Evidence for pH-dependent protease activity in the adeno-associated virus capsid

Journal of Virology

Volumn 86, Issue 21, 2012, Pages 11877-11885

  Salganik, Maxim ^a   Venkatakrishnan, Balasubramanian ^a   Bennett, Antonette ^a   Lins, Bridget ^a   Yarbrough, Joseph ^a   Muzyczka, Nicholas ^a   Agbandje McKenna, Mavis ^a   McKenna, Robert ^a  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CAPSID PROTEIN; PROTEINASE;

ADENO ASSOCIATED VIRUS; ARTICLE; AUTOLYSIS; CONTAMINATION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; IN VITRO STUDY; INCUBATION TIME; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN PURIFICATION; SEROTYPE; VIRUS CAPSID;

CAPSID; CAPSID PROTEINS; DEPENDOVIRUS; HYDROGEN-ION CONCENTRATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PEPTIDE HYDROLASES; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY;

ADENO-ASSOCIATED VIRUS; MIRIDAE;

EID: 84869060596     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.01717-12     Document Type: Article

References (23)

1. Bartlett JS, Wilcher R, Samulski RJ. 2000. Infectious entry pathway of adeno-associated virus and adeno-associated virus vectors. J. Virol. 74:2777-2785.
2. Berns KI, Parrish CR. 2007. Parvoviridae, p 2437-2477. In Knipe DM, HowleyPM(ed), Fields virology, 5th ed. Lippincott Williams and Wilkins, New York, NY.
3. Douar AM, Poulard K, Stockholm D, Danos O. 2001. Intracellular trafficking of adeno-associated virus vectors: routing to the late endosomal compartment and proteasome degradation. J. Virol. 75:1824-1833.
4. Farr GA, Cotmore SF, Tattersall P. 2006. VP2 cleavage and the leucine ring at the base of the fivefold cylinder control pH-dependent externalization of both the VP1 N terminus and the genome of minute virus of mice. J. Virol. 80:161-171.
5. Girod A, et al. 2002. The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity. J. Gen. Virol. 83:973-978.
6. Grieger JC, Johnson JS, Gurda-Whitaker B, Agbandje-McKenna M, Samulski RJ. 2007. Surface-exposed adeno-associated virus Vp1-NLS capsid fusion protein rescues infectivity of noninfectious wild-type Vp2/Vp3 and Vp3-only capsids but not that of fivefold pore mutant virions. J. Virol. 81:7833-7843.
7. Hansen J, Qing K, Srivastava A. 2001. Adeno-associated virus type 2-mediated gene transfer: altered endocytic processing enhances transduction efficiency in murine fibroblasts. J. Virol. 75:4080-4090.
8. Johnson JE, Vogt PK. 2010. Cell entry by non-enveloped viruses. Curr. Top. Microbiol. Immunol. 343:v-vii.
9. Keller A, Nesvizhskii AI, Kolker E, Aebersold R. 2002. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal. Chem. 74:5383-5392.
10. Kern A, et al. 2003. Identification of a heparin-binding motif on adenoassociated virus type 2 capsids. J. Virol. 77:11072-11081.
11. Kronenberg S, Bottcher B, von der Lieth CW, Bleker S, Kleinschmidt JA. 2005. A conformational change in the adeno-associated virus type 2 capsid leads to the exposure of hidden VP1 N termini. J. Virol. 79:5296-5303.
12. Lane MD, et al. 2005. Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61:558-561.
13. Lochrie MA, et al. 2006. Mutations on the external surfaces of adenoassociated virus type 2 capsids that affect transduction and neutralization. J. Virol. 80:821-834.
14. Nam HJ, et al. 2011. Structural studies of adeno-associated virus serotype 8 capsid transitions associated with endosomal trafficking. J. Virol. 85: 11791-11799.
15. Nesvizhskii AI, Keller A, Kolker E, Aebersold R. 2003. A statistical model for identifying proteins by tandem mass spectrometry. Anal. Chem. 75: 4646-4658.
16. Odegard AL, et al. 2009. Low endocytic pH and capsid protein autocleavage are critical components of Flock House virus cell entry. J. Virol. 83:8628-8637.
17. Opie SR, Warrington KH, Jr, Agbandje-McKenna M, Zolotukhin S, Muzyczka N. 2003. Identification of amino acid residues in the capsid proteins of adeno-associated virus type 2 that contribute to heparan sulfate proteoglycan binding. J. Virol. 77:6995-7006.
18. Sonntag F, Bleker S, Leuchs B, Fischer R, Kleinschmidt JA. 2006. Adeno-associated virus type 2 capsids with externalized VP1/VP2 trafficking domains are generated prior to passage through the cytoplasm and are maintained until uncoating occurs in the nucleus. J. Virol. 80:11040-11054.
19. Thomas DL, et al. 2009. Scalable recombinant adeno-associated virus production using recombinant herpes simplex virus type 1 coinfection of suspension-adapted mammalian cells. Hum. Gene Ther. 20:861-870.
20. Van Vliet K, Blouin V, Agbandje-McKenna M, Snyder RO. 2006. Proteolytic mapping of the adeno-associated virus capsid. Mol. Ther. 14:809-821.
21. Wobus CE, et al. 2000. Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virol. 74:9281-9293.
22. Wu P, et al. 2000. Mutational analysis of the adeno-associated virus type 2 (AAV2) capsid gene and construction f AAV2 vectors with altered tropism. J. Virol. 74:8635- 8647.
23. Zolotukhin S, et al. 2002. Production and purification of serotype 1, 2, and 5 recombinant adeno-associated viral vectors. Methods 28:158-167.