Immobilized Metal Affinity Chromatography Co-Purifies TGF-β1 with Histidine-Tagged Recombinant Extracellular Proteins

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Kaur, Jasvir ^a   Reinhardt, Dieter P ^a,b  

^a MCGILL UNIVERSITY   (Canada)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BONE MORPHOGENETIC PROTEIN 2; MESSENGER RNA; NICKEL; RECOMBINANT FIBRILLIN 1; RECOMBINANT PROTEIN; SMAD2 PROTEIN; SMAD3 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIBODY AFFINITY; ARTICLE; CHILD; CONTROLLED STUDY; EMBRYO; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INTRACELLULAR SIGNALING; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PRESCHOOL CHILD; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; PROTEIN SECRETION; WESTERN BLOTTING;

BLOTTING, WESTERN; CELLS, CULTURED; CHILD, PRESCHOOL; CHROMATOGRAPHY, AFFINITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; EXTRACELLULAR MATRIX PROTEINS; HEK293 CELLS; HISTIDINE; HUMANS; IMMOBILIZED PROTEINS; MALE; METALS; MICROFILAMENT PROTEINS; NICKEL; PEPTIDE FRAGMENTS; PHOSPHORYLATION; PROTEIN BINDING; RECOMBINANT PROTEINS; SMAD2 PROTEIN; SMAD3 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84868320653     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0048629     Document Type: Article

References (43)

1. Reinhardt DP, Keene DR, Corson GM, Pöschl E, Bächinger HP, et al. (1996) Fibrillin 1: organization in microfibrils and structural properties. J Mol Biol 258: 104-116.
2. Kortesmaa J, Yurchenco P, Tryggvason K, (2000) Recombinant laminin-8 (alpha(4)beta(1)gamma(1)). Production, purification, and interactions with integrins. J Biol Chem 275: 14853-14859.
3. Lomas AC, Mellody KT, Freeman LJ, Bax DV, Shuttleworth CA, et al. (2007) Fibulin-5 binds human smooth-muscle cells through alpha5beta1 and alpha4beta1 integrins, but does not support receptor activation. Biochem J 405: 417-428.
4. Hirani R, Hanssen E, Gibson MA, (2007) LTBP-2 specifically interacts with the amino-terminal region of fibrillin-1 and competes with LTBP-1 for binding to this microfibrillar protein. Matrix Biol 26: 213-223.
5. Bax DV, Bernard SE, Lomas A, Morgan A, Humphries J, et al. (2003) Cell adhesion to fibrillin-1 molecules and microfibrils is mediated by alpha5 beta1 and alphav beta3 integrins. J Biol Chem 278: 34605-34616.
6. Ruggiero F, Koch M, (2008) Making recombinant extracellular matrix proteins. Methods 45: 75-85.
7. Porath J, Carlsson J, Olsson I, Belfrage G, (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258: 598-599.
8. Hochuli E, Dobeli H, Schacher A, (1987) New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr 411: 177-184.
9. Janknecht R, De MG, Lou J, Hipskind RA, Nordheim A, et al. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci USA 88: 8972-8976.
10. Zhang Y, Cao L, Kiani CG, Yang BL, Yang BB, (1998) The G3 domain of versican inhibits mesenchymal chondrogenesis via the epidermal growth factor-like motifs. J Biol Chem 273: 33054-33063.
11. Saito M, Kurokawa M, Oda M, Oshima M, Tsutsui K, et al. (2011) ADAMTSL6beta protein rescues fibrillin-1 microfibril disorder in a Marfan syndrome mouse model through the promotion of fibrillin-1 assembly. J Biol Chem 286: 38602-38613.
12. Feng JQ, Guo FJ, Jiang BC, Zhang Y, Frenkel S, et al. (2010) Granulin epithelin precursor: a bone morphogenic protein 2-inducible growth factor that activates Erk1/2 signaling and JunB transcription factor in chondrogenesis. FASEB J 24: 1879-1892.
13. Gao X, Joselin AP, Wang L, Kar A, Ray P, et al. (2010) Progranulin promotes neurite outgrowth and neuronal differentiation by regulating GSK-3beta. Protein Cell 1: 552-562.
14. Koch M, Murrell JR, Hunter DD, Olson PF, Jin W, et al. (2000) A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization. J Cell Biol 151: 221-234.
15. Low FN, (1962) Microfibrils: fine filamentous components of the tissue space. Anat Rec 142: 131-137.
16. Ross R, Bornstein P, (1969) The elastic fiber: the separation and partial characterization of its macromolecular components. J Cell Biol 40: 366-381.
17. Sakai LY, Keene DR, Engvall E, (1986) Fibrillin, a new 350-kD glycoprotein, is a component of extracellular microfibrils. J Cell Biol 103: 2499-2509.
18. Thomas P, Smart TG, (2005) HEK293 cell line: a vehicle for the expression of recombinant proteins. J Pharmacol Toxicol Methods 51: 187-200.
19. Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, et al. (2008) Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J Biol Chem 283: 13874-13888.
20. Sengle G, Ono RN, Sasaki T, Sakai LY, (2011) Prodomains of transforming growth factor beta (TGFbeta) superfamily members specify different functions: extracellular matrix interactions and growth factor bioavailability. J Biol Chem 286: 5087-5099.
21. Gentry LE, Webb NR, Lim GJ, Brunner AM, Ranchalis JE, et al. (1987) Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells. Mol Cell Biol 7: 3418-3427.
22. Gentry LE, Lioubin MN, Purchio AF, Marquardt H, (1988) Molecular events in the processing of recombinant type 1 pre-pro-transforming growth factor beta to the mature polypeptide. Mol Cell Biol 8: 4162-4168.
23. Taipale J, Miyazono K, Heldin CH, Keski-Oja J, (1994) Latent transforming growth factor-ß1 associates to fibroblast extracellular matrix via latent TGF-ß binding protein. J Cell Biol 124: 171-181.
24. Miyazono K, Olofsson A, Colosetti P, Heldin CH, (1991) A role of the latent TGF-ß1-binding protein in the assembly and secretion of TGF-ß1. EMBO J 10: 1091-1101.
25. Annes JP, Munger JS, Rifkin DB, (2003) Making sense of latent TGFbeta activation. J Cell Sci 116: 217-224.
26. Laiho M, Weis MB, Massague J, (1990) Concomitant loss of transforming growth factor (TGF)-beta receptor types I and II in TGF-beta-resistant cell mutants implicates both receptor types in signal transduction. J Biol Chem 265: 18518-18524.
27. Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, et al. (1992) TGF beta signals through a heteromeric protein kinase receptor complex. Cell 71: 1003-1014.
28. Wrana JL, Attisano L, Wieser R, Ventura F, Massague J, (1994) Mechanism of activation of the TGF-beta receptor. Nature 370: 341-347.
29. Zhang Y, Feng X, We R, Derynck R, (1996) Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Nature 383: 168-172.
30. Macias-Silva M, Abdollah S, Hoodless PA, Pirone R, Attisano L, et al. (1996) MADR2 is a substrate of the TGFbeta receptor and its phosphorylation is required for nuclear accumulation and signaling. Cell 87: 1215-1224.
31. Massague J, (1998) TGF-beta signal transduction. Annu Rev Biochem 67: 753-791.
32. Morgan TL, Yang DJ, Fry DG, Hurlin PJ, Kohler SK, et al. (1991) Characteristics of an infinite life span diploid human fibroblast cell strain and a near-diploid strain arising from a clone of cells expressing a transfected v-myc oncogene. Exp Cell Res 197: 125-136.
33. Kettle S, Card CM, Hutchinson S, Sykes B, Handford PA, (2000) Characterisation of fibrillin-1 cDNA clones in a human fibroblast cell line that assembles microfibrils. Int J Biochem Cell Biol 32: 201-214.
34. Jensen SA, Reinhardt DP, Gibson MA, Weiss AS, (2001) MAGP-1, Protein interaction studies with tropoelastin and fibrillin-1. J Biol Chem 276: 39661-39666.
35. Reinhardt DP, Sasaki T, Dzamba BJ, Keene DR, Chu ML, et al. (1996) Fibrillin-1 and fibulin-2 interact and are colocalized in some tissues. J Biol Chem 271: 19489-19496.
36. Simpson RJ, (2007) Staining proteins in gels with silver nitrate. Cold Spring Harb Protoc 2007: pdb.prot4727.
37. Lin G, Tiedemann K, Vollbrandt T, Peters H, Bätge B, et al. (2002) Homo- and heterotypic fibrillin-1 and-2 interactions constitute the basis for the assembly of microfibrils. J Biol Chem 277: 50795-50804.
38. Rock MJ, Cain SA, Freeman LJ, Morgan A, Mellody K, et al. (2004) Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link. J Biol Chem 279: 23748-23758.
39. Ono RN, Sengle G, Charbonneau NL, Carlberg V, Bachinger HP, et al. (2009) Latent transforming growth factor beta-binding proteins and fibulins compete for fibrillin-1 and exhibit exquisite specificities in binding sites. J Biol Chem 284: 16872-16881.
40. Isogai Z, Ono RN, Ushiro S, Keene DR, Chen Y, et al. (2003) Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated protein. J Biol Chem 278: 2750-2757.
41. Weinbaum JS, Broekelmann TJ, Pierce RA, Werneck CC, Segade F, et al. (2008) Deficiency in microfibril-associated glycoprotein-1 leads to complex phenotypes in multiple organ systems. J Biol Chem 283: 25533-25543.
42. Wakefield LM, Smith DM, Broz S, Jackson M, Levinson AD, et al. (1989) Recombinant TGF-beta 1 is synthesized as a two-component latent complex that shares some structural features with the native platelet latent TGF-beta 1 complex. Growth Factors 1: 203-218.
43. Shi M, Zhu J, Wang R, Chen X, Mi L, et al. (2011) Latent TGF-beta structure and activation. Nature 474: 343-349.