Performance evaluation of BD geneohm MRSA PCR assay for detection of nasal colonization of methicillin-resistant Staphylococcus aureus at endemic intensive care units

Korean Journal of Laboratory Medicine

Volumn 29, Issue 5, 2009, Pages 439-447

  Park, Sang Hyuk ^a   Jang, Yoon Ha ^a   Sung, Heungsup ^a   Kim, Mi Na ^a   Kim, Jae Suk ^b   Park, Yeon Joon ^c  

^a University of Ulsan College of Medicine   (South Korea)

^b Hallym University College of Medicine   (South Korea)

^c The Catholic University of Korea   (South Korea)

Author keywords

BD GeneOhm; MRSA; Nasal colonization; Performance

Indexed keywords

ANALYTICAL EQUIPMENT; ARTICLE; ENDEMIC DISEASE; EVALUATION; GENETICS; HUMAN; INTENSIVE CARE UNIT; ISOLATION AND PURIFICATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHODOLOGY; MICROBIOLOGY; NOSE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCUS INFECTION;

ENDEMIC DISEASES; HUMANS; INTENSIVE CARE UNITS; METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS; NOSE; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY; STAPHYLOCOCCAL INFECTIONS;

EID: 77949311881     PISSN: 15986535     EISSN: None     Source Type: Journal    
DOI: 10.3343/kjlm.2009.29.5.439     Document Type: Article

References (39)

1. Chambers HF. The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 2001;7:178-82.
2. Chong YS and Lee K. Present situation of antimicrobial resistance in Korea. J Infect Chemother 2000;6:189-95.
3. Lee K, Park KH, Jeong SH, Lim HS, Shin JH, Yong D, et al. Further increase of vancomycin-resistant Enterococcus faecium, amikacin- and fluoroquinolone-resistant Klebsiella pneumoniae, and imipenem-resistant Acinetobacter spp. in Korea: 2003 KONSAR surveillance. Yonsei Med J 2006;47:43-54.
4. Kim KM, Yoo JH, Choi JH, Park ES, Kim KS, Kim KS, et al. The nationwide surveillance results of nosocomial infections along with antimicrobial resistance in intensive care units of sixteen university hospitals in Korea, 2004. Korean J Nosocomial Infect Control 2006;11:79-86. (Korean Source 2004Korean Source 16Korean Source 2006;11:79-86.).
5. Wertheim HF, Vos MC, Boelens HA, Voss A, Vandenbroucke-Grauls CM, Meester MH, et al. Low prevalence of methicillin-resistant Staphylococcus aureus (MRSA) at hospital admission in the Netherlands: the value of search and destroy and restrictive antibiotic use. J Hosp Infect 2004;56:321-5.
6. Siegel JD, Rhinehart E, Jackson M, Chiarello L. Management of multidrug-resistant organisms in health care settings, 2006. Am J Infect Control 2007;35(S):S165-93.
7. Smith PW, Bennett G, Bradley S, Drinka P, Lautenbach E, Marx J, et al. SHEA/APIC Guideline: Infection prevention and control in the long-term care facility. Am J Infect Control 2008;36:504-35.
8. Weber SG, Huang SS, Oriola S, Huskins WC, Noskin GA, Harriman K, et al. Legislative mandates for use of active surveillance cultures to screen for methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci: position statement from the Joint SHEA and APIC Task Force. Infect Control Hosp Epidemiol 2007;28:249-60.
9. Sewell DL, Potter SA, Jacobson CM, Strausbaugh LJ, Ward TT. Sensitivity of surveillance cultures for the detection of methicillin-resistant Staphylococcus aureus in a nursing-home-care unit. Diagn Microbiol Infect Dis 1993;17:53-6.
10. Nonhoff C, Denis O, Brenner A, Buidin P, Legros N, Thiroux C, et al. Comparison of three chromogenic media and enrichment broth media for the detection of methicillin-resistant Staphylococcus aureus from mucocutaneous screening specimens: Comparison of MRSA chromogenic media. Eur J Clin Microbiol Infect Dis 2009;28:363-9.
11. Nahimana I, Francioli P, Blanc DS. Evaluation of three chromogenic media (MRSA-ID, MRSA-Select, CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 2006;12:1168-74.
12. Murray PR, Baron EJ, et al. eds. Manuals of clinical mircobiology. 9th ed. Washington DC: American society of microbiology press, 2007:122-3.
13. Jaffe RI, Lane JD, Albury SV, Niemeyer DM. Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR. J Clin Microbiol 2000;38:3407-12.
14. Pai CH, Kim MN, et al. eds. Clinical Microbiology for Infection Con-trol. 1st ed. Seoul: Hanmi medical publishing, 2007:90-1. (Korean Source 1st. ed. Korean Source 2007; 90-1.).
15. Cuny C and Witte W. PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosomeborne orfX. Clin Microbiol Infect 2005;11:834-7.
16. Paule SM, Hacek DM, Kufner B, Truchon K, Thomson RB Jr, Kaul KL, et al. Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J Clin Microbiol 2007;45:2993-8.
17. Huletsky A, Giroux R, Rossbach V, Gagnon M, Vaillancourt M, Bernier M, et al. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol 2004;42:1875-84.
18. Hiramatsu K, Cui L, Kuroda M, Ito T. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends Microbiol 2001; 9:486-93.
19. Farley JE, Stamper PD, Ross T, Cai M, Speser S, Carroll KC. Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population. J Clin Microbiol 2008;46:743-6.
20. BD GeneOhm™ MRSA PCR assay package insert. http://www.bd.com/geneohm/english/products/idi_mrsa.asp(Cited May 2008).
21. Rossney AS, Herra CM, Fitzgibbon MM, Morgan PM, Lawrence MJ, O'Connell B. Evaluation of the IDI-MRSA assay on the SmartCycler real-time PCR platform for rapid detection of MRSA from screening specimens. Eur J Clin Microbiol Infect Dis 2007;26:459-66.
22. Bartels MD, Boye K, Rohde SM, Larsen AR, Torfs H, Bouchy P, et al. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. J Clin Microbiol 2009;47:1524-7.
23. Chung HJ, Jeon HS, Sung H, Kim MN, Hong SJ. Epidemiological characteristics of methicillin-resistant Staphylococcus aureus isolates from children with eczematous atopic dermatitis lesions. J Clin Microbiol 2008;46:991-5.
24. Ko KS, Lee JY, Suh JY, Oh WS, Peck KR, Lee NY, et al. Distribution of major genotypes among methicillin-resistant Staphylococcus aureus clones in Asian countries. J Clin Microbiol 2005;43:421-6.
25. Chongtrakool P, Ito T, Ma XX, Kondo Y, Trakulsomboon S, Tiensasitorn C, et al. Staphylococcal cassette chromosome mec (SCCmec) typing of methicillin-resistant Staphylococcus aureus strains isolated in 11 Asian countries: a proposal for a new nomenclature for SCCmec elements. Antimicrob Agents Chemother 2006;50:1001-12.
26. Kim JS, Song W, Kim HS, Cho HC, Lee KM, Choi MS, et al. Association between the methicillin resistance of clinical isolates of Staphylococcus aureus, their staphylococcal cassette chromosome mec (SCCmec) subtype classification, and their toxin gene profiles. Diagn Microbiol Infect Dis 2006;56:289-95.
27. Nsira SB, Dupuis M, Leclercq R. Evaluation of MRSASelect, a new chromogenic medium for the detection of nasal carriage of methicillin-resistant Staphylococcus aureus. Int J Antimicrob Agents 2006; 27:561-4.
28. Park C, Lee DG, Kim SW, Choi SM, Park SH, Chun HS, et al. Predominance of community-associated methicillin-resistant Staphylococcus aureus strains carrying staphylococcal chromosome cassette mec type IVA in South Korea. J Clin Microbiol 2007;45:4021-6.
29. Louie L, Soares D, Meaney H, Vearncombe M, Simor AE. Evaluation of a new chromogenic medium, MRSA select, for detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 2006;44:4561-3.
30. Krishna BV, Smith M, McIndeor A, Gibb AP, Dave J. Evaluation of chromogenic MRSA medium, MRSA select and Oxacillin Resistance Screening Agar for the detection of methicillin-resistant Staphylococcus aureus. J Clin Pathol 2008;61:841-3.
31. An DH, Jeon HS, Park SJ, Sung H, Kim MN. Evaluation of MRSASelect for detecting methicillin-resistant Staphylococcus aureus from surveillance specimens. Korean J Nosocomial Infect Control 2007;12:28-35. (Korean Source Methicillin Korean Source MRSASelect Korean Source 2007;12:28-35.).
32. Daskalaki M, Otero JR, Sanz F, Chaves F. Bacteremia due to clonally derived methicillin-resistant, gentamicin-susceptible isolates and methicillin-susceptible, gentamicin-resistant isolates of Staphylococcus aureus. J Clin Microbiol 2007;45:3446-8.
33. Donnio PY, Fevrier F, Bifani P, Dehem M, Kervegant C, Wilhelm N, et al. Molecular and epidemiological evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals. Antimicrob Agents Chemother 2007;51:4342-50.
34. Francois P, Bento M, Renzi G, Harbarth S, Pittet D, Schrenzel J. Evaluation of three molecular assays for rapid identification of methicillin resistant Staphylococcus aureus. J Clin Microbiol 2007;45:2011-3.
35. Brown DF, Edwards DI, Hawkey PM, Morrison D, Ridgway GL, Towner KJ, et al. Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 2005;56:1000-18.
36. Harbarth S, Masuet-Aumatell C, Schrenzel J, Francois P, Akakpo C, Renzi G, et al. Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care: an interventional cohort study. Crit Care 2006;10:R25.
37. Harbarth S, Fankhauser C, Schrenzel J, Christenson J, Gervaz P, Bandiera-Clerc C, et al. Universal screening for methicillin-resistant Staphylococcus aureus at hospital admission and nosocomial infection in surgical patients. JAMA 2008;299:1149-57.
38. Pearman JW. 2004 Lowbury Lecture: the Western Australian experience with vancomycin-resistant enterococci-from disaster to ongoing control. J Hosp Infect 2006;63:14-26.
39. Christiansen KJ, Tibbett PA, Beresford W, Pearman JW, Lee RC, Coombs GW, et al. Eradication of a large outbreak of a single strain of vanB vancomycin-resistant Enterococcus faecium at a major Australian teaching hospital. Infect Control Hosp Epidemiol 2004;25: 384-90.