Reporter gene assay against lipophilic chemicals based on site-specific genomic recombination of a nuclear receptor gene, its response element and a luciferase reporter gene within a stable HeLa cell line

Biotechnology and Bioengineering

Volumn 99, Issue 6, 2008, Pages 1453-1461

  Mori, Tetsushi ^a   Saito, Fumiyo ^b   Yoshino, Tomoko ^a   Takeyama, Haruko ^a   Matsunaga, Tadashi ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b CHEMICALS EVALUATION AND RESEARCH INSTITUTE   (Japan)

Author keywords

Genomic recombination; Human glucocorticoid receptor; Lipophilic chemicals; Stable cell reporter gene assay

Indexed keywords

BIOSYNTHESIS; CLONING; ENZYME ACTIVITY; SENSITIVITY ANALYSIS;

GENOMIC RECOMBINATION; HUMAN GLUCOCORTICOID RECEPTOR; LIPOPHILIC CHEMICALS; STABLE CELL REPORTER GENE ASSAY;

GENE EXPRESSION;

DEXAMETHASONE; GLUCOCORTICOID; GLUCOCORTICOID RECEPTOR; GLUCOCORTICOID RECEPTOR ANTAGONIST; LUCIFERASE; CELL RECEPTOR;

ARTICLE; CHEMICAL ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; GENETIC RECOMBINATION; GENETIC STABILITY; GENOMICS; HUMAN; HUMAN CELL; LIPOPHILICITY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; REPORTER GENE; SENSITIVITY AND SPECIFICITY; BIOASSAY; DNA RESPONSIVE ELEMENT; DRUG EFFECT; FLUORESCENCE MICROSCOPY; GENETICS; HELA CELL; LIPID METABOLISM; METHODOLOGY;

BIOLOGICAL ASSAY; DEXAMETHASONE; GENES, REPORTER; GLUCOCORTICOIDS; HELA CELLS; HUMANS; LIPID METABOLISM; LUCIFERASES; MICROSCOPY, FLUORESCENCE; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINATION, GENETIC; RESPONSE ELEMENTS;

EID: 41049118497     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21729     Document Type: Article

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