A fluorescent screening platform for the rapid evaluation of chemicals in cellular reprogramming

Stem Cell Research

Volumn 9, Issue 3, 2012, Pages 185-191

  Vendrell, Marc ^a,d   Park, Sung Jin ^a   Chandran, Yogeswari ^a   Lee, Chi Lik Ken ^b   Ha, Hyung Ho ^c,e   Kang, Nam Young ^a   Yun, Seong Wook ^a   Chang, Young Tae ^a,c  

^a AGENCY FOR SCIENCE   (Singapore)

^b SINGAPORE POLYTECHNIC   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d UNIVERSITY OF EDINBURGH   (United Kingdom)

^e Sunchon National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; HISTONE DEACETYLASE INHIBITOR; HYDROXAMIC ACID DERIVATIVE; KLF 4 PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN; TRANSCRIPTION FACTOR SOX2; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MATURATION; CHEMICAL ANALYSIS; FIBROBLAST; FLUORESCENCE; HIGH THROUGHPUT SCREENING; KINETICS; MOLECULE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; SCREENING;

ANIMALS; ANTHRACENES; CELL DIFFERENTIATION; CELLS, CULTURED; FIBROBLASTS; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; INDUCED PLURIPOTENT STEM CELLS; MICE; MICE, SCID; MICROSCOPY, FLUORESCENCE; MORPHOLINES; OCTAMER TRANSCRIPTION FACTOR-3; SOXB1 TRANSCRIPTION FACTORS;

EID: 84868272573     PISSN: 18735061     EISSN: 18767753     Source Type: Journal    
DOI: 10.1016/j.scr.2012.06.006     Document Type: Article

References (25)

1. Biel M., Wascholowski W., Giannis A. Epigenetics-an epicenter of gene regulation: histones and histone-modifying enzymes. Angew. Chem. Int. Ed. 2005, 44:3186-3216.
2. Bone H.K., Damiano T., Bartlett S., Perry A., Letchford J., Ripoll Y.S., Nelson A.S., Welham M.J. Involvement of GSK-3 in regulation of murine embryonic stem cell self-renewal revealed by a series of bisindolylmaleimides. Chem. Biol. 2009, 16:15-27.
3. Deng W. Induced pluripotent stem cells: paths to new medicines. A catalyst for disease modelling, drug discovery and regenerative therapy. EMBO Rep. 2010, 11:161-165.
4. Desbordes S.C., Placantonakis D.G., Ciro A., Socci N.D., Lee G., Djaballah H., Studer L. High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell 2008, 2:602-612.
5. Feng B., Ng J.H., Heng J.C., Ng H.H. Molecules that promote or enhance reprogramming of somatic cells to induced pluripotent stem cells. Cell Stem Cell 2009, 4:301-312.
6. Finnin M.S., Donigian J.R., Cohen A., Richon V.M., Rifkind R.A., Marks P.A., Breslow R., Pavletich N.P. Structures of a histone deacetylase homologue bound to the TSA and SAHA inhibitors. Nature 1999, 401:188-193.
7. Gonzalez R., Lee J.W., Snyder E.Y., Schultz P.G. Dorsomorphin promotes human embryonic stem cell self-renewal. Angew. Chem. Int. Ed. 2011, 50:3439-3441.
8. Huangfu D., Maehr R., Guo W., Eijkelenboom A., Snitow M., Chen A.E., Melton D.A. Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat. Biotechnol. 2008, 26:795-797.
9. Im C.N., Kang N.Y., Ha H.H., Bi X., Lee J.J., Park S.J., Lee S.Y., Vendrell M., Kim Y.K., Lee J.S., Li J., Ahn Y.H., Feng B., Ng H.H., Yun S.W., Chang Y.T. A fluorescent rosamine compound selectively stains pluripotent stem cells. Angew. Chem. Int. Ed. 2010, 49:7497-7500.
10. Judson R.L., Babiarz J.E., Venere M., Blelloch R. Embryonic stem cell-specific microRNAs promote induced pluripotency. Nat. Biotechnol. 2009, 27:459-461.
11. Kang N.Y., Yun S.W., Ha H.H., Park S.J., Chang Y.T. Embryonic and induced pluripotent stem cell staining and sorting with the live-cell fluorescence imaging probe CDy1. Nat. Protoc. 2011, 6:1044-1052.
12. Kim D., Kim C.H., Moon J.I., Chung Y.G., Chang M.Y., Han B.S., Ko S., Yang E., Cha K.Y., Lanza R., Kim K.S. Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins. Cell Stem Cell 2009, 4:472-476.
13. Lin T., Ambasudhan R., Yuan X., Li W., Hilcove S., Abujarour R., Lin X., Hahm H.S., Hao E., Hayek A., Ding S. A chemical platform for improved induction of human iPSCs. Nat. Meth. 2009, 6:805-808.
14. Lyssiotis C.A., Foreman R.K., Staerk J., Garcia M., Mathur D., Markoulaki S., Hanna J., Lairson L.L., Charette B.D., Bouchez L.C., Bollong M., Kunick C., Brinker A., Cho C.Y., Schultz P.G., Jaenisch R. Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4. Proc. Natl. Acad. Sci. U. S. A. 2009, 106:8912-8917.
15. Lyssiotis C.A., Lairson L.L., Boitano A.E., Wurdak H., Zhu S., Schultz P.G. Chemical control of stem cell fate and developmental potential. Angew. Chem. Int. Ed. 2011, 50:200-242.
16. Mikkelsen T.S., Hanna J., Zhang X., Ku M., Wernig M., Schorderet P., Bernstein B.E., Jaenisch R., Lander E.S., Meissner A. Dissecting direct reprogramming through integrative genomic analysis. Nature 2008, 454:49-55.
17. Nakagawa M., Koyanagi M., Tanabe K., Takahashi K., Ichisaka T., Aoi T., Okita K., Mochiduki Y., Takizawa N., Yamanaka S. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat. Biotechnol. 2008, 26:101-106.
18. Okita K., Ichisaka T., Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007, 448:313-317.
19. Rubin L.L. Stem cells and drug discovery: the beginning of a new era?. Cell 2008, 132:549-552.
20. Shi Y., Do J.T., Desponts C., Hahm H.S., Scholer H.R., Ding S. A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2008, 2:525-528.
21. Shi Y., Desponts C., Do J.T., Hahm H.S., Scholer H.R., Ding S. Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 2008, 3:568-574.
22. Takahashi K., Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006, 126:663-676.
23. Warren L., Manos P.D., Ahfeldt T., Loh Y.H., Li H., Lau F., Ebina W., Mandal P.K., Smith Z.D., Meissner A., Daley G.Q., Brack A.S., Collins J.J., Cowan C., Schlaeger T.M., Rossi D.J. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 2010, 7:618-630.
24. Xu Y., Shi Y., Ding S. A chemical approach to stem-cell biology and regenerative medicine. Nature 2008, 453:338-344.
25. Zhu S., Li W., Zhou H., Wei W., Ambasudhan R., Lin T., Kim J., Zhang K., Ding S. Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell Stem Cell 2010, 7:651-655.