Structural and functional characterization of the nitrite channel NirC from Salmonella typhimurium

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 45, 2012, Pages 18395-18400

  Lü, Wei ^a   Schwarzer, Nikola J ^a   Du, Juan ^a   Gerbig Smentek, Elke ^a   Andrade, Susana L A ^a   Einsle, Oliver ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Ion channels; Planar lipid bilayer; Protein crystallography

Indexed keywords

AQUAPORIN; BACTERIAL PROTEIN; FOCA PROTEIN; FORMIC ACID; NIRC PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CHANNEL GATING; CONDUCTANCE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ELECTROPHYSIOLOGY; LIPID BILAYER; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN STRUCTURE; SALMONELLA TYPHIMURIUM; X RAY CRYSTALLOGRAPHY;

AMINO ACIDS; ANION TRANSPORT PROTEINS; ANIONS; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT; CRYSTALLOGRAPHY, X-RAY; ELECTRIC CONDUCTIVITY; FORMATES; HYDROGEN-ION CONCENTRATION; ION CHANNEL GATING; ION CHANNELS; MODELS, MOLECULAR; NITRITES; PROTEIN SUBUNITS; PROTONS; SALMONELLA TYPHIMURIUM; STRUCTURAL HOMOLOGY, PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; SALMONELLA TYPHIMURIUM;

EID: 84868575935     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1210793109     Document Type: Article

References (35)

1. Einsle O, Kroneck PMH (2004) Structural basis of denitrification. Biol Chem 385(10): 875- 883.
2. Martínez-Espinosa RM, Cole JA, Richardson DJ, Watmough NJ (2011) Enzymology and ecology of the nitrogen cycle. Biochem Soc Trans 39(1):175-178.
3. Gilberthorpe NJ, PooleRK(2008)Nitricoxidehomeostasis in Salmonella typhimurium: Roles of respiratory nitrate reductase and flavohemoglobin. J Biol Chem 283(17):11146-11154.
4. Wink DA, et al. (1991) DNA deaminating ability and genotoxicity of nitric oxide and its progenitors. Science 254(5034):1001-1003. (Pubitemid 21917428)
5. Hibbs JB, Jr., Taintor RR, Vavrin Z, Rachlin EM (1988) Nitric oxide: A cytotoxic activated macrophage effector molecule. Biochem Biophys Res Commun 157(1):87-94. (Pubitemid 19004828)
6. Canfield DE, Glazer AN, Falkowski PG (2010) The evolution and future of Earth's nitrogen cycle. Science 330(6001):192-196.
7. Saier MH, Jr., et al. (1999) Phylogenetic characterization of novel transport protein families revealed by genome analyses. Biochim Biophys Acta 1422(1):1-56.
8. White WB, Ferry JG (1992) Identification of formate dehydrogenase- specific mRNA species and nucleotide sequence of the fdhC gene of Methanobacterium formicicum. J Bacteriol 174(15):4997-5004.
9. Czyzewski BK, Wang DN (2012) Identification and characterization of a bacterial hydrosulphide ion channel. Nature 483(7390):494-497.
10. Peakman T, et al. (1990) Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome. Eur J Biochem 191(2):315-323. (Pubitemid 20233744)
11. Clegg S, Yu F, Griffiths L, Cole JA (2002) The roles of the polytopic membrane proteins NarK, NarU and NirC in Escherichia coli K-12: Two nitrate and three nitrite transporters. Mol Microbiol 44(1):143-155. (Pubitemid 34429771)
12. Wu HC, Tyson KL, Cole JA, Busby SJW (1998) Regulation of transcription initiation at the Escherichia coli nir operon promoter: A new mechanism to account for co-dependence on two transcription factors. Mol Microbiol 27(2):493-505. (Pubitemid 28035271)
13. Jia WJ, Tovell N, Clegg S, Trimmer M, Cole J (2009) A single channel for nitrate uptake, nitrite export and nitrite uptake by Escherichia coli NarU and a role for NirC in nitrite export and uptake. Biochem J 417(1):297-304.
14. Rycovska A, Hatahet L, Fendler K, Michel H (2012) The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter. Biochim Biophys Acta 1818(5):1342-1350.
15. Xie Q, Nathan C (1994) The high-output nitric oxide pathway: Role and regulation. J Leukoc Biol 56(5):576- 582.
16. Brett PJ, Burtnick MN, Su H, Nair V, Gherardini FC (2008) iNOS activity is critical for the clearance of Burkholderia mallei from infected RAW 264.7 murine macrophages. Cell Microbiol 10(2):487-498.
17. Chakravortty D, Hensel M (2003) Inducible nitric oxide synthase and control of intracellular bacterial pathogens. Microbes Infect 5(7):621-627. (Pubitemid 36645122)
18. Das P, Lahiri A, Lahiri A, Chakravortty D (2009) Novel role of the nitrite transporter NirC in Salmonella pathogenesis: SPI2-dependent suppression of inducible nitric oxide synthase in activated macrophages. Microbiology 155(Pt 8):2476- 2489.
19. Wang Y, et al. (2009) Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel. Nature 462(7272):467-472.
20. Waight AB, Love J, Wang DN (2010) Structure and mechanism of a pentameric formate channel. Nat Struct Mol Biol 17(1):31-37.
21. Lü W, et al. (2011) pH-dependent gating in a FocA formate channel. Science 332 (6027):352-354.
22. Agre P, et al. (2002) Aquaporin water channels - from atomic structure to clinical medicine. J Physiol 542(Pt 1):3-16. (Pubitemid 35190266)
23. Hilf RJC, Dutzler R (2009) A prokaryotic perspective on pentameric ligand-gated ion channel structure. Curr Opin Struct Biol 19(4):418-424.
24. Lü W, et al. (2012) The formate channel FocA exports the products of mixed-acid fermentation. Proc Natl Acad Sci USA 109(33):13254-13259.
25. Sawers RG (2005) Formate and its role in hydrogen production in Escherichia coli. Biochem Soc Trans 33(Pt 1):42-46.
26. von Heijne G, Gavel Y (1988) Topogenic signals in integral membrane proteins. Eur J Biochem 174(4):671-678.
27. Miller C (2006) ClC chloride channels viewed through a transporter lens. Nature 440 (7083):484-489.
28. Suppmann B, Sawers G (1994) Isolation and characterization of hypophosphite-resistant mutants of Escherichia coli: Identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol 11(5):965-982. (Pubitemid 24199664)
29. Wolff N, et al. (2002) Histidine pK(a) shifts and changes of tautomeric states induced by the binding of gallium-protoporphyrin IX in the hemophore HasA(SM). Protein Sci 11(4):757-765. (Pubitemid 34241285)
30. Knol J, Sjollema K, Poolman B (1998) Detergent-mediated reconstitution of membrane proteins. Biochemistry 37(46):16410-16415. (Pubitemid 28536277)
31. Kabsch W (2010) XDS. Acta Crystallogr D Biol Crystallogr 66(Pt 2):125-132.
32. Vagin A, Teplyakov A (2010) Molecular replacement with MOLREP. Acta Crystallogr D Biol Crystallogr 66(Pt 1):22-25.
33. Emsley P, Lohkamp B, Scott WG, Cowtan K (2010) Features and development of Coot. Acta Crystallogr D Biol Crystallogr 66(Pt 4):486-501.
34. Murshudov GN, Vagin AA, Dodson EJ (1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53(Pt 3):240-255. (Pubitemid 27235885)
35. Smart OS, Neduvelil JG, Wang X, Wallace BA, Sansom MS (1996) HOLE: A program for the analysis of the pore dimensions of ion channel structural models. J Mol Graph 14 (6):354- 360, 376.