Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens

Journal of Clinical Microbiology

Volumn 49, Issue 12, 2011, Pages 4138-4141

  Zeka, Arzu N ^a   Tasbakan, Sezai ^a   Cavusoglu, Cengiz ^a  

^a EGE UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

ETHAMBUTOL; ISONIAZID; RIFAMPICIN; TUBERCULOSTATIC AGENT;

ADULT; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL COUNT; BACTERIUM CULTURE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; EXTRAPULMONARY TUBERCULOSIS; FEMALE; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LUNG TUBERCULOSIS; MAJOR CLINICAL STUDY; MALE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SMEAR; TUBERCULOSIS;

ADULT; AGED; ANTITUBERCULAR AGENTS; BACTERIOLOGICAL TECHNIQUES; FEMALE; HUMANS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS; RIFAMPIN; SENSITIVITY AND SPECIFICITY; TUBERCULOSIS;

EID: 82455220464     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.05434-11     Document Type: Article

References (19)

1. Anonymous. 2009. Updated guidelines for the use of nucleic acid amplification tests in the diagnosis of tuberculosis. MMWR Morb. Mortal. Wkly. Rep. 58:7-10.
2. Armand, S., P. Vanhuls, G. Delcroix, R. Courcol, and N. Lemaître. 2011. Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J. Clin. Microbiol. 49:1772-1776.
3. Blakemore, R., et al. 2010. Evaluation of the analytical performance of the Xpert MTB/RIF assay. J. Cin Microbiol. 48:2495-2501.
4. Boehme, C. C., et al. 2010. Rapid molecular detection of tuberculosis and rifampin resistance. N. Engl. J. Med. 363:1005-1015.
5. Boehme, C. C., et al. 2011. Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study. Lancet 377:1495-1505.
6. Cavusoglu, C., S. Hilmioglu, S. Guneri, and A. Bilgic. 2002. Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J. Clin. Microbiol. 40:4435-4438. (Pubitemid 35434927)
7. Cirillo, D. M., et al. 2004. Direct rapid diagnosis of rifampicin resistant Mycobacterium tuberculosis infection in clinical samples by line probe assay (INNO LiPA Rif. TB). New Microbiol. 27:221-227. (Pubitemid 40089472)
8. CLSI/NCCLS. 2003. Susceptibility testing of mycobacteria, nocardia, and other aerobic actinomycetes; approved standard. Document M24-A. National Committee for Clinical Laboratory Standards, Wayne, PA.
9. Helb, D., et al. 2010. Rapid detection of Mycobacterium tuberculosis and rifampin resistance by use of on-demand, near-patient technology. J. Clin. Microbiol. 48:229-237.
10. Hillemann, D., M. Weizenegger, T. Kubica, E. Richter, and S. Niemann. 2005. Use of the Genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis complex isolates. J. Clin. Microbiol. 43:3699-3703. (Pubitemid 41129648)
11. Huang, W. L., H. Y. Chen, Y. M. Kuo, and R. Jou. 2009. Performance assessment of the GenoType MTBDRplus test and DNA sequencing in detection of multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 47:2520-2524.
12. Kent, P. T., and G. P. Kubica. 1985. Public health mycobacteriology. A guide for a level III laboratory. Centers for Disease Control, Atlanta, GA.
13. Makinen, J., H. J. Marttila, M. Marjamaki, M. K. Viljanen, and H. Soini. 2006. Comparison of two commercially available DNA line probe assays for detection of multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 44:350-352. (Pubitemid 43222221)
14. Marlowe, E. M., et al. 2011. Evaluation of the Cepheid Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis complex in respiratory specimens. J. Clin. Microbiol. 49:1621-1623.
15. Mani, C., N. Selvakumar, S. Narayanan, and P. R. Narayanan. 2001. Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India. J. Clin. Microbiol. 39:2987-2990. (Pubitemid 32744028)
16. Moure, R., et al. 2011. Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method. J. Clin. Microbiol. 49:1137-1139.
17. Raja, S., et al. 2005. Technology for automated, rapid, and quantitative PCR or reverse transcription-PCR clinical testing. Clin. Chem. 51:882-890. (Pubitemid 40577926)
18. Telenti, A., et al. 1993. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet 341:647-650. (Pubitemid 23083244)
19. Tuberculosis Service. 2009. Tuberculosis report in Turkey. Ministry of Health, Ankara, Turkey.