Methicillin-resistant Staphylococcus aureus in nasal surveillance swabs at an intensive care unit: An evaluation of the LightCycler MRSA advanced test

Annals of Laboratory Medicine

Volumn 32, Issue 6, 2012, Pages 407-412

  Huh, Hee Jin ^a   Kim, Eu Suk ^b   Chae, Seok Lae ^a  

^a Dongguk University Ilsan Hospital   (South Korea)

^b Seoul National University Bundang Hospital   (South Korea)

Author keywords

Active surveillance; Methicillin resistant Staphylococcus aureus; Real time polymerase chain reaction

Indexed keywords

EID: 84868558516     PISSN: 22343806     EISSN: 22343814     Source Type: Journal    
DOI: 10.3343/alm.2012.32.6.407     Document Type: Article

References (23)

1. Cosgrove SE, Qi Y, Kaye KS, Harbarth S, Karchmer AW, Carmeli Y. The impact of methicillin resistance in Staphylococcus aureus bacteremia on patient outcomes: mortality, length of stay, and hospital charges. Infect Control Hosp Epidemiol 2005;26:166-74. (Pubitemid 40445022)
2. Muto CA, Jernigan JA, Ostrowsky BE, Richet HM, Jarvis WR, Boyce JM, et al. SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and enterococcus. Infect Control Hosp Epidemiol 2003;24:362-86. (Pubitemid 36612323)
3. Salgado CD, Farr BM, Calfee D P. Community-acquired methicillin-resistant Staphylococcus aureus: a meta-analysis of prevalence and risk factors. Clin Infect Dis 2003;36:131-9. (Pubitemid 36143409)
4. Wertheim HF, Melles DC, Vos MC, van Leeuwen W, van Belkum A, Verbrugh HA, et al. The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis 2005;5:751-62. (Pubitemid 41688980)
5. Park BG and Lee MK. Nasal Carriage of Methicillin-Resistant Staphylococcus aureus among Healthcare Workers and Community Students in 1997 and 2006. Korean J Nosocomial Infect Control 2007;12:85-90.
6. Park SH, Jang YH, Sung H, Kim MN, Kim JS, Park YJ. Performance evaluation of BD GeneOhm MRSA PCR assay for detection of nasal colonization of methicillin-resistant Staphylococcus aureus at endemic intensive care units. Korean J Lab Med 2009;29:439-47.
7. Diederen B, van Duijn I, van Belkum A, Willemse P, van Keulen P, Kluytmans J. Performance of CHROMagar MRSA medium for detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2005;43: 1925-7.
8. Nahimana I, Francioli P, Blanc DS. Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 2006;12:1168-74. (Pubitemid 44762518)
9. Boyce JM and Havill NL. Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains. J Clin Microbiol 2008;46:350-1.
10. Reischl U, Linde HJ, Metz M, Leppmeier B, Lehn N. Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR. J Clin Microbiol 2000;38:2429-33. (Pubitemid 30414386)
11. Huletsky A, Giroux R, Rossbach V, Gagnon M, Vaillancourt M, Bernier M, et al. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol 2004;42:1875-84. (Pubitemid 38637796)
12. Safdar N, Narans L, Gordon B, Maki DG. Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods. J Clin Microbiol 2003;41:3163-6. (Pubitemid 36842316)
13. Peterson LR, Liesenfeld O, Woods CW, Allen SD, Pombo D, Patel PA, et al. Multicenter evaluation of the LightCycler methicillin-resistant Staphylococcus aureus (MRSA) advanced test as a rapid method for detection of MRSA in nasal surveillance swabs. J Clin Microbiol 2010;48:1661-6.
14. Paule SM, Hacek DM, Kufner B, Truchon K, Thomson RB Jr, Kaul KL, et al. Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J Clin Microbiol 2007;45:2993-8. (Pubitemid 47417345)
15. Bischof LJ, Lapsley L, Fontecchio K, Jacosalem D, Young C, Hankerd R, et al. Comparison of chromogenic media to BD GeneOhm methicillinresistant Staphylococcus aureus (MRSA) PCR for detection of MRSA in nasal swabs. J Clin Microbiol 2009;47:2281-3.
16. Kim OS, Yoon SW, Kang YJ, Kim YK, Lee NY, Lee JH, et al. Rate of nasal colonization of Methicillin-Resistant Staphylococcus aureus at admission to a medical intensive care unit. Korean J Nosocomial Infect Control 2007;12:42-9.
17. de San N, Denis O, Gasasira MF, De Mendonca R, Nonhoff C, Struelens MJ. Controlled evaluation of the IDI-MRSA assay for detection of colonization by methicillin-resistant Staphylococcus aureus in diverse mucocutaneous specimens. J Clin Microbiol 2007;45:1098-101. (Pubitemid 46626470)
18. Rossney AS, Herra CM, Fitzgibbon MM, Morgan PM, Lawrence MJ, O'Connell B. Evaluation of the IDI-MRSA assay on the SmartCycler realtime PCR platform for rapid detection of MRSA from screening specimens. Eur J Clin Microbiol Infect Dis 2007;26:459-66. (Pubitemid 47025030)
19. Martineau F, Picard FJ, Lansac N, Ménard C, Roy PH, Ouellette M, et al. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob Agents Chemother 2000;44:231-8. (Pubitemid 30060711)
20. Donnio PY, Oliveira DC, Faria NA, Wilhelm N, Le Coustumier A, de Lencastre H. Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus. J Clin Microbiol 2005;43:4191-3. (Pubitemid 41129723)
21. Farley JE, Stamper PD, Ross T, Cai M, Speser S, Carroll KC. Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population. J Clin Microbiol 2008;46:743-6. (Pubitemid 351231327)
22. Donnio PY, Fevrier F, Bifani P, Dehem M, Kervegant C, Wilhelm N, et al. Molecular and epidemiological evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals. Antimicrob Agents Chemother 2007;51:4342-50. (Pubitemid 350209887)
23. Drews SJ, Willey BM, Kreiswirth N, Wang M, Ianes T, Mitchell J, et al. Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting. J Clin Microbiol 2006;44:3794-6. (Pubitemid 46768790)