Construction and characterization of a reporter gene cell line for assessment of human glucocorticoid receptor activation

European Journal of Pharmaceutical Sciences

Volumn 47, Issue 5, 2012, Pages 842-847

  Novotna, Aneta ^a   Pavek, Petr ^b   Dvorak, Zdenek ^a  

^a PALACKÝ UNIVERSITY   (Czech Republic)

^b Charles University in Prague Faculty of Pharmacy   (Czech Republic)

Author keywords

Cell line; Gene reporter assay; Glucocorticoid receptor; Glucocorticoids

Indexed keywords

BECLOMETASONE; BETAMETHASONE; DEXAMETHASONE; GLUCOCORTICOID RECEPTOR; LUCIFERASE; MIFEPRISTONE; PREDNISOLONE;

ARTICLE; CELL LINE; CRYOPRESERVATION; DRUG POTENCY; GENE CONSTRUCT; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; MOLECULAR CLONING; PRIORITY JOURNAL; REPORTER GENE;

GENES, REPORTER; GLUCOCORTICOIDS; HELA CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LUCIFERASES; RECEPTORS, GLUCOCORTICOID; STEROIDS;

EID: 84868696448     PISSN: 09280987     EISSN: 18790720     Source Type: Journal    
DOI: 10.1016/j.ejps.2012.10.003     Document Type: Article

References (9)

1. Z Dvorak, and P Pavek Regulation of drug-metabolizing cytochrome P450 enzymes by glucocorticoids Drug Metab. Rev. 42 4 2010 621 635
2. P.J. Fuller, S.S. Lim-Tio, and F.E. Brennan Kidney Int. Apr. 57 4 2000 1256 1264
3. S.M. Hollenberg, C. Weinberger, and E.S. Ong Primary structure and expression of a functional human glucocorticoid receptor cDNA Nature 318 6047 1985 635 641 (Pubitemid 16172412)
4. D.I. Israel, and R.J. Kaufman Highly inducible expression from vectors containing multiple GRE's in CHO cells overexpressing the glucocorticoid receptor Nucleic Acids Res. 17 12 1989 4589 4604 (Pubitemid 19165032)
5. T. Kino, I. Manoli, S. Kelkar, Y. Wang, Y.A. Su, and G.P. Chrousos Glucocorticoid receptor (GR) beta has intrinsic, GRalpha-independent transcriptional activity Biochem. Biophys. Res. Commun. 381 4 2009 671 675
6. T. Mori, F. Saito, T. Yoshino, H. Takeyama, and T. Matsunaga Reporter gene assay against lipophilic chemicals based on site-specific genomic recombination of a nuclear receptor gene, its response element, and a luciferase reporter gene within a stable HeLa cell line Biotechnol. Bioeng. 99 6 2008 1453 1461 (Pubitemid 351548685)
7. D Sedlak, A Paguio, and P Bartunek panels of steroid receptor luciferase reporter cell lines for compound profiling Comb. Chem. High T. Scr. 14 4 2011 248 266
8. J.M. Wilkinson, S. Hayes, D. Thompson, P. Whitney, and K. Bi Compound profiling using a panel of steroid hormone receptor cell-based assays J. Biomol. Screen. 13 8 2008 755 765 10.1177/1087057108322155
9. V.S. Wilson, K. Bobseine, C.R. Lambright, and L.E. Gray Jr. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists Toxicol. Sci. 66 1 2002 69 81 (Pubitemid 34202030)