Limitations of an ocular surface inflammatory biomarker in impression cytology specimens

Cutaneous and Ocular Toxicology

Volumn 32, Issue 1, 2013, Pages 46-53

  Yafawi, Rolla ^a   Ko, Mira ^a   Sace, Frederick P ^a   John Baptiste, Annette ^a  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

Author keywords

Flow cytometry; HLA DR; Impression cytology

Indexed keywords

HLA DR1 ANTIGEN; INTERCELLULAR ADHESION MOLECULE 1; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2;

ACCURACY; ADULT; ARTICLE; CELL POPULATION; CELL SIZE; CELLULAR DISTRIBUTION; CLINICAL ARTICLE; CONJUNCTIVA EPITHELIUM; CONTROLLED STUDY; DISEASE SEVERITY; DRUG DEVELOPMENT; DRY EYE; EYE INFLAMMATION; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMPRESSION CYTOLOGY; NORMAL HUMAN; PROTEIN EXPRESSION; REPRODUCIBILITY;

ADULT; BIOLOGICAL MARKERS; CONJUNCTIVA; DRY EYE SYNDROMES; EPITHELIAL CELLS; FLOW CYTOMETRY; HLA-DR ANTIGENS; HUMANS; INFLAMMATION;

EID: 84868520838     PISSN: 15569527     EISSN: 15569535     Source Type: Journal    
DOI: 10.3109/15569527.2012.705405     Document Type: Article

References (19)

1. Scott CA, Catania LJ, Larkin KM, Melton R, Semes LP, Shovlin JP. Care of the patient with ocular disorders. 2011. Available at: http://www. aoa.org/documents/CPG-10.pdf. Accessed on: 29 August 2011
2. Miljanovic B, Dana R, Sullivan DA, Schaumberg DA. Impact of dry eye syndrome on vision-related quality of life. Am J Ophthalmol 2007;143:409-415
3. Kamao T, Yamaguchi M, Kawasaki S, Mizoue S, Shiraishi A, Ohashi Y. Screening for dry eye with newly developed ocular surface thermographer. Am J Ophthalmol 2011;151:782-791.e1
4. Brignole F, Pisella PJ, Goldschild M, De Saint Jean M, Goguel A, Baudouin C. Flow cytometric analysis of inflammatory markers in conjunctival epithelial cells of patients with dry eyes. Invest Ophthalmol Vis Sci 2000;41:1356-1363
5. Stern ME, Gao J, Schwalb TA, Ngo M, Tieu DD, Chan CC et al. Conjunctival T-cell subpopulations in Sjogren's and non-Sjogren's patients with dry eye. Invest Ophthalmol Vis Sci 2002;43:2609-2614
6. Pflugfelder SC. Anti-inflammatory therapy for dry eye. Am J Ophthalmol 2004;137:337-342
7. Mrugacz M, Zywalewska N. HLA-DR antigen expression on conjunctival epithelial cells in patients with dry eye. Klin Oczna 2005;107:278-279
8. Baudouin C, Brignole F, Becquet F, Pisella PJ, Goguel A. Flow cytometry in impression cytology specimens. A new method for evaluation of conjunctival inflammation. Invest Ophthalmol Vis Sci 1997;38:1458-1464
9. Brignole-Baudouin F, Ott AC, Warnet JM, Baudouin C. Flow cytometry in conjunctival impression cytology: A new tool for exploring ocular surface pathologies. Exp Eye Res 2004;78:473-481
10. Brignole F, Pisella PJ, De Saint Jean M, Goldschild M, Goguel A, Baudouin C. Flow cytometric analysis of inflammatory markers in KCS: 6-month treatment with topical cyclosporin A. Invest Ophthalmol Vis Sci 2001;42:90-95
11. Thatcher RW, Darougar S, Jones BR. Conjunctival impression cytology. Arch Ophthalmol 1977;95:678-681
12. Lopin E, Deveney T, Asbell PA. Impression cytology: Recent advances and applications in dry eye disease. Ocul Surf 2009;7:93-110
13. Tsubota K, Fujihara T, Saito K, Takeuchi T. Conjunctival epithelium expression of HLA-DR in dry eye patients. Ophthalmologica 1999;213:16-19
14. Mrugacz M, Zak J, Bakunowicz-Lazarczyk A, Wysocka J, Minarowska A. Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis. Eye (Lond) 2007;21:1062-1066
15. Hingorani M, Calder VL, Buckley RJ, Lightman SL. The role of conjunctival epithelial cells in chronic ocular allergic disease. Exp Eye Res 1998;67:491-500
16. Pisella PJ, Malet F, Lejeune S, Brignole F, Debbasch C, Bara J et al. Ocular surface changes induced by contact lens wear. Cornea 2001;20:820-825
17. Lee JW, Devanarayan V, Barrett YC, Weiner R, Allinson J, Fountain S et al. Fit-for-purpose method development and validation for successful biomarker measurement. Pharm Res 2006;23:312-328
18. Davis C, Wu X, Li W, Fan H, Reddy M. Stability of immunophenotypic markers in fixed peripheral blood for extended analysis using flow cytometry. J Immunol Methods 2011;363:158-165
19. Wu DY, Patti-Diaz L, Hill CG. Development and validation of flow cytometry methods for pharmacodynamic clinical biomarkers. Bioanalysis 2010;2:1617-1626