Quantitative Real-Time PCR Analysis of 'Candidatus Phytoplasma mali' Without External Standard Curves;Quantitative real-time PCR Analyse von 'Candidatus Phytoplasma mali' ohne externe Standardkurven

Erwerbs-Obstbau

Volumn 54, Issue 3, 2012, Pages 147-153

  Baric, Sanja ^a  

^a Laimburg Research Centre for Agriculture and Forestry   (Italy)

Author keywords

Apple proliferation; Malus domestica; Phytoplasma; Relative quantification

Indexed keywords

CANDIDATUS PHYTOPLASMA; CANDIDATUS PHYTOPLASMA MALI; MALUS; MALUS X DOMESTICA;

EID: 84868574788     PISSN: 00140309     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10341-012-0166-7     Document Type: Article

References (25)

1. Baric S, Dalla Via J (2004) A new approach to apple proliferation detection: a highly sensitive real-time PCR assay. J Microbiol Methods 57: 135-145.
2. Baric S, Kerschbamer C, Dalla Via J (2006) TaqMan real-time PCR versus four conventional PCR assays for detection of apple proliferation phytoplasma. Plant Mol Biol Report 24: 169-184.
3. Baric S, Kerschbamer C, Vigl J, Dalla Via J (2008) Translocation of apple proliferation phytoplasma via natural root grafts-a case study. Eur J Plant Pathol 121: 207-211.
4. Baric S, Berger J, Cainelli C, Kerschbamer C, Letschka T, Dalla Via J (2011) Seasonal colonisation of apple trees by 'Candidatus Phytoplasma mali' revealed by a new quantitative TaqMan real-time PCR approach. Eur J Plant Pathol 129: 455-467.
5. Bisognin C, Schneider B, Salm H, Grando MS, Jarausch W, Moll E, Seemüller E (2008) Apple proliferation resistance in apomictic rootstocks and its relationship to phytoplasma concentration and simple sequence repeat genotypes. Phytopathology 98: 153-158.
6. Bland JM, Altman DG (1999) Measuring agreement in method comparison studies. Stat Methods Med Res 8: 135-160.
7. Costa F, Stella S, van de Weg WE, Guerra W, Cecchinel M, Dalla Via J, Koller B, Sansavini S (2005) Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh). Euphytica 141: 181-190.
8. Gachon CM, Strittmatter M, Müller DG, Kleinteich J, Küpper FC (2009) Detection of differential host susceptibility to the marine oomycete pathogen Eurychasma dicksonii by real-time PCR: not all algae are equal. Appl Environ Microbiol 75: 322-328.
9. Hogenhout SA, Oshima K, Ammar ED, Kakizawa S, Kingdom HN, Namba S (2008) Phytoplasmas: bacteria that manipulate plants and insects. Mol Plant Pathol 9: 403-423.
10. Lee IM, Davis RE, Gundersen-Rindal DE (2000) Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54: 221-255.
11. Liu ZL, Palmquist DE, Ma MG, Liu J, Alexander NJ (2009) Application of a master equation for quantitative mRNA analysis using qRT-PCR. J Biotechnol 143: 10-16.
12. Mayer CJ, Jarausch B, Jarausch W, Jelkmann W, Vilcinskas A, Gross J (2009) Cacopsylla melanoneura has no relevance as vector of apple proliferation in Germany. Phytopathology 99: 729-738.
13. Musetti R, Paolacci A, Ciuffi M, Tanzarella OA, Poliziotto R, Tubaro F, Mizzau M, Ermacora P, Badiani M, Osler R (2010) Phloem cytochemical modification and gene expression following the recovery of apple plants from apple proliferation disease. Phytopathology 100: 203-208.
14. Musetti R, Grisan S, Polizzotto R, Martini M, Padano C, Osler R (2011) Interactions between 'Candidatus Phytoplasma mali' and the apple endophyte Epicoccum nigrum in Catharanthus roseus plants. J Appl Microbiol 110: 746-756.
15. Nolan T, Hands RE, Bustin SH (2006) Quantification of mRNA using real-time RT-PCR. Nat Protoc 1: 1559-1582.
16. Pfaffl MW (2004) Quantification strategies in real-time PCR. In: Bustin SA (ed) A-Z of quantitative PCR. International University Line, La Jolla, pp 87-120.
17. Rekab D, Pirajno G, Cettul E, De Salvador FR, Firrao G (2010) On the apple proliferation symptom display and the canopy colonization pattern of "Candidatus Phytoplasma mali" in apple trees. Eur J Plant Pathol 127: 7-12.
18. Rutledge RG, Côté C (2003) Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res 31: e93.
19. Seemüller E, Kunze L, Schaper U (1984): Colonization behavior of MLO, and symptom expression of proliferation-diseased apple trees and decline-diseased pear trees over a period of several years. Z Pflanzenkrankh Pflanzenschutz 91: 525-532.
20. Seemüller E, Schneider B (2004) 'Candidatus Phytoplasma mali', 'Candidatus Phytoplasma pyri' and 'Candidatus Phytoplasma prunorum', the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectively. Int J Syst Evol Microbiol 54: 1217-1226.
21. Seemüller E, Schneider B (2007) Differences in virulence and genomic features of strains of 'Candidatus Phytoplasma mali', the apple proliferation agent. Phytopathology 97: 964-970.
22. Strauss E (2009) Phytoplasma research begins to bloom. Science 325: 388-390.
23. Torres E, Bertolini E, Cambra M, Montón C, Martín MP (2005) Real-time PCR for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16SrX) group. Mol Cell Probes 19: 334-340.
24. Velasco R, Zharkikh A, Affourtit J, Dhingra A, Cestaro A, Kalyanaraman A, Fontana P, Bhatnagar SK, Troggio M, Pruss D, Salvi S, Pindo M, Baldi P, Castelletti S, Cavaiuolo M, Coppola G, Costa F, Cova V, Dal Ri A, Goremykin V, Komjanc M, Longhi S, Magnago P, Malacarne G, Malnoy M, Micheletti D, Moretto M, Perazzolli M, Si-Ammour A, Vezzulli S, Zini E, Eldredge G, FitzGerald LM, Gutin N, Lanchbury J, Macalma T, Mitchell JT, Reid J, Wardell B, Kodira C, Chen Z, Desany B, Niazi F, Palmer L, Koepke T, Jiwan D, Schaeffer S, Krishnan V, Wu C, Chu VT, King ST, Vick J, Tao Q, Mraz A, Stormo K, Bogden R, Ederle D, Stella A, Vecchietti A, Kater MM, Masiero S, Lasserre P, Lespinasse Y, Allan AC, Bus V, Chagné D, Crowhurst RN, Gleave AP, Lavezzo E, Fawcett J, Proost S, Rouzé P, Sterck L, Toppo S, Lazzari B, Hellens RP, Durel C, Gutin A, Bumgarner RE, Gardiner SE, Skolnick M, Egholm M, van de Peer Y, Salamini F, Viola R (2010) The genome of the domesticated apple (Malus × domestica Borkh.). Nat Genet 42: 833-839.
25. Weintraub PG, Beanland L (2006) Insect vectors of phytoplasmas. Annu Rev Entomol 51: 91-111.