Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle

European Journal of Applied Physiology

Volumn 112, Issue 11, 2012, Pages 3905-3911

  Guimarães Ferreira, Lucas ^a,b   Pinheiro, Carlos Hermano J ^a   Gerlinger Romero, Frederico ^a   Vitzel, Kaio F ^a   Nachbar, Renato T ^a   Curi, Rui ^a   Nunes, Maria Tereza ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF ESPÍRITO SANTO   (Brazil)

Author keywords

Antioxidant enzymes; Creatine; Dietary supplementation; Reactive oxygen species; Skeletal muscle

Indexed keywords

EID: 84869206898     PISSN: 14396319     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00421-012-2378-9     Document Type: Article

References (34)

1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121-126
2. Aoki MS, Lima WP, Miyabara EH, Gouveia CH, Moriscot AS (2004) Deleterious effects of immobilization upon rat skeletal muscle: role of creatine supplementation. Clin Nutr 23:1176-1183
3. Azzi A, Montecucco C, Richter C (1975) The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochem Biophys Res Commun 65:597-603
4. Bassit RA, Pinheiro CH, Vitzel KF, Sproesser AJ, Silveira LR, Curi R (2010) Effect of short-term creatine supplementation on markers of skeletal muscle damage after strenuous contractile activity. Eur J Appl Physiol 108:945-955
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
6. Casey A, Constantin-Teodosiu D, Howell S, Hultman E, Greenhaff PL (1996) Creatine ingestion favorably affects performance and muscle metabolism during maximal exercise in humans. Am J Physiol 271:E31-E37
7. Deldicque L, Louis M, Theisen D, Nielens H, Dehoux M, Thissen JP, Rennie MJ, Francaux M (2005) Increased IGF mRNA in human skeletal muscle after creatine supplementation. Med Sci Sports Exerc 37:731-736
8. Droge W (2002) Free radicals in the physiological control of cell function. Physiol Rev 82:47-95
9. Feldman EB (1999) Creatine: a dietary supplement and ergogenic aid. Nutr Rev 57:45-50
10. Flohé L, Gunzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105:114-121
11. Fontaine MA, Geddes JW, Banks A, Butterfield DA (2000) Effect of exogenous and endogenous antioxidants on 3-nitropionic acidinduced in vivo oxidative stress and striatal lesions: insights into Huntington's disease. J Neurochem 75:1709-1715
12. Gerlinger-Romero F, Guimaraes-Ferreira L, Giannocco G, Nunes MT (2011) Chronic supplementation of beta-hydroxy-beta methylbutyrate (HMb) increases the activity of the GH/IGF-I axis and induces hyperinsulinemia in rats. Growth Horm IGF Res 21:57-62
13. Greenhaff Pl (1997) The nutritional biochemistry of creatine. J Nutr Biochem 8:610-618
14. Greenhaff PL, Bodin K, Soderlund K, Hultman E (1994) Effect of oral creatine supplementation on skeletal muscle phosphocreatine resynthesis. Am J Physiol 266:E725-E730
15. Kushmerick MJ, Moerland TS, Wiseman RW (1992) Mammalian skeletal muscle fibers distinguished by contents of phosphocreatine, ATP, and Pi. Proc Natl Acad Sci USA 89:7521-7525
16. Lawler JM, Barnes WS, Wu G, Song W, Demaree S (2002) Direct antioxidant properties of creatine. Biochem Biophys Res Commun 290:47-52
17. Lecour S, Baouali AB, Maupoil V, Chahine R, Abadie C, Javouhey-Donzel A, Rochette L, Nadeau R (1998) Demonstration of the production of oxygen-centered free radicals during electrolysis using E. S. R. spintrapping techniques: effects on cardiac function in the isolated rat heart. Free Radic Biol Med 24:573-579
18. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using realtime quantitative PCR and the 2(-delta delta C(T)) method. Methods 25:402-408
19. Matthews RT, Yang L, Jenkins BG, Ferrante RJ, Rosen BR, Kaddurah-Daouk R, Beal MF (1998) Neuroprotective effects of creatine and cyclocreatine in animal models of Huntington's disease. J Neurosci 18:156-163
20. McKenna MJ, Morton J, Selig SE, Snow RJ (1999) Creatine supplementation increases muscle total creatine but not maximal intermittent exercise performance. J Appl Physiol 87:2244-2252
21. Moylan JS, Reid MB (2007) Oxidative stress, chronic disease, and muscle wasting. Muscle Nerve 35:411-429
22. Oh-Ishi S, Kizaki T, Yamashita H, Nagata N, Suzuki K, Taniguchi N, Ohno H (1995) Alterations of superoxide dismutase iso-enzyme activity, content, and mRNA expression with aging in rat skeletal muscle. Mech Ageing Dev 84:65-76
23. Olsen S, Aagaard P, Kadi F, Tufekovic G, Verney J, Olesen JL, Suetta C, Kjaer M (2006) Creatine supplementation augments the increase in satellite cell and myonuclei number in human skeletal muscle induced by strength training. J Physiol 573:525-534
24. Op 't Eijnde B, Richter EA, Henquin JC, Kiens B, Hespel P (2001) Effect of creatine supplementation on creatine and glycogen content in rat skeletal muscle. Acta Physiol Scand 171:169-176
25. Persky AM, Brazeau GA, Hochhaus G (2003) Pharmacokinetics of the dietary supplement creatine. Clin Pharmacokinet 42:557-574
26. Petrofsky JS, Fitch CD (1980) Contractile characteristics of skeletal muscles depleted of phosphocreatine. Pflugers Arch 384:123-129
27. Pinheiro CH, Silveira LR, Nachbar RT, Vitzel KF, Curi R (2010) Regulation of glycolysis and expression of glucose metabolismrelated genes by reactive oxygen species in contracting skeletal muscle cells. Free Radic Biol Med 48:953-960
28. Pinheiro CH, Vitzel KF, Curi R (2012) Effect of N-acetylcysteine on markers of skeletal muscle injury after fatiguing contractile activity. Scand J Med Sci Sports 22:24-33
29. Rhee SG, Chang TS, Jeong W, Kang D (2010) Methods for detection and measurement of hydrogen peroxide inside and outside of cells. Mol Cells 29:539-549
30. Sestili P, Martinelli C, Bravi G, Piccoli G, Curci R, Battistelli M, Falcieri E, Agostini D, Gioacchini AM, Stocchi V (2006) Creatine supplementation affords cytoprotection in oxidatively injured cultured mammalian cells via direct antioxidant activity. Free Radic Biol Med 40:837-849
31. Stricker PR (1998) Other ergogenic agents. Clin Sports Med 17:283-297
32. 2 . Free Radic Biol Med 1:43-49
33. Wyss M, Kaddurah-Daouk R (2000) Creatine and creatinine metabolism. Physiol Rev 80:1107-1213
34. Zhou M, Diwu Z, Panchuk-Voloshina N, Haugland RP (1997) A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Anal Biochem 253:162-168