Glycosylatable GFP as a compartment-specific membrane topology reporter

Biochemical and Biophysical Research Communications

Volumn 427, Issue 4, 2012, Pages 780-784

  Lee, Hunsang ^a   Min, Jisoo ^a   von Heijne, Gunnar ^b   Kim, Hyun ^a  

^a Seoul National University   (South Korea)

^b STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

GFP; Membrane topology; N linked glycosylation; Saccharomyces cerevisiae; Yeast

Indexed keywords

GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LYSATE; CYTOSOL; ENDOPLASMIC RETICULUM; FLUORESCENCE ANALYSIS; GLYCOSYLATION; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; WHOLE CELL; YEAST;

AMINO ACID SEQUENCE; ANIMALS; CELL MEMBRANE; CYTOSOL; ENDOPLASMIC RETICULUM; FLUORESCENCE; GLYCOSYLATION; GREEN FLUORESCENT PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 84868206086     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.09.138     Document Type: Article

References (23)

1. Bernsel A., Viklund H., Hennerdal A., Elofsson A. TOPCONS: consensus prediction of membrane protein topology. Nucleic Acids Res. 2009, 37:W465-W468.
2. Granseth E., Viklund H., Elofsson A. ZPRED: predicting the distance to the membrane center for residues in alpha-helical membrane proteins. Bioinformatics 2006, 22:e191-e196.
3. Viklund H., Bernsel A., Skwark M., Elofsson A. SPOCTOPUS: a combined predictor of signal peptides and membrane protein topology. Bioinformatics 2008, 24:2928-2929.
4. Viklund H., Elofsson A. Best alpha-helical transmembrane protein topology predictions are achieved using hidden Markov models and evolutionary information. Protein Sci. 2004, 13:1908-1917.
5. Kim H., Melen K., von Heijne G. Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions. J. Biol. Chem. 2003, 278:10208-10213.
6. Rapp M., Drew D., Daley D.O., Nilsson J., Carvalho T., Melen K., De Gier J.W., Von Heijne G. Experimentally based topology models for E. coli inner membrane proteins. Protein Sci. 2004, 13:937-945.
7. Kim H., Melen K., Osterberg M., von Heijne G. A global topology map of the Saccharomyces cerevisiae membrane proteome. Proc. Natl. Acad. Sci. USA 2006, 103:11142-11147.
8. Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Global topology analysis of the Escherichia coli inner membrane proteome. Science 2005, 308:1321-1323.
9. Green N., Fang H., Kalies K.U., Canfield V. Determining the topology of an integral membrane protein. Curr. Protoc. Cell Biol. 2001, (Chapter 5, Unit5.2).
10. Ter Horst R., Lolkema J.S. Rapid screening of membrane topology of secondary transport proteins. Biochim. Biophys. Acta 2010, 1798:672-680.
11. Drew D.E., von Heijne G., Nordlund P., de Gier J.W. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett. 2001, 507:220-224.
12. Lorenz H., Hailey D.W., Lippincott-Schwartz J. Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization. Nat. Methods 2006, 3:205-210.
13. Deak P.M., Wolf D.H. Membrane topology and function of Der3/Hrd1p as a ubiquitin protein ligase (E3) involved in endoplasmic reticulum degradation. J. Biol. Chem. 2001, 276:10663-10669.
14. Lundin C., Kim H., Nilsson I., White S.H., von Heijne G. Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for N(in)-C(out) and N(out)-C(in) transmembrane helices. Proc. Natl. Acad. Sci. USA 2008, 105:15702-15707.
15. Cormack B.P., Bertram G., Egerton M., Gow N.A., Falkow S., Brown A.J. Yeast-enhanced green fluorescent protein (yEGFP)a reporter of gene expression in Candida albicans. Microbiology 1997, 143(Pt 2):303-311.
16. Oldenburg K.R., Vo K.T., Michaelis S., Paddon C. Recombination-mediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res. 1997, 25:451-452.
17. Mumberg D., Muller R., Funk M. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 1995, 156:119-122.
18. Newstead S., Kim H., von Heijne G., Iwata S., Drew D. High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 2007, 104:13936-13941.
19. Hessa T., Reithinger J.H., von Heijne G., Kim H. Analysis of transmembrane helix integration in the endoplasmic reticulum in S. cerevisiae. J. Mol. Biol. 2009, 386:1222-1228.
20. Sung M.K., Huh W.K. In vivo quantification of protein-protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay. J. Microbiol. Methods 2010, 83:194-201.
21. Yang F., Moss L.G., Phillips G.N. The molecular structure of green fluorescent protein. Nat. Biotechnol. 1996, 14:1246-1251.
22. Wolfe P.B., Wickner W., Goodman J.M. Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope. J. Biol. Chem. 1983, 258:12073-12080.
23. Suzuki N., Hiraki M., Yamada Y., Matsugaki N., Igarashi N., Kato R., Dikic I., Drew D., Iwata S., Wakatsuki S., Kawasaki M. Crystallization of small proteins assisted by green fluorescent protein. Acta Crystallogr. D Biol. Crystallogr. 2010, 66:1059-1066.