Small interfering RNA targeted to secretory clusterin blocks tumor growth, motility, and invasion in breast cancer

Acta Biochimica et Biophysica Sinica

Volumn 44, Issue 12, 2012, Pages 991-998

  Niu, Zhaohe ^a   Li, Xinhui ^a   Hu, Bin ^a   Li, Rong ^a   Wang, Ligang ^b   Wu, Lilin ^b   Wang, Xingang ^a  

^a QINGDAO UNIVERSITY   (China)

^b Tianjin Union Medicine Centre   (China)

Author keywords

apoptosis; breast cancer; clusterin; gene treatment; metastasis; siRNAs

Indexed keywords

CASPASE 3; CLUSTERIN; KI 67 ANTIGEN; SMALL INTERFERING RNA;

ANIMAL; APOPTOSIS; ARTICLE; BREAST TUMOR; CANCER INVASION; CELL MOTION; CELL PROLIFERATION; CELL STRAIN HEK293; CELL SURVIVAL; EXPERIMENTAL NEOPLASM; FEMALE; GENETICS; HUMAN; LUNG TUMOR; METABOLISM; METASTASIS; MOUSE; NUDE MOUSE; PATHOLOGY; RNA INTERFERENCE; TUMOR CELL LINE; TUMOR VOLUME; XENOGRAFT;

ANIMALS; APOPTOSIS; BREAST NEOPLASMS; CASPASE 3; CELL LINE, TUMOR; CELL MOVEMENT; CELL PROLIFERATION; CELL SURVIVAL; CLUSTERIN; FEMALE; HEK293 CELLS; HUMANS; KI-67 ANTIGEN; LUNG NEOPLASMS; MAMMARY NEOPLASMS, EXPERIMENTAL; MICE; MICE, NUDE; NEOPLASM INVASIVENESS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSPLANTATION, HETEROLOGOUS; TUMOR BURDEN;

RODENTIA;

EID: 84870213761     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gms091     Document Type: Article

References (44)

1. Rosenberg ME and Silkensen J. Clusterin: physiologic and pathophysiologic considerations. Int J Biochem Cell Biol 1995, 27: 633-645.
2. Lakins J, Bennett SA and Chen JH. Clusterin biogenesis is altered during apoptosis in the regressing rat ventral prostate. J Biol Chem 1998, 273: 27887-27895.
3. Reddy KB, Jin G and Karode MC. Transforming growth factor beta (TGF beta)-induced nuclear localization of apolipoprotein J/clusterin in epithelial cells. Biochemistry 1996, 35: 6157-6163.
4. Yang CR, Leskov K and Hosley-Eberlein K. Nuclear clusterin/XIP8, an X-ray-induced Ku70-binding protein that signals cell death. Proc Natl Acad Sci USA 2000, 97: 5907-5912.
5. Caccamo AE, Scaltriti M and Caporali A. Cell detachment and apoptosis induction of immortalized human prostate epithelial cells are associated with early accumulation of a 45 kDa nuclear isoform of clusterin. Biochem J 2004, 382: 157-168.
6. Jones SE and Jomary C. Clusterin. Int J Biochem Cell Biol 2002, 34: 427-431.
7. O'Sullivan J, Whyte L and Drake J. Alterations in the post-translational modification and intracellular trafficking of clusterin in MCF-7 cells during apoptosis. Cell Death Differ 2003, 10: 914-927.
8. Criswell T, Klokov D and Beman M. Repression of IR inducible clusterin expression by the p53 tumor suppressor protein. Cancer Biol Ther 2003, 2: 372-380.
9. Debure L, Vayssiere JL and Rincheval V. Intracellular clusterin causes juxtanuclear aggregate formation and mitochondrial alteration. J Cell Sci 2003, 116: 3109-3121.
10. Scaltriti M, Santamaria A, Paciucci R and Bettuzzi S. Intracellular clusterin induces G2-M phase arrest and cell death in PC-3 prostate cancer cells. Cancer Res 2004, 64: 6174-6182.
11. Miyake H, Hara I and Kamidono S. Synergistic chemsensitization and inhibition of tumor growth and metastasis by the antisense oligodeoxynucleotide targeting clusterin gene in a human bladder cancer model. Urology 2001, 7: 4245-4252.
12. Gleave ME, Miyake H and Zellweger T. Use of antisense oligonucleotides targeting the antiapoptotic gene, clusterin/testosterone-repressed prostate message 2, to enhance androgen sensitivity and chemosensitivity in prostate cancer. Urology 2001, 58: 39-49.
13. Kang YK, Hong SW, Lee H and Kim WH. Overexpression of clusterin in human hepatocellular carcinoma. Hum Pathol 2004, 35: 1340-1346.
14. Lau SH, Sham JS, Xie D, Tzang CH, Tang D, Ma N and Hu L, et al. Clusterin plays an important role in hepatocellular carcinoma metastasis. Oncogene 2006, 25: 1242-1250.
15. Lokamani I, Looi ML, Ali SA, Dali AZ and Jamal R. Clusterin as a potential marker in distinguishing cervical neoplasia. Anal Quant Cytol Histol 2011, 33: 223-228.
16. Bi J, Guo AL, Lai YR, Li B, Zhong JM, Wu HQ and Xie Z, et al. Overexpression of clusterin correlates with tumor progression, metastasis in gastric cancer: a study on tissue microarrays. Neoplasma 2010, 57: 191-197.
17. Yang GF, Li XM and Xie D. Overexpression of clusterin in ovarian cancer is correlated with impaired survival. Int J Gynecol Cancer 2009, 19: 1342-1346.
18. Hazzaa SM, Elashry OM and Afifi IK. Clusterin as a diagnostic and prognostic marker for transitional cell carcinoma of the bladder. Pathol Oncol Res 2010, 16: 101-109.
19. Mourra N, Couvelard A, Tiret E, Olschwang S and Flejou JF. Clusterin is highly expressed in pancreatic endocrine tumours but not in solid pseudopapillary tumours. Histopathology 2007, 50: 331-337.
20. Xie MJ, Motoo Y, Su SB, Mouri H, Ohtsubo K, Matsubara F and Sawabu N. Expression of clusterin in human pancreatic cancer. Pancreas 2002, 25: 234-238.
21. Rizzi F and Bettuzzi S. Clusterin (CLU) and prostate cancer. Adv Cancer Res 2009, 105: 1-19.
22. Redondo M, Villar E, Torres-Muñoz J, Tellez T, Morell M and Petito CK. Overexpression of clusterin in human breast carcinoma. Am J Pathol 2000, 157: 393-399.
23. Sensibar JA, Sutkowski DM, Raffo A, Buttyan R, Griswold MD, Sylvester SR and Kozlowski JM, et al. Prevention of cell death induced by tumor necrosis factor alpha in LNCaP cells by overexpression of sulfated glycoprotein- 2(clusterin). Cancer Res 1995, 55: 2431-2437.
24. Humphreys D, Hochgrebe TT, Easterbrook-Smith SB, Tenniswood MP and Wilson MR. Effects of clusterin overexpression on TNFalpha-and TGFbeta-mediated death of L929 cells. Biochemistry 1997, 36: 15233-15243.
25. Sintich SM, Steinberg J, Kozlowski JM, Lee C, Pruden S, Sayeed S and Sensibar JA. Cytotoxic sensitivity to tumor necrosis factor-alpha in PC3 and LNCaP prostatic cancer cells is regulated by extracellular levels of SGP-2 (clusterin). Prostate 1999, 39: 87-93.
26. Zhan P, Lee EC, Packman K and Tenniswood M. Induction of invasive phenotype by Casodex in hormone-sensitive prostate cancer cells. J Steroid Biochem Mol Biol 2002, 83: 101-111.
27. Lee EC, Zhan P, Schallhorn R, Packman K and Tenniswood M. Antiandrogeninduced cell death in LNCaP human prostate cancer cells. Cell Death Diff 2003, 10: 761-771.
28. Flanagan L, Whyte L, Chatterjee N and Tenniswood M. Effects of clusterin over-expression on metastatic progression and therapy in breast cancer. BMC Cancer 2010, 10: 107.
29. Doustjalali SR, Yusof R, Yip CH, Looi LM, Pillay B and Hashim OH. Aberrant expression of acute phase reactant proteins in sera and breast lesions of patients with malignant and benign tumors. Electrophoresis 2004, 25: 2392-2401.
30. Sutton D, Kim S, Shuai X, Leskov K, Marques JT, Williams BR and Boothman DA, et al. Efficient suppression of secretory clusterin levels by polymer-siRNA nanocomplexes enhances ionizing radiation lethality in human MCF-7 breast cancer cells in vitro. Int J Nanomed 2006, 1: 155-162.
31. So A, Sinnemann S, Huntsman D, Fazli L and Gleave M. Knockdown of the cytoprotective chaperone, clusterin, chemosensitizes human breast cancer cells both in vitro and in vivo. Mol Cancer Ther 2005, 4: 1837-1849.
32. Shannan B, Seifert M, Leskov K, Willis J, Boothman D, Tilgen W and Reichrath J. Challenge and promise: roles for clusterin in pathogenesis, progression and therapy of cancer. Cell Death Differ 2006, 13: 12-19.
33. Miyake H, Gleave ME, Arakawa S, Kamidono S and Hara I. Introducing the clusterin gene into human renal cell carcinoma cells enhances their metastatic potential. J Urol 2002, 167: 2203-2208.
34. Yang D, Song X, Zhang J, Ye L, Wang S, Che X and Wang J, et al. Therapeutic potential of siRNA-mediated combined knockdown of the IAP genes (Livin, XIAP, and Survivin) on human bladder cancer T24 cells. Acta Biochim Biophys Sin 2010, 42: 137-144.
35. Guo F, Li Y, Liu Y, Wang J, Li Y and Li G. Inhibition of metastasis-associated lung adenocarcinoma transcript 1 in CaSki human cervical cancer cells suppresses cell proliferation and invasion. Acta Biochim Biophys Sin 2010, 42: 224-229.
36. Meng J, Xie W, Cao L, Hu C and Zhe Z. shRNA targeting HDGF suppressed cell growth and invasion of squamous cell lung cancer. Acta Biochim Biophys Sin 2010, 42: 52-57.
37. Wei L, Xue T, Wang J, Chen B, Lei Y, Huang Y and Wang H, et al. Roles of clusterin in progression, chemoresistance and metastasis of human ovarian cancer. Int J Cancer 2009, 125: 791-806.
38. Santilli G, Aronow BJ and Sala A. Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity. J Biol Chem 2003, 278: 38214-38219.
39. Moretti RM, Marelli MM, Mai S, Cariboni A, Scaltriti M, Bettuzzi S and Limonta P. Clusterin isoforms differentially affect growth and motility of prostate cells: possible implications in prostate tumorigenesis. Cancer Res 2007, 67: 10325-10333.
40. Redondo M, Tellez T and Roldan MJ. The role of clusterin (CLU) in malignant transformation and drug resistance in breast carcinomas. Adv Cancer Res 2009, 105: 21-43.
41. Lee C, Janulis L, Ilio K, Shah A, Park I, Kim S and Cryns V, et al. In vitro models of prostate apoptosis: clusterin as an antiapoptotic mediator. Prostate Suppl 2000, 9: 21-24.
42. Hara I, Miyake H, Gleave ME and Kamidono S. Introduction of clusterin gene into human renal cell carcinoma cells enhances their resistance to cytotoxic chemotherapy through inhibition of apoptosis both in vitro and in vivo. Jpn J Cancer Res 2001, 92: 1220-1224.
43. Trougakos IP, So A, Jansen B, Gleave ME and Gonos ES. Sliencing expression of the clusterin/apolipoprotein j gene in human cancer cells using small interfering RNA induces spontaneous apoptosis, reduced growth ability, and cell sensitization to genotoxic and oxidative stress. Cancer Res 2004, 64: 1834-1842.
44. Garden GA, Bothwell M and Rubel EW. Lack of correspondence between mRNA expression for a putative cell death molecule (SGP-2) and neuronal cell death in the central nervous system. J Neurobiol 1991, 22: 590-604.