, Toutain A, Walter B, Pinck L, Otten L, Coutos-Thevenot P, Deloire A, Barbier P (1995) High efficiency regeneration of grapevine plants transformed with the GFLV coat protein gene. Plant Sci 112: 97-106.
26. Mica E, Piccolo V, Delledonne M, Ferrarini A, Pezzotti M, Casati C, Del Fabbro C, Valle G, Policrit A, Morgante M, Pesole G, Pè ME, Horner DS (2010) Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera. BMC Genomics 11: 109.
27. Molnar A, Bassett A, Thuenemann E, Schwach F, Karkare S, Ossowski S, Weigel D, Baulcombe D (2009) Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J 58: 165-174.
28. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol Plant 15: 493-497.
29. Niu QW, Lin SS, Reyes JL, Chen KC, Wu HW, Yeh SD, Chua NH (2006) Expresion of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Nat Biotechnol 24: 1420-1428.
30. Papp I, Mette MF, Aufsatz W, Daxinger L, Schauer SE, Ray A, van der Winden J, Matzke M, Matzke AJ (2003) Evidence for nuclear processing of plant microRNA and short interfering RNA precursors. Plant Physiol 132: 1382-1390.
31. Qu J, Ye J, Fang R (2007) Artificial microRNA-mediated virus resistance in plants. J Virol 81: 6690-6699.
32. RDC Team (2011) R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing. http://www. r-project. org. Accessed 09 Sept 2011.
33. Ritzenthaler C, Viry M, Pinck M, Margis R, Fuchs M, Pinck L (1991) Complete nucleotide sequence and genetic organization of Grapevine fanleaf Nepovirus RNA1. J Virol 72: 2357-2365.
34. Salomé PA, Weigel D, McClung CR (2010) The role of the Arabidopsis morning loop components CCA1, LHY, PRR7, and PRR9 in temperature compensation. Plant Cell 22: 3650-3661.
35. Sanfaçon H, Wellink J, Le Gall O, Karasev A, van der Vlugt R, Wetzel T (2009) Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus. Arch Virol 154: 899-907.
36. Santos-Rosa M, Poutaraud A, Merdinoglu D, Mestre P (2008) Development of a transient expression system in grapevine via agro-infiltration. Plant Cell Rep 27: 1053-1063.
37. Schellenbaum P, Jacques A, Maillot P, Bertsch C, Mazet F, Farine S, Walter B (2008) Characterization of VvSERK1, VvSERK2, VvSERK3 and VvL1L genes and their expression during somatic embryogenesis of grapevine (Vitis vinifera L.). Plant Cell Rep 27: 1799-1809.
38. Schwab R, Ossowski S, Riester M, Warthmann N, Weigel D (2006) Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18: 1121-1133.
39. Serghini MA, Fuchs M, Pinck M, Reinbolt J, Walter B, Pinck L (1990) RNA2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location. J Gen Virol 71: 1433-1441.
40. Shi R, Yang C, Lu S, Sederoff R (2010) Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa. Planta 232: 1281-1288.
41. Tang Y, Wang F, Zhao J, Xie K, Hong Y, Liu Y (2010) Virus-based microRNA expression for gene functional analysis in plants. Plant Physiol 153: 632-641.
42. Valat L, Fuchs M, Burrus M (2006) Transgenic grapevine rootstock clones expressing the coat protein or movement protein genes of Grapevine fanleaf virus: Characterization and reaction to virus infection upon protoplast electroporation. Plant Sci 170: 739-747.
43. Varkonyi-Gasic E, Wu R, Wood M, Walton EF, Hellens RP (2007) Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs. Plant Methods 3: 12.
44. Vervliet G, Holsters M, Teuchy H, van Montagu M, Schell J (1975) Characterization of different plaque-forming and defective temperate phages in Agrobacterium. J Gen Virol 26: 33-48.
45. Vigne E, Komar V, Fuchs M (2004) Field safety assessment of recombination in transgenic grapevines. Transgenic Res 13: 165-179.
46. Warthmann N, Chen H, Ossowski S, Weigel D, Hervé P (2008) Highly specific gene silencing by artificial miARNs in rice. PLoS ONE 3: e1829.
47. Wassenegger M, Krczal G (2006) Nomenclature and functions of RNA-directed RNA polymerases. Trends Plant Sci 11: 142-151.
48. Wroblewski T, Tomczak A, Michelmore R (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis. Plant Biotechnol J 3: 259-273.
49. Xue B, Ling KS, Reid CL, Krastanova S, Sekiya M, Momol EA, Süle S, Mozsar J, Gonsalves D, Burr TJ (1999) Transformation of five grape rootstocks with plant virus genes and a virE2 gene from Agrobacterium tumefaciens. In Vitro Cell Dev Biol Plant 35: 226-231.
50. Zhang X, Li H, Zhang J, Zhang C, Gong P, Ziaf K, Xiao F, Ye Z (2011) Expression of artificial microRNAs in tomato confers efficient and stable virus resistance in a cell-autonomous manner. Transgenic Res 20: 569-581.
51. Zottini M, Barizza E, Costa A, Formentin E, Ruberti C, Carimi F, Lo Schiavo F (2008) Agroinfiltration of grapevine leaves for fast transient assays of gene expression and for long-term production of stable transformed cells. Plant Cell Rep 27: 845-853.