Fast test for assessing the susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin by real-time PCR

Memorias do Instituto Oswaldo Cruz

Volumn 107, Issue 7, 2012, Pages 903-908

  Gonçalves, Maria Gisele ^a   Fukasawa, Lucila Okuyama ^a   Oliveira, Rosangela Siqueira ^a   Salgado, Maristela Marques ^a   Harrison, Lee H ^b   Shutt, Kathleen A ^b   Sacchi, Claudio Tavares ^a  

^a INSTITUTO ADOLFO LUTZ   (Brazil)

^b UNIVERSITY OF PITTSBURGH GRADUATE SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

Drug resistance; INH; Mycobacterium tuberculosis; PCR; RIF; RT

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; CATALASE; INHA PROTEIN, MYCOBACTERIUM; ISONIAZID; KATG PROTEIN, MYCOBACTERIUM TUBERCULOSIS; OXIDOREDUCTASE; RIFAMPICIN; RPOB PROTEIN, MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSTATIC AGENT;

ANTIBIOTIC RESISTANCE; ARTICLE; DRUG EFFECT; GENETICS; HUMAN; METHODOLOGY; MICROBIAL SENSITIVITY TEST; MUTATION; MYCOBACTERIUM TUBERCULOSIS; REAL TIME POLYMERASE CHAIN REACTION;

ANTITUBERCULAR AGENTS; BACTERIAL PROTEINS; CATALASE; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; HUMANS; ISONIAZID; MICROBIAL SENSITIVITY TESTS; MUTATION; MYCOBACTERIUM TUBERCULOSIS; OXIDOREDUCTASES; REAL-TIME POLYMERASE CHAIN REACTION; RIFAMPIN;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS;

EID: 84869190831     PISSN: 00740276     EISSN: 16788060     Source Type: Journal    
DOI: 10.1590/S0074-02762012000700011     Document Type: Article

References (34)

1. Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, Allen J, Tahirli R, Blakemore R, Rustomjee R, Milovic A, Jones M, O'Brien SM, Persing DH, Ruesch-Gerdes S, Gotuzzo E, Rodriguies C, Alland D, Perkins MD 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med 363: 1005-1015.
2. Cardoso RF, Cooksey RC, Morlok GP, Barco P, Cecon L, Forestiero F, Leite CQ, Sato DN, de Lourdes Shikama M, Mamizuka EM, Hirata MH 2004. Screening and characterization of mutations in isoniazid-resistent Mycobacterium tuberculosis isolates obtained in Brazil. Antimicrob Agents Chemother 48: 3373-3381.
3. Clemente WT, Lima SSS, Palaci M, Silva MSN, Rodrigues VFS, Costa ERD, Possuelo L, Cafrune PI, Ribeiro FK, Gomes HM, Serufo JC 2008. Phenotypic and genotypic characterization of drug-resistant Mycobacterium tuberculosis strains. Diag Microbiol Infec Dis 62: 199-204.
4. de Miranda SS, Kritski AL, Filliol I, Mabilat C, Panteix G, Drouet E 2001. Mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains isolated in Brazil and France. Mem Inst Oswaldo Cruz 96: 247-250.
5. El-Hajj HH, Marras SAE, Tyagi S, Kramer FR, Alland D 2001. Detection of rifampin resistance in Mycobacterium tuberculosis in a single tube with molecular beacons. J Clin Microbiol 39: 4131-4137.
6. Garcia de Viedma D 2003. Rapid detection of resistance in Mycobacterium tuberculosis: a review discussing molecular approaches. Clin Microbiol Infect 9: 349-359.
7. Garcia de Viedma D, Infantes MSD, Lasala F, Chaves F, Alcalá L, Bouza E 2002. New real-time PCR able to detect in a single tube multiple rifampin resistance mutations and high-level isoniazid resistance mutations in Mycobacterium tuberculosis. J Clin Microbiol 40: 988-995.
8. Helb D, Jones M, Story E, Boehme C, Wallace E, Ho K 2010. Rapid detection of Mycobacterium tuberculosis and rifampin-resistance using on-demand, near-patient technology. J Clin Microbiol 1: 229-237.
9. Hofling CC, Pavan EM, Giampaglia CMS, Ferrazoli L, Aily DCB, de Albuquerque DM, Ramos MC 2005. Prevalence of katG Ser315 substitution and rpoB mutations in isoniazid-resistant Mycobacterium tuberculosis isolates from Brazil. Int J Tuberc Lung Dis 9: 87-89.
10. Kapur V, Li LL, Iordanescu S, Hamrick MR, Wanger A, Kreiswirth BN, Musser JM 1994. Characterization by automatic DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York city and Texas. J Clin Microbiol 32: 1095-1098.
11. Kocagoz T, Saribas Z, Alp A 2005. Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR. J Clin Microbiol 43: 6015-6019.
12. Lawn SD, Nicol MP 2011. Xpert® MTB/RIF assay: development, evaluation and implementation of a new rapid molecular diagnostic for tuberculosis and rifampicin resistance. Review. Future Microbiol 6: 1067-1082.
13. Marín M, Viedma DG, Ruíz-Serrano MJ, Bouza E 2004. Rapid direct detection of multiple rifampin and isoniazid resistance mutations in Mycobacterium tuberculosis in respiratory samples by real-time PCR. Antimicrob Agents Chemother 48: 4293-4300.
14. MS/SVS-Ministério da Saúde/Secretaria de Vigilância em Saúde 2009. Informe eletrônico de tuberculose. Boletim epidemiológico 2. Available from: portal. saude. gov. br/portal/arquivos/pdf/boletim_tb_julho09. pdf.
15. MS/SVS-Ministério da Saúde/Secretaria de Vigilância em Saúde 2010. Programa Nacional de Controle da Tuberculose 2010. Ma-nual de recomendações para o controle da tuberculose no Brasil. Available from: portal. saude. gov. br/portal/arquivos/pdf/manualderecomendacoescontroletbnovo. pdf.
16. MS/SVS/DVE-Ministério da Saúde/Secretaria de Vigilância em Saúde/Departamento de Vigilância Epidemiológica 2008 Ma-nual nacional de vigilância laboratorial da tuberculose e outras micobactérias, 1st ed., MS/SVS/DVE, Brasília, p. 323-348.
17. Piatek AS, Telenti A, Murray MR, El-Hajj H, Jacobs Jr WR, Kramer FR, Alland D 2000. Genotypic analysis of Mycobacterium tuberculosis in two distinct populations using molecular beacons: implications for rapid susceptibility testing. Antimicrob Agents Chemother 44: 103-110.
18. Piatek AS, Tyagi S, Pol AC, Telenti A, Miller LP, Kramer FR, Aland D 1998. Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis. Nat Biotechnol 16: 359-363.
19. Riska PF, Jacobs Jr WR, Alland D 2000. Molecular determinants of drug resistance in tuberculosis. Int J Tuberc Lung Dis 4 (Suppl.): S4-S10.
20. Rossetti MLR, Valim ARM, Silva MSN, Rodrigues VS 2002. Tuberculose resistente: revisão molecular. Rev Saude Publica 36: 525-532.
21. Ruiz M, Torres MJ, Llanos AC, Arroyo A, Palomares JC, Aznar J 2004. Direct detection of rifampin and isoniazid-resistant Mycobacterium tuberculosis in auramine-rhodamine-positive sputum specimens by real-time PCR. J Clin Microbiol 42: 1585-1589.
22. SESSP/SVE-Secretaria de Estado da Saúde de São Paulo/Centro de Vigilância Epidemiológica 2006. Tuberculose no estado de São Paulo. Indicadores de morbimortalidade e indicadores de desempenho. Boletim Epidemiológico Paulista 3 (Suppl. 4). Available from: ftp://ftp.cve.saude.sp.gov.tp.tb.br/doctec/outros/BEPA TBSUPLE4V3.pdf.
23. Telenti A, Honore N, Bernasconi C, March J, Ortega A, Heym B, Takiff HE, Cole ST 1997. Genotypic assessment of isoniazid and rifampin resistance in Mycobacterium tuberculosis: a blind study at reference laboratorial level. J Clin Microbiol 35: 719-723.
24. Telenti A, Imboden P, Marchesi F, Matter L, Schopfer K, Bodmer T, Lowrie D, Cole S, Colston MJ, Cole S 1993. Detection of rifampicin resistance mutations in Mycobacterium tuberculosis. Lancet 341: 647-650.
25. Torres JM, Criado A, Ruiz M, Llanos AC, Palomares JC, Aznar J 2003. Improved real-time PCR for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates. Diag Microbiol Infec Dis 45: 207-212.
26. Torres MJ, Criado A, Palomares JC, Aznar J 2000. Use of real-time PCR and fluorimetry for rapid detection of rifampin resistance-associated mutations in Mycobacterium tuberculosis. J Clin Microbiol 38: 3194-3199.
27. Valim ARM, Rossetti MLR, Ribeiro MO, Zaha A 2000. Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis isolates from Brasil. J Clin Microbiol 38: 3119-3122.
28. van Doorn HR, Class EC, Templeton KE, van der Zanden AGM, Vije AK, de Jong MD, Dankert J, Kuijper EJ 2003. Detection of a point mutation associated with high-level isoniazid resistance in Mycobacterium tuberculosis by real-time PCR technology with 3'-minor groove binder-DNA probes. J Clin Microbiol 41: 4630-4635.
29. van Soolingen D, Hass PEW, van Door HR, Kuijper E, Rinder H, Borgdorff MW 2000. Mutations at amino acid position 315 of the katG gene area associated with high-level resistance to isoniazid, other drug resistance, and successful transmission of Mycobacterium tuberculosis in the Netherlands. Concise communication. J Infec Dis 182: 1788-1790.
30. Wada T, Maeda S, Tamaru A, Imai S, Hase A, Kobayashi K 2004. Dual-probe assay for rapid detection of drug-resistant Mycobacterium tuberculosis by real-time PCR. J Clin Microbiol 42: 5277-5285.
31. WHO-World Health Organization 2009a. Global tuberculosis control report. Available from: who. int/tb/publications/2009/tbfactsheet_2009update_one_page. pdf.
32. WHO-World Health Organization 2009b. Global tuberculosis control. A short update to the 2009 report. Available from: who. int/tb/publications/global_report/2009/update/tb_9. pdf.
33. Yesilkaya H, Meacci F, Niemann S, Hillemann D, Rüsh-Gerdes S, Barer MR, Andrew PW, Oggioni MR, Long Drug Study Group 2006. Evaluation of molecular-beacon, TaqMan and fluorescence resonance energy transfer probes for detection of antibiotic resistance-conferring single nucleotide polymorphisms in mixed Mycobacterium tuberculosis DNA extracts. J Clin Microbiol 44: 3826-3829.
34. Zeka ZN, Tasbakan S, Cavusoglu C 2011. Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens. J Clin Microbiol 49: 4138-4414.