Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis

Eye

Volumn 21, Issue 8, 2007, Pages 1062-1066

  Mrugacz, M ^a   Zak, J ^a   Bakunowicz Lazarczyk, A ^a   Wysocka, J ^a   Minarowska, A ^a  

^a MEDICAL UNIVERSITY OF BIALYSTOK   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

AMBROXOL; ANTIINFLAMMATORY AGENT; COLISTIMETHATE; DYE; HLA DR ANTIGEN; MONOCLONAL ANTIBODY; PANCREAS ENZYME; RETINOL;

ADOLESCENT; ADULT; ANTIGEN EXPRESSION; ARTICLE; CLINICAL ARTICLE; CONJUNCTIVA EPITHELIUM; CONTROLLED STUDY; CYSTIC FIBROSIS; EPITHELIUM CELL; EYE EXAMINATION; FEMALE; FLOW CYTOMETRY; GRAM NEGATIVE INFECTION; HUMAN; IMMUNOGENICITY; IMPRESSION CYTOLOGY; INFLAMMATION; INTERMETHOD COMPARISON; KERATOCONJUNCTIVITIS SICCA; MALE; PATHOGENESIS; PSEUDOMONAS AERUGINOSA; SCHIRMER TEST; SCHOOL CHILD; STAINING; TEAR FILM;

EID: 34547930922     PISSN: 0950222X     EISSN: 14765454     Source Type: Journal    
DOI: 10.1038/sj.eye.6702435     Document Type: Article

References (33)

1. Riodan JR, Rommens JM, Kerem BS. Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA. Science 1989; 245: 1066-1073.
2. Mc Kone EF, Emerson SS, Edwards KL, Aitken ML. Effect of genotype on phenotype and mortality in cystic fibrosis: A retrospective cohort study. Lancet 2003; 361: 1671-1676.
3. Nagel G. Differential function of the two nucleotide binding domains on cystic fibrosis transmembrane conductance regulator. Biochim Biophys Acta 1999; 1461: 263-274.
4. We lsh MJ, Anderson MP, Rich DP. Cystic fibrosis, CFTR and abnormal electrolyte transport. In: Davis PB (eds). Lung Biology in Health and Disease. Marcel Dekker: New York, 1993 pp 29-32.
5. Kerem BS, Rommens JM, Buchanan JA. Identification of the cystic fibrosis gene: Genetic analysis. Science 1989; 245: 1073-1080.
6. Mickle JE, Cutting GR. Genotype-phenotype relationships in cystic fibrosis. Med Clin N Am 2000; 84: 597-607.
7. Castagna I, Roszkowska AM, Fama F, Sinicropi S, Ferreri G. The eye in cystic fibrosis. Eur J Ophthalmol 2001; 11: 9-14.
8. Turner HC, Bernstein A, Candia OA. Presence of CFTR in the conjunctival epithelium. Curr Eye Res 2002; 24: 182-187.
9. Baudouin C. The pathology of dry eye. Surv Ophthalmol 2001; 45 211-220.
10. Brignole F, Pisella PJ, Goldshild M, De Saint Jean M, Goguel A, Baudouin C. Flow cytometric analysis of inflammatory markers in conjunctival epithelial cells of patients with dry eyes. Invest Ophthalmol Vis Sci 2000; 41: 1356-1363.
11. Brignole F, Pisella PJ, Goldshild M, De Saint Brignole F, Pisella PJ, De Saint Jean M et al. Flow cytometric analysis of inflammatory markers in KCS: 6-month treatment with topical cyclosporin A. Invest Ophthalmol Vis Sci 2001; 42: 90-95.
12. Gao J, Morgan G, Tieu D, Schwalb TA, Luo JY, Wheeler LA et al. ICAM-1 expression predisposes ocular tissue to immune-based inflammation in dry eye patients and Sjogrens syndrome-like MRL/1pr mice. Exp Eye Res 2004; 78:823-835.
13. Pflugfelder SC. Antiinflammatory therapy for dry eye. Am J Ophthalmol 2004; 137: 337-342.
14. Pisella PJ, Brignole F, Debbasch C, Pharm D, Lozato PA, Creuzot-Garcher C et al. Flow cytometric analysis of conjunctival epithelium in ocular rosacea and keratoconjunctivitis sicca. Ophthalmology 2000; 107: 1841-1849.
15. Stern ME, Gao J, Siemasko KF, Beuerman RW, Pflugfelder SC. The role of the lacrimal functional unit in the pathophysiology of dry eye. Exp Eye Res 2004; 78: 409-416.
16. Bron AJ, Mengher IS, Davey CC. The normal conjunctiva and its responses to inflammation. Trans Ophthalmol 1985; 104: 424-434.
17. Records RE. The conjunctiva and lacrimal system. In: Duane TD, Jaeger EA (eds). Biomedical Foundations of Ophthalmology. JB Lippincott Co.: Philadelphia, USA, 1998 pp 1-21.
18. Tsubota K, Fujihara T, Saito K, Takeuchi T. Conjunctival epithelium expression of HLA-DR in dry eye patients. Ophthalmologica 1999; 213: 16-19.
19. Chandler JW, Gillette TE. Immunologic defense mechanisms of the ocular surface. Ophthalmology 1983; 90: 585-591.
20. Baudouin C, Brignole F, Becquet F, Pisella PJ, Goguel A. Flow cytometry in impression cytology specimens. A new method for evaluation of conjunctival inflammation. Invest Ophthalmol Vis Sci 1997, 38 1458-1464.
21. Stern ME, Gao J, Schwalb TA, Ngo M, Tieu DD, Chain CC et al. Conjunctival T-cell subpopulations in Sjogren's and non-Sjogren's patients with dry eye. Invest Ophthalmol Vis Sci 2002; 43: 2609-2614.
22. Zhan H, Towler HM, Calder VL. The immunomodulatory role of human conjunctival epithelial cells. Invest Ophthalmol Vis Sci 2003; 44: 3906-3910.
23. Calonge M, Diebold Y, Saez V, de Salamanca AE, Garcia-Vazquez C, Corrales RM et al. Impression cytology of the ocular surface: A review. Exp Eye Res 2000, 78: 457-472.
24. Morkeberg JC, Edmund C, Prause JU, Lanng S, Koch C, Michaelsen KF. Ocular findings in cystic fibrosis patients receiving vitamin A supplementation. Exp Eye Res 1995; 233: 709-713.
25. Shepard JD, Orenstein DM, Chao ChCh, Butala S, Kowalski RP. The ocular surface in cystic fibrosis. Ophthalmology 1989, 96: 1624-1630.
26. Brignole-Baudouin F, Ott AC, Warnet JM, Baudouin C. Flow cytometry in conjunctival impression cytology: A new tool for exploring ocular surface pathologies. Exp Eye Res 2004, 78: 473-481.
27. Jones D, Monroy D, Ji Z, Atherton SS, Pflugfelder SC. Sjogren's syndrome: Cytokine and Epstein-Barr viral gene expression within the conjunctival epithelium. Invest Ophthalmol Via Sci 1994; 35: 3493-3504.
28. Tsubota K, Fukagawa K, Fujihara T, Shimmura S, Saito I, Saito K et al. Regulation of human leukocyte antigen expression in human conjunctival epithelium. Invest Ophthalmol Vis Sci 1999; 40: 28-34.
29. Pisella PJ, Brignole F, Lejeune S, Brignole F, Debbasch C, Bara J et al. Ocular surface changes induced by contact lens wear. Cornea 2001; 20: 820-825.
30. Barton K, Monroy DC, Nava A, Pflugfelder SC. Inflammatory cytokines in the tears of patients with ocular rosacea. Ophthalmology 1997; 104: 1868-1874.
31. Afonso AA, Sobrin L, Monroy DC. Tear fluid gelatinase B activity correlates with IL-1 alpha concentration and fluorescein clearance in ocular rosacea. Invest Ophthalmol Vis Sci 1999; 40: 2506-2512.
32. Hingorani M, Calder V, Buckley RJ, Lightman SL. The role of conjunctival epithelial cells in chronic ocular allergic disease. Exp Eye Res 1998; 67: 491-500.
33. Clayton A, Court J, Navabi H, Adams M, Mason MD, Hobot JA et al. Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry. J Immunol Meth 2001; 247: 163-174.