Using Digital RNA Counting and Flow Cytometry to Compare mRNA with Protein Expression in Acute Leukemias

PLoS ONE

Volumn 7, Issue 11, 2012, Pages

  Fernandez, Paula ^a   Solenthaler, Max ^b   Spertini, Olivier ^c   Quarroz, Stephane ^c   Rovo, Alicia ^d   Lovey, Pierre Yves ^e   Leoncini, Leda ^f   Ruault Jungblut, Sylvie ^g   D'Asaro, Mathilde ^g   Schaad, Olivier ^h   Docquier, Mylène ^h   Descombes, Patrick ^h   Matthes, Thomas ^g  

^a CANTONAL HOSPITAL AARAU   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

^c LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^d UNIVERSITY HOSPITAL BASEL   (Switzerland)

^e Institut Central des Hopitaux Valaisans   (Switzerland)

^f REGIONAL HOSPITAL OF LUGANO   (Switzerland)

^g GENEVA UNIVERSITY HOSPITALS   (Switzerland)

^h UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CD11B ANTIGEN; CD123 ANTIGEN; CD133 ANTIGEN; CD14 ANTIGEN; CD15 ANTIGEN; CD16 ANTIGEN; CD19 ANTIGEN; CD20 ANTIGEN; CD3 ANTIGEN; CD33 ANTIGEN; CD34 ANTIGEN; CD36 ANTIGEN; CD38 ANTIGEN; CD4 ANTIGEN; CD56 ANTIGEN; CD7 ANTIGEN; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN; DNA NUCLEOTIDYLEXOTRANSFERASE; HLA DR ANTIGEN; MESSENGER RNA; MICROSOMAL AMINOPEPTIDASE; MYELOPEROXIDASE; STEM CELL FACTOR RECEPTOR;

ACUTE LEUKEMIA; ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ARTICLE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; DIGITAL RNA COUNTING; FLOW CYTOMETRY; GENE EXPRESSION; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NATURAL KILLER CELL; PROTEIN EXPRESSION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY;

ANTIGENS, CD; BLOOD CELLS; BONE MARROW CELLS; FLOW CYTOMETRY; GENOMICS; HUMANS; IMMUNOPHENOTYPING; LEUKEMIA; PROTEOME; PROTEOMICS; TRANSCRIPTOME;

EID: 84869055286     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0049010     Document Type: Article

References (13)

1. Bandura DR, Baranov VI, Ornatsky OI, Antonov A, Kinach R, et al. (2009) Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry. Anal Chem 81: 6813-6822.
2. Ornatsky O, Bandura D, Baranov V, Nitz M, Winnik MA, et al. (2010) Highly multiparametric analysis by mass cytometry. J Immunol Methods 361: 1-20.
3. Belov L, Mulligan SP, Barber N, Woolfson A, Scott M, et al. (2006) Analysis of human leukaemias and lymphomas using extensive immunophenotypes from an antibody microarray. Br J Haematol 135: 184-197.
4. Barber N, Gez S, Belov L, Mulligan SP, Woolfson A, et al. (2009) Profiling CD antigens on leukaemias with an antibody microarray. FEBS Lett 583: 1785-1791.
5. Alizadeh AA, Eisen MB, Davis RE, Ma C, Lossos IS, et al. (2000) Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 403: 503-511.
6. Haferlach T, Kohlmann A, Wieczorek L, Basso G, Kronnie GT, et al. (2010) Clinical utility of microarray-based gene expression profiling in the diagnosis and subclassification of leukemia: report from the International Microarray Innovations in Leukemia Study Group. J Clin Oncol 28: 2529-2537.
7. Geiss GK, Bumgarner RE, Birditt B, Dahl T, Dowidar N, et al. (2008) Direct multiplexed measurement of gene expression with color-coded probe pairs. Nat Biotechnol 26: 317-325.
8. Beaume M, Hernandez D, Docquier M, Delucinge-Vivier C, Descombes P, et al. (2011) Orientation and expression of methicillin-resistant Staphylococcus aureus small RNAs by direct multiplexed measurements using the nCounter of NanoString technology. J Microbiol Methods 84: 327-334.
9. Payton JE, Grieselhuber NR, Chang LW, Murakami M, Geiss GK, et al. (2009) High throughput digital quantification of mRNA abundance in primary human acute myeloid leukemia samples. J Clin Invest 119: 6; 1714-1726.
10. Malkov VA, Serikawa KA, Balantac N, Watters J, Geiss G, et al. (2009) Multiplexed measurements of gene signatures in different analytes using the Nanostring nCounter Assay System. BMC Res Notes 2: 80-89.
11. Kern W, Kohlmann A, Schoch C, Schnittger S, Haferlach T, (2006) Comparison of mRNA abundance quantified by gene expression profiling and percentage of positive cells using immunophenotyping for diagnostic antigens in acute and chronic leukemias. Cancer 107: 2401-2407.
12. Kulkarni MM (2011) Digital multiplexed gene expression analysis using the NanoString nCounter system. Curr Protoc Mol Biol. Apr; Chapter 25: Unit 25B.10.
13. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: Jun 18; 3(7); Epub 2002 Jun 18.