Hypoxia Activated EGFR Signaling Induces Epithelial to Mesenchymal Transition (EMT)

PLoS ONE

Volumn 7, Issue 11, 2012, Pages

  Misra, Ashish ^a,b   Pandey, Chhiti ^a   Sze, Siu Kwan ^a   Thanabalu, Thirumaran ^a  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CETUXIMAB; EPIDERMAL GROWTH FACTOR RECEPTOR; RAC1 PROTEIN; UVOMORULIN; VINCULIN;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CELL ADHESION; CELL HYPOXIA; CELL MEMBRANE; CELL MIGRATION; CELL MOTILITY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME REGULATION; EPITHELIAL MESENCHYMAL TRANSITION; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; PHENOTYPE; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION;

ANTIBODIES, MONOCLONAL, HUMANIZED; CADHERINS; CELL ADHESION; CELL HYPOXIA; CELL LINE, TUMOR; CELL MOVEMENT; EPITHELIAL CELLS; EPITHELIAL-MESENCHYMAL TRANSITION; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; NEOPLASMS; RAC1 GTP-BINDING PROTEIN; RECEPTOR, EPIDERMAL GROWTH FACTOR; SIGNAL TRANSDUCTION; VIMENTIN; VINCULIN;

EID: 84869752511     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0049766     Document Type: Article

References (48)

1. Grooteclaes M, Deveraux Q, Hildebrand J, Zhang Q, Goodman RH, et al. (2003) C-terminal-binding protein corepresses epithelial and proapoptotic gene expression programs. Proc Natl Acad Sci U S A 100: 4568-4573.
2. Yu Y, Davicioni E, Triche TJ, Merlino G, (2006) The homeoprotein six1 transcriptionally activates multiple protumorigenic genes but requires ezrin to promote metastasis. Cancer Res 66: 1982-1989.
3. Chambers AF, Groom AC, MacDonald IC, (2002) Dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer 2: 563-572.
4. Condeelis J, Segall JE, (2003) Intravital imaging of cell movement in tumours. Nat Rev Cancer 3: 921-930.
5. Wyckoff J, Wang W, Lin EY, Wang Y, Pixley F, et al. (2004) A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res 64: 7022-7029.
6. Chaffer CL, Thompson EW, Williams ED, (2007) Mesenchymal to epithelial transition in development and disease. Cells Tissues Organs 185: 7-19.
7. Sanz-Moreno V, Gadea G, Ahn J, Paterson H, Marra P, et al. (2008) Rac activation and inactivation control plasticity of tumor cell movement. Cell 135: 510-523.
8. Das S, Becker BN, Hoffmann FM, Mertz JE, (2009) Complete reversal of epithelial to mesenchymal transition requires inhibition of both ZEB expression and the Rho pathway. BMC Cell Biol 10: 94.
9. Thiery JP, (2002) Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2: 442-454.
10. Radisky DC, (2005) Epithelial-mesenchymal transition. J Cell Sci 118: 4325-4326.
11. Behrens J, Mareel MM, Van Roy FM, Birchmeier W, (1989) Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion. J Cell Biol 108: 2435-2447.
12. Park JE, Tan HS, Datta A, Lai RC, Zhang H, et al. (2010) Hypoxia modulates tumor microenvironment to enhance angiogenic and metastastic potential by secretion of proteins and exosomes. Mol Cell Proteomics.
13. Folkman J, (1990) What is the evidence that tumors are angiogenesis dependent? J Natl Cancer Inst 82: 4-6.
14. Brown JM, (1999) The hypoxic cell: a target for selective cancer therapy-eighteenth Bruce F. Cain Memorial Award lecture. Cancer Res 59: 5863-5870.
15. Cho WK, Seong YR, Lee YH, Kim MJ, Hwang KS, et al. (2004) Oncolytic effects of adenovirus mutant capable of replicating in hypoxic and normoxic regions of solid tumor. Mol Ther 10: 938-949.
16. Yan W, Fu Y, Tian D, Liao J, Liu M, et al. (2009) PI3 kinase/Akt signaling mediates epithelial-mesenchymal transition in hypoxic hepatocellular carcinoma cells. Biochem Biophys Res Commun 382: 631-636.
17. Hockel M, Vaupel P, (2001) Tumor hypoxia: definitions and current clinical, biologic, and molecular aspects. J Natl Cancer Inst 93: 266-276.
18. Chan DA, Krieg AJ, Turcotte S, Giaccia AJ, (2007) HIF gene expression in cancer therapy. Methods Enzymol 435: 323-345.
19. Ksiazek K, Mikula-Pietrasik J, Korybalska K, Dworacki G, Jorres A, et al. (2009) Senescent peritoneal mesothelial cells promote ovarian cancer cell adhesion: the role of oxidative stress-induced fibronectin. Am J Pathol 174: 1230-1240.
20. Obrink B, (1986) Epithelial cell adhesion molecules. Exp Cell Res 163: 1-21.
21. Knudsen KA, Soler AP, Johnson KR, Wheelock MJ, (1995) Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin. J Cell Biol 130: 67-77.
22. Simpson KJ, Selfors LM, Bui J, Reynolds A, Leake D, et al. (2008) Identification of genes that regulate epithelial cell migration using an siRNA screening approach. Nat Cell Biol 10: 1027-1038.
23. Ridley AJ, (2006) Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol 16: 522-529.
24. Steffen A, Rottner K, Ehinger J, Innocenti M, Scita G, et al. (2004) Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation. Embo J 23: 749-759.
25. Misra A, Lim RP, Wu Z, Thanabalu T, (2007) N-WASP plays a critical role in fibroblast adhesion and spreading. Biochem Biophys Res Commun 364: 908-912.
26. Alvarez CE, Sutcliffe JG, Thomas EA, (2002) Novel isoform of insulin receptor substrate p53/p58 is generated by alternative splicing in the CRIB/SH3-binding region. J Biol Chem 277: 24728-24734.
27. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, et al. (2003) Cell migration: integrating signals from front to back. Science 302: 1704-1709.
28. Salomon DS, Brandt R, Ciardiello F, Normanno N, (1995) Epidermal growth factor-related peptides and their receptors in human malignancies. Crit Rev Oncol Hematol 19: 183-232.
29. Takeuchi K, Ito F, (2010) EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors. Febs J 277: 316-326.
30. Merlino GT, Xu YH, Ishii S, Clark AJ, Semba K, et al. (1984) Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells. Science 224: 417-419.
31. Franovic A, Gunaratnam L, Smith K, Robert I, Patten D, et al. (2007) Translational up-regulation of the EGFR by tumor hypoxia provides a nonmutational explanation for its overexpression in human cancer. Proc Natl Acad Sci U S A 104: 13092-13097.
32. Thomson S, Buck E, Petti F, Griffin G, Brown E, et al. (2005) Epithelial to mesenchymal transition is a determinant of sensitivity of non-small-cell lung carcinoma cell lines and xenografts to epidermal growth factor receptor inhibition. Cancer Res 65: 9455-9462.
33. Tomasevic N, Jia Z, Russell A, Fujii T, Hartman JJ, et al. (2007) Differential regulation of WASP and N-WASP by Cdc42, Rac1, Nck, and PI(4,5)P2. Biochemistry 46: 3494-3502.
34. Goldstein NI, Prewett M, Zuklys K, Rockwell P, Mendelsohn J, (1995) Biological efficacy of a chimeric antibody to the epidermal growth factor receptor in a human tumor xenograft model. Clin Cancer Res 1: 1311-1318.
35. Pantel K, Brakenhoff RH, (2004) Dissecting the metastatic cascade. Nat Rev Cancer 4: 448-456.
36. Grunert S, Jechlinger M, Beug H, (2003) Diverse cellular and molecular mechanisms contribute to epithelial plasticity and metastasis. Nat Rev Mol Cell Biol 4: 657-665.
37. Rastaldi MP, Ferrario F, Giardino L, Dell'Antonio G, Grillo C, et al. (2002) Epithelial-mesenchymal transition of tubular epithelial cells in human renal biopsies. Kidney Int 62: 137-146.
38. Cano A, Perez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, et al. (2000) The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol 2: 76-83.
39. O'Reilly MS, Holmgren L, Chen C, Folkman J, (1996) Angiostatin induces and sustains dormancy of human primary tumors in mice. Nat Med 2: 689-692.
40. Zhou J, Schmid T, Schnitzer S, Brune B, (2006) Tumor hypoxia and cancer progression. Cancer Lett 237: 10-21.
41. Saunders RM, Holt MR, Jennings L, Sutton DH, Barsukov IL, et al. (2006) Role of vinculin in regulating focal adhesion turnover. Eur J Cell Biol 85: 487-500.
42. Azuma K, Tanaka M, Uekita T, Inoue S, Yokota J, et al. (2005) Tyrosine phosphorylation of paxillin affects the metastatic potential of human osteosarcoma. Oncogene 24: 4754-4764.
43. Sturge J, Hamelin J, Jones GE, (2002) N-WASP activation by a beta1-integrin-dependent mechanism supports PI3K-independent chemotaxis stimulated by urokinase-type plasminogen activator. J Cell Sci 115: 699-711.
44. Kawamoto T, Sato JD, Le A, Polikoff J, Sato GH, et al. (1983) Growth stimulation of A431 cells by epidermal growth factor: identification of high-affinity receptors for epidermal growth factor by an anti-receptor monoclonal antibody. Proc Natl Acad Sci U S A 80: 1337-1341.
45. Konger RL, Chan TC, (1993) Epidermal growth factor induces terminal differentiation in human epidermoid carcinoma cells. J Cell Physiol 156: 515-521.
46. Xie H, Pallero MA, Gupta K, Chang P, Ware MF, et al. (1998) EGF receptor regulation of cell motility: EGF induces disassembly of focal adhesions independently of the motility-associated PLCgamma signaling pathway. J Cell Sci 111 (Pt 5): 615-624.
47. Wells A, Gupta K, Chang P, Swindle S, Glading A, et al. (1998) Epidermal growth factor receptor-mediated motility in fibroblasts. Microsc Res Tech 43: 395-411.
48. Murillo MM, del Castillo G, Sanchez A, Fernandez M, Fabregat I, (2005) Involvement of EGF receptor and c-Src in the survival signals induced by TGF-beta1 in hepatocytes. Oncogene 24: 4580-4587.