Flow cytometric sorting of neuronal and glial nuclei from central nervous system tissue

Journal of Cellular Physiology

Volumn 226, Issue 2, 2011, Pages 552-558

  Okada, Seiji ^a   Saiwai, Hirokazu ^a   Kumamaru, Hiromi ^a   Kubota, Kensuke ^a   Harada, Akihito ^a   Yamaguchi, Masahiro ^b   Iwamoto, Yukihide ^a   Ohkawa, Yasuyuki ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS DNA; IMMUNOGLOBULIN F(AB) FRAGMENT; NUCLEOPROTEIN; RNA; TRANSCRIPTOME;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY LABELING; ARTICLE; BRAIN NUCLEUS; CENTRAL NERVOUS SYSTEM; CHROMATIN; CONTROLLED STUDY; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC REGULATION; KIDNEY; MOUSE; NERVE CELL; NONHUMAN; OLIGODENDROGLIA; PRIORITY JOURNAL; PROTEIN INTERACTION; SKELETAL MUSCLE; SPINAL CORD;

ANIMALS; CELL NUCLEUS; CELL SEPARATION; CENTRAL NERVOUS SYSTEM; FEMALE; FLOW CYTOMETRY; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; NEUROGLIA; NEURONS;

MUS MUSCULUS;

EID: 78649642465     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.22365     Document Type: Article

References (22)

1. Doyle JP, Dougherty JD, Heiman M, Schmidt EF, Stevens TR, Ma G, Bupp S, Shrestha P, Shah RD, Doughty ML, Gong S, Greengard P, Heintz N. 2008. Application of a translational profiling approach for the comparative analysis of CNS cell types. Cell 135: 749-762.
2. Heiman M, Schaefer A, Gong S, Peterson JD, Day M, Ramsey KE, Suárez-Fariñas M, Schwarz C, Stephan DA, Surmeier DJ, Greengard P, Heintz N. 2008. A translational profiling approach for the molecular characterization of CNS cell types. Cell 135: 738-748.
3. Jiang Y, Matevossian A, Huang HS, Straubhaar J, Akbarian S. 2008. Isolation of neuronal chromatin from brain tissue. BMC Neurosci 9: 42.
4. Kamme F, Salunga R, Yu J, Tran DT, Zhu J, Luo L, Bittner A, Guo HQ, Miller N, Wan J, Erlander M. 2003. Single-cell microarray analysis in hippocampus CA1: Demonstration and validation of cellular heterogeneity. J Neurosci 23: 3607-3615.
5. Kawaguchi A, Miyata T, Sawamoto K, Takashita N, Murayama A, Akamatsu W, Ogawa M, Okabe M, Tano Y, Goldman SA, Okano H. 2001. Nestin-EGFP transgenic mice: Visualization of the self-renewal and multipotency of CNS stem cells. Mol Cell Neurosci 17: 259-273.
6. Licatalosi DD, Darnell RB. 2010. RNA processing and its regulation: global insights into biological networks. Nat Rev Genet 11: 75-87.
7. Lobo MK, Karsten SL, Gray M, Geschwind DH, Yang XW. 2006. FACS-array profiling of striatal projection neuron subtypes in juvenile and adult mouse brains. Nat Neurosci 9: 443-452.
8. Lovtrup-Rein H, McEwen BS. 1966. Isolation and fractionation of rat brain nuclei. J Cell Biol 30: 405-415.
9. Matevossian A, Akbarian S. 2008. Neuronal nuclei isolation from human postmortem brain tissue. J Vis Exp 20.pii: 914. 10.3791/914.
10. Nigg EA. 1997. Nucleocytoplasmic transport: Signals, mechanisms and regulation. Nature 386: 779-787.
11. Ohkawa Y, Yoshimura S, Higashi C, Marfella CG, Dacwag CS, Tachibana T, Imbalzano AN. 2007. Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis. J Biol Chem 282: 6564-6570.
12. Pearson EC, Bates DL, Prospero TD, Thomas JO. 1984. Neuronal nuclei and glial nuclei from mammalian cerebral cortex. Nucleosome repeat lengths, DNA contents and H1 contents. Eur J Biochem 144: 353-360.
13. Sanz E, Yang L, Su T, Morris DR, McKnight GS, Amieux PS. 2009. Cell-type-specific isolation of ribosome-associated mRNA from complex tissues. Proc Natl Acad Sci USA 106: 13939-13944.
14. Siegmund KD, Connor CM, Campan M, Long TI, Weisenberger DJ, Biniszkiewicz D, Jaenisch R, Laird PW, Akbarian S. 2007. DNA methylation in the human cerebral cortex is dynamically regulated throughout the life span and involves differentiated neurons. PLoS ONE 2: e895.
15. Silver PA. 1991. How proteins enter the nucleus. Cell 64: 489-497.
16. Spalding KL, Bhardwaj RD, Buchholz BA, Druid H, Frisén J. 2005. Retrospective birth dating of cells in humans. Cell 122: 133-143.
17. Thompson RJ. 1973. Studies on RNA synthesis in two populations of nuclei from the mammalian cerebral cortex. J Neurochem 21: 19-40.
18. Tietjen I, Rihel JM, Cao Y, Koentges G, Zakhary L, Dulac C. 2003. Single-cell transcriptional analysis of neuronal progenitors. Neuron 38: 161-175.
19. Wang R, Brattain MG. 2007. The maximal size of protein to diffuse through the nuclear pore is larger than 60 kDa. FEBS Lett 581: 3164-3170.
20. Weis K. 2003. Regulating access to the genome: Nucleocytoplasmic transport throughout the cell cycle. Cell 112: 441-451.
21. Yamaguchi Y, Kim H, Kato K, Masuda K, Shimada I, Arata Y. 1995. Proteolytic fragmentation with high specificity of mouse immunoglobulin G. J Immunol Methods 181: 259-267.
22. Yamaguchi M, Saito H, Suzuki M, Mori K. 2000. Visualization of neurogenesis in the central nervous system using Nestin promoter-GFP transgenic mice. Neuroreport 11: 1991-1996.