Evaluation of a multiplex real-time PCR assay for the detection of respiratory viruses in clinical specimens

Annals of Laboratory Medicine

Volumn 32, Issue 6, 2012, Pages 399-406

  Rheem, Insoo ^a   Park, Joowon ^a   Kim, Tae Hyun ^b   Kim, Jong Wan ^a  

^a Dankook University College of Medicine   (South Korea)

^b BK21 Plus NBM Global Research Center for Regenerative Medicine   (South Korea)

Author keywords

Limit of detection; Multiplex real time PCR; Respiratory virus

Indexed keywords

EID: 84868570466     PISSN: 22343806     EISSN: 22343814     Source Type: Journal    
DOI: 10.3343/alm.2012.32.6.399     Document Type: Article

References (37)

1. Stockton J, Ellis JS, Saville M, Clewley JP, Zambon MC. Multiplex PCR for typing and subtyping influenza and respiratory syncytial viruses. J Clin Microbiol 1998;36:2990-5. (Pubitemid 28429426)
2. Welliver RC. Review of epidemiology and clinical risk factors for severe respiratory syncytial virus (RSV) infection. J Pediatr 2003(S5);143:S112-7. (Pubitemid 37456908)
3. Ward CL, Dempsey MH, Ring CJ, Kempson RE, Zhang L, Gor D, et al. Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. J Clin Virol 2004;29:179-88. (Pubitemid 38201464)
4. McDonough EA, Barrozo CP, Russell KL, Metzgar D. A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens. Mol Cell Probes 2005;19:314-22. (Pubitemid 41278681)
5. Adcock PM, Stout GG, Hauck MA, Marshall GS. Effect of rapid viral diagnosis on the management of children hospitalized with lower respiratory tract infection. Pediatr Infect Dis J 1997;16:842-6. (Pubitemid 27402703)
6. Lam WY, Yeung AC, Tang JW, Ip M, Chan EW, Hui M, et al. Rapid multiplex nested PCR for detection of respiratory viruses. J Clin Microbiol 2007;45:3631-40. (Pubitemid 350156860)
7. Mahony J, Chong S, Merante F, Yaghoubian S, Sinha T, Lisle C, et al. Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay. J Clin Microbiol 2007;45:2965-70. (Pubitemid 47417341)
8. Casiano-Colon AE, Hulbert BB, Mayer TK, Walsh EE, Falsey AR. Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults. J Clin Virol 2003;28:169-74. (Pubitemid 37040869)
9. Falsey AR, Criddle MC, Walsh EE. Detection of respiratory syncytial virus and human metapneumovirus by reverse transcription polymerase chain reaction in adults with and without respiratory illness. J Clin Virol 2006;35:46-50. (Pubitemid 43020952)
10. Boivin G, Côte S, Déry P, De Serres G, Bergeron MG. Multiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses. J Clin Microbiol 2004;42:45-51. (Pubitemid 38082697)
11. Strålin K, Bäckman A, Holmberg H, Fredlund H, Olcén P. Design of a multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae to be used on sputum samples. APMIS 2005;113:99-111. (Pubitemid 40343579)
12. Vabret A, Mouthon F, Mourez T, Gouarin S, Petitjean J, Freymuth J. Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction. J Virol Methods 2001;97:59-66. (Pubitemid 32718741)
13. Lim G, Park TS, Suh JT, Lee HJ. Comparison of R-mix virus culture and multiplex reverse transcriptase-PCR for the rapid detection on respiratory viruses. Korean J Lab Med 2010;30:289-94.
14. Kim JS, Lim CS, Kim YK, Lee KN, Lee CK. Human bocavirus in patients with respiratory tract infection. Korean J Lab Med 2011;31:179-84.
15. Templeton KE, Scheltinga SA, Beersma MF, Kroes AC, Claas EC. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J Clin Microbiol 2004;42: 1564-9. (Pubitemid 38500797)
16. Coiras MT, Aguilar JC, García ML, Casas I, Pérez- Breña P. Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays. J Med Virol 2004;72:484-95. (Pubitemid 38133991)
17. Bellau-Pujol S, Vabret A, Legrand L, Dina J, Gouarin S, Petitjean-Lecherbonnier J, et al. Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses. J Virol Methods 2005;126: 53-63. (Pubitemid 40558743)
18. Hu A, Colella M, Tam JS, Rappaport R, Cheng SM. Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. J Clin Microbiol 2003;41:149-54. (Pubitemid 36098568)
19. FDA. Substantial equivalence determination decision summary assay only template. http://www.accessdata.fda.gov/cdrh-docs/reviews/K073029.pdf (Updated on 2007).
20. FDA. 510(k) Summary. Simplexa™ Flu A/B & RSV. http://www.access-data.fda.gov/cdrh-docs/pdf10/K102170.pdf (Updated on Nov 2010).
21. FDA. Summary of 510(k) Safety and Effectiveness. http://www.access-data. fda.gov/cdrh-docs/pdf8/K083088.pdf (Updated on May 2009).
22. FDA. 510(k) Substantial Equivalence determination decision summary. http://www.accessdata.fda.gov/cdrh-docs/pdf10/K103776.pdf (Updated on July 2011).
23. Woo PC, Chiu SS, Seto WH, Peiris M. Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients. J Clin Microbiol 1997;35:1579-81. (Pubitemid 27216837)
24. Leland DS and Emanuel D. Laboratory diagnosis of viral infections of the lung. Semin Respir Infect 1995;10:189-98. (Pubitemid 26007450)
25. Ghosh S, Champlin RE, Englund J, Giralt SA, Rolstone K, Raad I, et al. Respiratory syncytial virus upper respiratory tract illnesses in adult blood and marrow transplant recipients: combination therapy with aerosolized ribavirin and intravenous immunoglobulin. Bone Marrow Transplant 2000;25:751-5. (Pubitemid 30189516)
26. Hayden FG, Treanor JJ, Betts RF, Lobo M, Esinhart JD, Hussey EK. Safety and efficacy of the neuraminidase inhibitor GG167 in experimental human influenza. JAMA 1996;275:295-9. (Pubitemid 26028588)
27. Bruijnesteijin van Coppenraet LE, Swanink CM, van Zwet AA, Nijhuis RH, Schirm J, Wallinga JA, et al. Comparison of two commercial molecular assays for simultaneous detection of respiratory viruses in clinical samples using two automatic electrophoresis detection systems. J Virol Methods 2010;169:188-92.
28. Klaschik S, Lehmann LE, Raadts A, Hoeft A, Stuber F. Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR. Mol Biotechnol 2002; 22:231-42. (Pubitemid 35252820)
29. Gunson RN, Collins TC, Carman WF. Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J Clin Virol 2005;33: 341-4. (Pubitemid 40982527)
30. Bolotin S, Lombos E, Yeung R, Eshaghi A, Blair J, Drews SJ. Verification of the Combimatrix influenza detection assay for the detection of influenza A subtype during the 2007-2008 influenza season in Toronto, Canada. Virol J 2009;25:37.
31. Kehl SC, Henrickson KJ, Hua W, Fan J. Evaluation of the Hexaplex assay for detection of respiratory viruses in children. J Clin Microbiol 2001; 39:1696-701. (Pubitemid 32417006)
32. Pehler-Harrington K, Khanna M, Waters CR, Henrickson KJ. Rapid detection and identification of human adenovirus species by adenoplex, a multiplex PCR-enzyme hybridization assay. J Clin Microbiol 2004;42: 4072-6. (Pubitemid 39296126)
33. Lee JH, Chun JK, Kim DS, Park Y, Choi JR, Kim HS. Identification of adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus by two kinds of multiplex polymerase chain reaction (PCR) and a shell vial culture in pediatric patients with viral pneumonia. Yonsei Med J 2010;51:761-7.
34. Rand KH, Rampersaud H, Houck HJ. Comparison of two multiplex methods for detection of respiratory viruses: FilmArray RP and xTAG RVP. J Clin Microbiol 2011;49:2449-53.
35. Zaghloul MZ. Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR). Virol J 2011;8:239-43.
36. Peng Y, Xie Z, Liu J, Pang Y, Deng X, Xie Z, et al. Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay. Virol J 2011;8:337-46.
37. Kim SR, Ki CS, Lee NY. Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay. J Virol Methods 2009;156:111-6.