Evaluation of two commercial and three home-made fixatives for the substitution of formalin: A formaldehyde-free laboratory is possible

Environmental Health: A Global Access Science Source

Volumn 11, Issue 1, 2012, Pages

  Zanini, Cristina ^a   Gerbaudo, Elisa ^b   Ercole, Elisabetta ^a   Vendramin, Anna ^c   Forni, Marco ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b REGINA MARGHERITA CHILDREN S HOSPITAL   (Italy)

^c EuroClone SpA   (Italy)

Author keywords

Alternative fixatives; Formalin toxicity; RNA extraction; Tissue micro array

Indexed keywords

ACETIC ACID; ALCOHOL; FIXATIVE; FORMALDEHYDE; GLYCEROL; MACROGOL; NUCLEIC ACID;

BIOCHEMISTRY; CARCINOGEN; DILUTION; FORMALDEHYDE; LABORATORY METHOD; NINETEENTH CENTURY; NUCLEIC ACID; PATHOLOGY; PROTEIN; RNA;

ARTICLE; IMMUNOHISTOCHEMISTRY; LABORATORY; MORPHOLOGY; PRIORITY JOURNAL; RNA EXTRACTION; TISSUE MICROARRAY;

AIR POLLUTANTS; AIR POLLUTION; CARCINOGENS; FIXATIVES; FORMALDEHYDE; LABORATORIES; TISSUE FIXATION;

EID: 84870168681     PISSN: None     EISSN: 1476069X     Source Type: Journal    
DOI: 10.1186/1476-069X-11-59     Document Type: Article

References (45)

1. Histological and Histochemical Methods. Kiernan JA, Theory and Practice Scion Publishing, Bloxham, UK, Kiernan JA, 4 2008 12-44 431-437
2. The use of Formalin in Neurology. Fish PA, Trans Am Microsc Soc 1896 17 319 330
3. Fixation conditions for DNA and RNA in situ hybridization: a reassessment of molecular morphology dogma. Tbakhi A, Totos G, Hauser-Kronberger C, Pettay J, Baunoch D, Hacker GW, Tubbs RR, Am J Pathol 1998 152 35 41 9422521 (Pubitemid 28028044)
4. Establishment of the Formalin-free Surgical Pathology Laboratory. Bostwick DC, Al Anouf N, Choi C, Arch Pathol Lab Med 1994 118 298 302 8135636
5. U.S. Environmental Protection Agency, Office of Air and Radiation, Report to Congress on Indoor Air Quality, Volume II Chapter 4: Assessment and Control of Indoor Air Pollution 1989 12 27
6. Response to Court remand; final rule. Occupational exposure to Formaldehyde - OSHA, 29 CFR 1910, Federal Register 1992 50 22290 22328
7. Meeting report: summary of IARC monographs on formaldehyde, 2-butoxyethanol, and 1-tert-butoxy-2-propanol. Cogliano VJ, Grosse Y, Baan RA, Straif K, Secretan MB, El Ghissassi F, Environ Health Perspect 2005 113 1205 1208 10.1289/ehp.7542 16140628 (Pubitemid 41387660)
8. WHO International Agency for Research on Cancer Monograph Working Group: A review of human carcinogens - Part F: chemical agents and related occupations. Baan R, Grosse Y, Straif K, Secretan B, El Ghissassi F, Bouvard V, Benbrahim-Tallaa L, Guha N, Freeman C, Galichet L, Cogliano V, Lancet Oncol 2009 10 1143 1144 10.1016/S1470-2045(09)70358-4 19998521
9. A Review of Human Carcinogens: Chemical Agents and Related Occupations. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Volume 100F, Formaldehyde 2012 http://monographs.iarc.fr/ENG/Monographs/vol100F/mono100F-29. pdf
10. Toxic and hazardous sub-stances. Occupational Safety and Health Administration, Formaldehyde Occupational Safety and Health Administration, Code of Federal Regulations 2007 1910 1048 29 CFR
11. Exposure to Formaldehyde and Its Potential Human Health Hazards. Kim KH, Jahan SA, Lee JT, J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2011 29 277 299 22107164
12. DNA-protein crosslinks and p53 protein expression in relation to occupational exposure to formaldehyde. Shaham J, Bomstein Y, Gurvich R, Rashkovsky M, Kaufman Z, Occup Environ Med 2003 60 403 409 10.1136/oem.60.6.403 12771391 (Pubitemid 36724193)
13. Combined analysis of chromosomal aberrations and glutathione S-transferase M1 and T1 polymorphisms in pathologists occupationally exposed to formaldehyde. Santovito A, Schilir T, Castellano S, Cervella P, Bigatti MP, Gilli G, Bono R, DelPero M, Arch Toxicol 2011 85 1295 1302 10.1007/s00204-011- 0668-3 21360194
14. Histology without formalin? Buesa RJ, Ann Diagn Pathol 2008 12 387 396 10.1016/j.anndiagpath.2008.07.004 18995201
15. Malondialdehyde-deoxyguanosine adduct formation in workers of pathology wards: the role of air formaldehyde exposure. Bono R, Romanazzi V, Munnia A, Piro S, Allione A, Ricceri F, Guarrera S, Pignata C, Matullo G, Wang P, Giese RW, Peluso M, Chem Res Toxicol 2010 23 1342 1348 10.1021/tx100083x 20707408
16. Formaldehyde and tobacco smoke as alkylating agents: the formation of N-methylenvaline in pathologists and in plastic laminate workers. Bono R, Romanazzi V, Pirro V, Degan R, Pignata C, Suppo E, Pazzi M, Vincenti M, Sci Total Environ 2012 414 701 707 22104379
17. Histomorphologic Assessment of Formalin substitute Fixatives for Diagnostic Surgical Pathology. Titford ME, Horenstein MG, Arch Pathol Lab Med 2005 129 502 506 15794674 (Pubitemid 40471197)
18. Histomorphometric comparison after fixation with formaldehyde or glyoxal. Wang YN, Lee K, Pai S, Ledoux WR, Biotech Histochem 2011 86 359 365 10.3109/10520295.2010.520275 20854226
19. NATIONAL TOXICOLOGY PROGRAM, USA, Specifications for conduct of studies to evaluate the toxic and Carcinogenic Potential of Chemical, Biological and Physical Agents in Laboratory Animals for the NATIONAL TOXICOLOGY PROGRAM 2011 accessible as PDF at: http://ntp.niehs.nih.gov/ntp/Test-Info/FinalNTP- ToxCarSpecsJan2011.pdf
20. First Experimental Demonstration of the Multipotential Carcinogenic Effects of Aspartame administered in feed to Sprague-Dawley rats. Soffritti M, Belpoggi F, Degli Espositi D, Lambertiti L, Tibaldi E, Rigano A, Environ Health Perspect 2006 114 379 385 16507461 (Pubitemid 43338263)
21. Evaluation of non-formalin tissue fixation for molecular profiling studies. Gillespie JW, Best CJ, Bichsel VE, Cole KA, Greenhut SF, Hewitt SM, Ahram M, Gathright YB, Merino MJ, Strausberg RL, Epstein JI, Hamilton SR, Gannot G, Baibakova GV, Calvert VS, Flaig MJ, Chuaqui RF, Herring JC, Pfeifer J, Petricoin EF, Linehan WM, Duray PH, Bova GS, Emmert-Buck MR, Am J Pathol 2002 160 449 457 10.1016/S0002-9440(10)64864-X 11839565 (Pubitemid 34160322)
22. An alternative fixative to formalin fixation for molecular applications: the RCL2()-CS100 approach. Denoul A, Boissière-Michot F, Rochaix P, Bibeau F, Boulle N, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols, Methods in Molecular Biology. vol 724 GFR Berlin: Springer, Al-Mulla F, 2011 297 307
23. Morphological quality and nucleic acid preservation in cytopathology. Gazziero A, Guzzardo V, Aldighieri E, Fassina A, J Clin Pathol 2009 62 429 434 10.1136/jcp.2008.059808 18818264
24. Fixation of brain tumor biopsy specimens with RCL2 results in well-preserved histomorphology, immunohistochemistry and nucleic acids. Preusser M, Plumer S, Dirnberger E, Hainfellner JA, Mannhalter C, Brain Pathol 2010 20 1010 1020 10.1111/j.1750-3639.2010.00400.x 20477829
25. Formaldehyde substitute fixatives: effects on nucleic acid preservation. Moelans CB, Oostenrijk D, Moons MJ, van Diest PJ, J Clin Pathol 2011 64 960 967 10.1136/jclinpath-2011-200152 21715573
26. On the chemistry of formaldehyde fixation and its effects on immunohistochemical reactions. Puchtler H, Meloan SN, Histochemistry 1985 82 201 204 10.1007/BF00501395 3997553 (Pubitemid 15114056)
27. Antibodies that label paraffin-embedded mouse tissues: a collaborative endeavor. Mikaelian I, Nanney LB, Parman KS, Kusewitt DF, Ward JM, Näf D, Krupke DM, Eppig JT, Bult CJ, Seymour R, Ichiki T, Sundberg JP, Toxicol Pathol 2004 32 181 191 10.1080/01926230490274335 15200156 (Pubitemid 38437547)
28. Ki-67 Antigen Retrieval in Tissue and Cells. Suurmeijer AJH, Boon ME, Antigen Retrieval Tecniques: Immunohistochemistry and Molecular Morphology Eaton Publishing, Natick, MA, Shi S-R, Gu J, Taylor CR, 2000 193 205
29. Effects of preanalytical variables on the detection of proteins by immunohistochemistry in formalin-fixed, paraffin-embedded tissue. Engel KB, Moore HM, Arch Pathol Lab Med 2011 135 537 543 21526952
30. Zinc-Based Fixative Improves Preservation of Genomic DNA and Proteins in Histoprocessing of Human Tissues. Wester K, Asplund A, Bäckvall H, Micke P, Derveniece A, Hartmane I, Malmström PU, Pontén F, Lab Invest 2003 83 889 899 12808124 (Pubitemid 36723698)
31. Novel zinc-based fixative for high quality DNA, RNA and protein analysis. Lykidis D, Van Noorden S, Armstrong A, Spencer-Dene B, Li J, Zhuang Z, Stamp GW, Nucleic Acids Res 2007 35 85 10.1093/nar/gkm433 17576663 (Pubitemid 47248461)
32. Building Sustainable Capacity for Disease Diagnosis in Sub-Saharan Africa: Case Studies of Cooperation in Diagnostic Pathology. Mariani-Costantini R, Awadelkarim KD, Barberis M, et al. New Knowledge in a New Era of Globalization Rijeka, Croatia: ImTech, Pachura P, 2011 243 266
33. Tissue transfer to pathology labs: under vacuum is the safe alternative to formalin. Bussolati G, Chiusa L, Cimino A, DArmento G, Virchows Arch 2008 452 229 231 10.1007/s00428-007-0529-x 18097686
34. A useful individual protection system for the Gross Room and autopsy activity of the Pathologist. Pagani A, Pratesi M, Vechiè V, Iandolo M, Vigliani R, Pathologica 2009 101 263 264 20387717
35. European Community REGULATION No 1907 2006 available as PDF at: http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=oj:l:2006:396:0001:0849: en:pdf
36. Appendix: Antigen Retrieval Technique. Shi S-R, Cote RJ, Shi Y, Taylor CR, Antigen Retrieval Tecniques: Immunohistochemistry and Molecular Morphology Natick, MA: Eaton Publishing, Shi S-R, Gu J, Taylor CR, 2000 311 333
37. Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein. Giaccone G, Canciani B, Puoti G, Rossi G, Goffredo D, Iussich S, Fociani P, Tagliavini F, Bugiani O, Brain Pathol 2000 10 31 37 10668893 (Pubitemid 30056391)
38. RNA extraction from formalin-fixed and paraffin-embedded tissues. Stanta G, Bonin S, Perin R, Methods Mol Biol 1998 86 23 26 9664448
39. Formaldehyde substitute fixatives. Analysis of macroscopy, morphologic analysis, and immunohistochemical analysis. Moelans CB, ter Hoeve N, van Ginkel JW, ten Kate FJ, van Diest PJ, Am J Clin Pathol 2011 136 548 556 10.1309/AJCPHH1B0COCBGOM 21917676
40. Comparison of formalin-free tissue fixatives: a proteomic study testing their application for routine pathology and research. Kothmaier H, Rohrer D, Stacher E, Quehenberger F, Becker KF, Popper HH, Arch Pathol Lab Med 2011 135 744 752 21631267
41. La Formalina come mezzo di fissazione in sostituzione dell'acido osmico nel metodo di Ramn y Cajal. Lachi P, Anat Anz 1895 X 790 791
42. American Society of Clinical Oncology/College of American Pathologists: American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. Wolff AC, Hammond ME, Schwartz JN, Hagerty KL, Allred DC, Cote RJ, Dowsett M, Fitzgibbons PL, Hanna WM, Langer A, McShane LM, Paik S, Pegram MD, Perez EA, Press MF, Rhodes A, Sturgeon C, Taube SE, Tubbs R, Vance GH, van de Vijver M, Wheeler TM, Hayes DF, Arch Pathol Lab Med 2007 131 18 43 19548375 (Pubitemid 46174133)
43. Current issues in ER and HER2 testing by IHC in breast cancer. Gown AM, Mod Pathol 2008 21 Suppl 2 8 S15 18437174 (Pubitemid 351592775)
44. Guidelines for human epidermal growth factor receptor 2 testing: biologic and methodologic considerations. Sauter G, Lee J, Bartlett JM, Slamon DJ, Press MF, J Clin Oncol 2009 27 1323 1333 10.1200/JCO.2007.14.8197 19204209
45. A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Vincek V, Nassiri M, Nadji M, Morales AR, Lab Invest 2003 83 1427 1435 10.1097/01.LAB.0000090154.55436.D1 14563944 (Pubitemid 37267826)