Multi-enzymatic one-pot reduction of dehydrocholic acid to 12-keto-ursodeoxycholic acid with whole-cell biocatalysts

Biotechnology and Bioengineering

Volumn 110, Issue 1, 2013, Pages 68-77

  Sun, Boqiao ^a   Kantzow, Christina ^a   Bresch, Sven ^a   Castiglione, Kathrin ^a   Weuster Botz, Dirk ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Biocatalysis; Cofactor regeneration; Hydroxysteroid dehydrogenase; Ursodeoxycholic acid; Whole cell biotransformation

Indexed keywords

BIOCATALYSIS; COFACTOR REGENERATION; HYDROXYSTEROID DEHYDROGENASE; URSODEOXYCHOLIC ACID; WHOLE-CELL BIOTRANSFORMATIONS;

BATCH DATA PROCESSING; ENZYMES; ESCHERICHIA COLI; GLUCOSE;

BIOCATALYSTS;

2 KETO URSODEOXYCHOLIC ACID; 3ALPHA HYDROXYSTEROID DEHYDROGENASE; DEHYDROCHOLIC ACID; FORMATE DEHYDROGENASE; GLUCOSE DEHYDROGENASE; TESTOSTERONE 17BETA DEHYDROGENASE; UNCLASSIFIED DRUG; URSODEOXYCHOLIC ACID;

ARTICLE; BACILLUS SUBTILIS; BACTERIUM; BATCH PROCESS; BIOCATALYST; COLLINSELLA AEROFACIEN; COMAMONAS TESTOSTERONI; ESCHERICHIA COLI; HOST; MUTANT; MYCOBACTERIUM VACCAE; NONHUMAN; ONE POT SYNTHESIS; REDUCTION KINETICS; WHOLE CELL BIOCATALYST;

ACTINOBACTERIA; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BIOCATALYSIS; BIOREACTORS; BIOTECHNOLOGY; CLONING, MOLECULAR; COMAMONAS; DEHYDROCHOLIC ACID; ESCHERICHIA COLI; HYDROXYSTEROID DEHYDROGENASES; MYCOBACTERIUM; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; URSODEOXYCHOLIC ACID;

BACILLUS SUBTILIS; COLLINSELLA AEROFACIENS; COMAMONAS TESTOSTERONI; ESCHERICHIA COLI; MYCOBACTERIUM VACCAE;

EID: 84869829028     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24606     Document Type: Article

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