Isolation of cardiomyocyte nuclei from post-mortem tissue

Journal of Visualized Experiments

Volumn , Issue 65, 2012, Pages

  Bergmann, Olaf ^a   Jovinge, Stefan ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Cardiology; Cardiomyocyte; Flow cytometry; Medicine; Nuclei isolation; PCM 1; Pericentriolar material 1; Physiology; Post mortem; Stem Cell Biology; Tissue Engineering

Indexed keywords

EID: 84869819670     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4205     Document Type: Article

References (19)

1. Ang, K.L., et al. Limitations of conventional approaches to identify myocyte nuclei in histologic sections of the heart. American journal of physiology. Cell physiology. 298, C1603-1609, doi:10.1152/ajpcell.00435.2009 (2010).
2. Bergmann, O., et al. Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover. Experimental cell research. 327, 188-194 (2011).
3. Bergmann, O., et al. Evidence for Cardiomyocyte Renewal in Humans. Science. 324, 98-102, doi:10.1126/science.1164680 (2009).
4. Walsh, S., et al. Cardiomyocyte cell cycle control and growth estimation. Cardiovascular Research., 1-31 (2010).
5. Spalding, K., Bhardwaj, R.D., Buchholz, B., Druid, H., & Frisén, J. Retrospective birth dating of cells in humans. Cell. 122, 133-143, doi:10.1016/j.cell.2005.04.028 (2005).
6. Adler, C.P., Friedburg, H., Herget, G.W., Neuburger, M., & Schwalb, H. Variability of cardiomyocyte DNA content, ploidy level and nuclear number in mammalian hearts. Virchows Arch. 429, 159-164 (1996).
7. Herget, G.W., Neuburger, M., Plagwitz, R., & Adler, C.P. DNA content, ploidy level and number of nuclei in the human heart after myocardial infarction. Cardiovascular Research. 36, 45-51 (1997).
8. Adler, C.P. & Friedburg, H. Myocardial DNA content. ploidy level and cell number in geriatric hearts: postmortem examinations of human myocardium in old age. Mol. Cell Cardiol. 18, 3953 (1986).
9. Dubois, N.C., et al. SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells. Nature biotechnology. 29, 1011-1018, doi:10.1038/nbt.2005 (2011).
10. Hattori, F., et al. Nongenetic method for purifying stem cell-derived cardiomyocytes. Nature Methods. 7, 61-66, doi:10.1038/nmeth.1403 (2010).
11. Fransioli, J., et al. Evolution of the c-kit-Positive Cell Response to Pathological Challenge in the Myocardium. Stem Cells. 26, 1315-1324, doi:10.1634/stemcells.2007-0751 (2008).
12. Elliott, D.A., et al. NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nature Methods. 8, 1037-1040, doi:10.1038/nmeth.1740 (2011).
13. Laflamme, M.A. Evidence for Cardiomyocyte Repopulation by Extracardiac Progenitors in Transplanted Human Hearts. Circulation Research. 90, 634-640 (2002).
14. Srsen, V., Fant, X., Heald, R., Rabouille, C., & Merdes, A. Centrosome proteins form an insoluble perinuclear matrix during muscle cell differentiation. BMC cell biology. 10, 28 (2009).
15. Spoelgen, R., et al. A novel flow cytometry-based technique to measure adult neurogenesis in the brain. Journal of neurochemistry. 119, 165-175, doi:10.1111/j.1471-4159.2011.07413.x (2011).
16. Soonpaa, M.H., Kim, K.K., Pajak, L., Franklin, M., & Field, L.J. Cardiomyocyte DNA synthesis and binucleation during murine development. The American journal of physiology. 271, H2183-2189 (1996).
17. Olivetti, G., et al. Aging, cardiac hypertrophy and ischemic cardiomyopathy do not affect the proportion of mononucleated and multinucleated myocytes in the human heart. J Mol Cell Cardiol. 28, 1463-1477, doi:10.1006/jmcc.1996.0137 (1996).
18. Okada, S., et al. Flow cytometric sorting of neuronal and glial nuclei from central nervous system tissue. Journal of cellular physiology. 226, 552-558, doi:10.1002/jcp.22365 (2011).
19. Matevossian, A. & Akbarian, S. Neuronal Nuclei Isolation from Human Postmortem Brain Tissue. J. Vis. Exp. (20), e914, DOI: 10.3791/914 (2008).