Analysis by confocal laser scanning microscopy of the MDPB bactericidal effect on S. mutans biofilm CLSM analysis of MDPB bactericidal effect on biofilm

Journal of Applied Oral Science

Volumn 20, Issue 5, 2012, Pages 568-575

  de Carvalho, Fabíola Galbiatti ^a   Puppin Rontani, Regina Maria ^b   de Fúcio, Suzana Beatriz Portugal ^b   de Cássia Negrini, Thais ^b   Carlo, Hugo Lemes ^c   Garcia Godoy, Franklin ^d  

^a FEDERAL UNIVERSITY OF CAMPINA GRANDE   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

^c Universidade Federal da Paraiba Centro de Ciencias da Saude Faculdade de Medicina   (Brazil)

^d UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

Biofilms; Confocal microscopy; Dentin bonding agents; Streptococcus mutans

Indexed keywords

EID: 84869198434     PISSN: 16787757     EISSN: 16787765     Source Type: Journal    
DOI: 10.1590/S1678-77572012000500013     Document Type: Article

References (23)

1. Beckloff N, Laube D, Castro T, Furgang D, Park S, Perlin D, et al. Activity of an antimicrobial peptide mimetic against planktonic and biofilm cultures of oral pathogens. Antimicrob Agents Chemother. 2007;51:4125-32.
2. Berney M, Hammes F, Bosshard F, Weilenmann H, Egli T. Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight kit in combination with flow cytometry. Appl Environ Microbiol. 2007;73:3283-90.
3. Carvalho FG, Fucio SB, Sinhoreti MA, Correr-Sobrinho L, Puppin-Rontani RM. Confocal laser scanning microscopic analysis of the depth of dentin caries-like lesions in primary and permanent teeth. Braz Dent J. 2008;19:139-44.
4. Chambless JD, Hunt SM, Stewart PS. A three-dimensional computer model of four hypothetical mechanisms protecting biofilms from antimicrobials. Appl Environ Microbiol. 2006;72:2005-13.
5. Fúcio SBP, Puppin-Rontani RM, Carvalho FG, Mattos-Graner RO, Correr-Sobrinho L, Garcia-Godoy F. Analyses of biofilms accumulated on dental restorative materials. Am J Dent. 2009;22:131-6.
6. Gondim JO, Duque C, Hebling J, Giro EM. Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria. J Dent. 2008;36:241-8.
7. Imazato S, Kinomoto Y, Tarumi H, Torii M, Russell RRB, McCabe JF. Incorporation of antibacterial monomer MDPB into dentin primer. J Dent Res. 1997;76:768-72.
8. Imazato S, Kuramoto A, Takahashi Y, Ebisu S, Peters MC. In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. Dent Mater. 2006;22:527-32.
9. Imazato S, Ohmori K, Russell RR, McCabe JF, Momoi Y, Maeda N. Determination of bactericidal activity of antibacterial monomer MDPB by a viability staining method. Dent Mater J. 2008;27:145-8.
10. Izutani N, Imazato S, Nakajo K, Takahashi N, Takahashi Y, Ebisu S, et al. Effects of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB) on bacterial viability and metabolism. Eur J Oral Sci. 2011;119:175-81.
11. Korkmaz Y, Ozalp M, Attar N. Comparison of the antibacterial activity of different self-etching primers and adhesives. J Contemp Dent Pract. 2008;9:57-64.
12. Lima GQ, Oliveira EG, Souza JI, Monteiro Neto V. Comparison of the efficiency of chemomechanical and mechanical methods of caries removal in the reduction of Streptococcus mutans and Lactobacillus spp in carious dentine of primary teeth. J Appl Oral Sci. 2005;13:399-405.
13. Mikami Y, Suzuki N, Takahashi T, Otsuka K, Tsuda H. Bacitracin upregulates mbrAB transcription via mbrCD to confer bacitracin resistance in Streptococcus mutans. J Pharmacol Sci. 2011;117:204-7.
14. Neelakantan P, Rao CVS, Indramohan J. Bacteriology of deep carious lesions underneath amalgam restorations with different pulp-capping materials: an in vivo analysis. J Appl Oral Sci. 2012;20:139-45.
15. Rolland SL, McCabe JF, Robinson C, Walls AW. In vitro biofilm formation on the surface of resin-based dentine adhesives. Eur J Oral Sci. 2006;114: 243-9.
16. Silva NR, Calamia CS, Coelho PG, Carrilho MR, Carvalho RM, Caufield P, et al. Effect of 2% iodine disinfecting solution on bond strength to dentin. J Appl Oral Sci. 2006;14:399-404.
17. Stewart PS. Diffusion in biofilms. J Bacteriol. 2003;185:1485-91.
18. Susin AH, Alves LS, Melo GP, Lenzi TL. Comparative scanning electron microscopic study of the effect of different dental conditioners on dentin micromorphology. J Appl Oral Sci. 2008;16:100-5.
19. Ten Cate JM. Biofilms, a new approach to the microbiology of dental plaque. Odontology. 2006;94:1-9.
20. Türkün M, Türkün LS, Ergücü Z, Ateş M. Is an antibacterial adhesive system more effective than cavity disinfectants? Am J Dent. 2006;19:166-70.
21. Wambier DS, Santos FA, Guedes-Pinto AC, Jaeger RG, Simionato MRL. Ultrastructral and microbiological analysis of dentin layers affected by caries lesions in primary molars treated by minimal intervention. Ped Dent. 2007;29:228-34.
22. Wood SR, Kirkham J, Marsh PD, Shore RC, Nattress B, Robinson C. Architecture of intact natural human plaque biofilms studied by confocal laser scanning microscopy. J Dent Res. 2000;79:21-7.
23. Yoshikawa K, Clark DT, Brailsford SR, Beighton D, Watson TF, Imazato S, et al. The effect of antibacterial MDPB monomer on the growth of organisms associated with root caries. Dent Mater J. 2007;26:388-92.