Selection of Suitable Housekeeping Genes for Real-Time Quantitative PCR in CD4+ Lymphocytes from Asthmatics with or without Depression

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Wang, Ting ^a   Liang, Zong An ^a   Sandford, Andrew J ^b   Xiong, Xing Yu ^a   Yang, Yin Yin ^a   Ji, Yu Lin ^a   He, Jian Qing ^a  

^a SICHUAN UNIVERSITY   (China)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; BETA ACTIN; BETA GLUCURONIDASE; COMPLEMENTARY DNA; CYCLOPHILIN A; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; PHOSPHOGLYCERATE KINASE; PHOSPHOGLYCERATE KINASE 1; RIBOSOMAL PROTEIN L13A; RIBOSOME PROTEIN; RNA 28S; SALBUTAMOL; UNCLASSIFIED DRUG;

ADULT; ARTICLE; ASTHMA; ASTHMATIC STATE; ATOPY; CD4+ T LYMPHOCYTE; CELL ISOLATION; CLINICAL ARTICLE; COMORBIDITY; CONTROLLED STUDY; DEPRESSION; FEMALE; FORCED EXPIRATORY VOLUME; FORCED VITAL CAPACITY; GENE EXPRESSION; GENE EXPRESSION PROFILING; HAMILTON SCALE; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; MALE; NUCLEOTIDE SEQUENCE; PRICK TEST; QUALITY OF LIFE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION;

ADULT; ASTHMA; CD4-POSITIVE T-LYMPHOCYTES; DEPRESSION; FEMALE; FLOW CYTOMETRY; GENES, ESSENTIAL; HUMANS; MALE; REAL-TIME POLYMERASE CHAIN REACTION; YOUNG ADULT;

EID: 84868152467     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0048367     Document Type: Article

References (28)

1. Scott KM, Von Korff M, Ormel J, Zhang MY, Bruffaerts R, et al. (2007) Mental disorders among adults with asthma: results from the World Mental Health Survey. Gen Hosp Psychiatry 29: 123-133.
2. Lavoie KL, Bacon SL, Barone S, Cartier A, Ditto B, et al. (2006) What is worse for asthma control and quality of life: depressive disorders, anxiety disorders, or both? Chest 130: 1039-1047.
3. Van Lieshout RJ, Bienenstock J, MacQueen GM, (2009) A Review of Candidate Pathways Underlying the Association Between Asthma and Major Depressive Disorder. Psychosomatic Medicine 71: 187-195.
4. Corrigan CJ, Hartnell A, Kay AB, (1988) T lymphocyte activation in acute severe asthma. Lancet 1: 1129-1132.
5. Gonzalez MC, Diaz P, Galleguillos FR, Ancic P, Cromwell O, et al. (1987) Allergen-induced recruitment of bronchoalveolar helper (OKT4) and suppressor (OKT8) T-cells in asthma. Relative increases in OKT8 cells in single early responders compared with those in late-phase responders. The American review of respiratory disease 136: 600-604.
6. Robinson DS (2010) The role of the T cell in asthma. J Allergy Clin Immunol 126: 1081-1091; quiz 1092-1083.
7. Miller AH, (2010) Depression and immunity: a role for T cells? Brain Behav Immun 24: 1-8.
8. Bustin SA, (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology 25: 169-193.
9. Hamilton M, (1960) A rating scale for depression. Journal of neurology, neurosurgery, and psychiatry 23: 56-62.
10. Schatz M, Sorkness CA, Li JT, Marcus P, Murray JJ, et al. (2006) Asthma Control Test: reliability, validity, and responsiveness in patients not previously followed by asthma specialists. The Journal of allergy and clinical immunology 117: 549-556.
11. Juniper EF, Buist AS, Cox FM, Ferrie PJ, King DR, (1999) Validation of a standardized version of the Asthma Quality of Life Questionnaire. Chest 115: 1265-1270.
12. Mane VP, Heuer MA, Hillyer P, Navarro MB, Rabin RL, (2008) Systematic Method for Determining an Ideal Housekeeping Gene for Real-Time PCR Analysis. J Biomol Tech 19: 342-347.
13. Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP, (2004) Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol Lett 26: 509-515.
14. Andersen CL, Jensen JL, Orntoft TF, (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64: 5245-5250.
15. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3: RESEARCH0034.
16. Irwin MR, Miller AH, (2007) Depressive disorders and immunity: 20 years of progress and discovery. Brain Behav Immun 21: 374-383.
17. Handschumacher R, Harding M, Rice J, Drugge R, Speicher D, (1984) Cyclophilin: a specific cytosolic binding protein for cyclosporin A. Science. 226: 544-547.
18. Colgan J, Asmal M, Neagu M, Yu B, Schneidkraut J, et al. (2004) Cyclophilin A regulates TCR signal strength in CD4+ T cells via a proline-directed conformational switch in Itk. Immunity 21: 189-201.
19. Arora K, Gwinn WM, Bower MA, Watson A, Okwumabua I, et al. (2005) Extracellular cyclophilins contribute to the regulation of inflammatory responses. J Immunol 175: 517-522.
20. Damsker JM, Bukrinsky MI, Constant SL, (2007) Preferential chemotaxis of activated human CD4+ T cells by extracellular cyclophilin A. J Leukoc Biol. 82: 613-618.
21. Balsley MA, Malesevic M, Stemmy EJ, Gigley J, Jurjus RA, et al. (2010) A cell-impermeable cyclosporine A derivative reduces pathology in a mouse model of allergic lung inflammation. J Immunol 185: 7663-7670.
22. Stemmy EJ, Balsley MA, Jurjus RA, Damsker JM, Bukrinsky MI, et al. (2011) Blocking cyclophilins in the chronic phase of asthma reduces the persistence of leukocytes and disease reactivation. Am J Respir Cell Mol Biol 45: 991-998.
23. van den Toorn LM, Overbeek SE, de Jongste JC, Leman K, Hoogsteden HC, et al. (2001) Airway inflammation is present during clinical remission of atopic asthma. Am J Respir Crit Care Med 164: 2107-2113.
24. Humbles AA, Conroy DM, Marleau S, Rankin SM, Palframan RT, et al. (1997) Kinetics of eotaxin generation and its relationship to eosinophil accumulation in allergic airways disease: analysis in a guinea pig model in vivo. Journal of Experimental Medicine 186: 601-612.
25. Gonzalo JA, Lloyd CM, Kremer L, Finger E, Martinez AC, et al. (1996) Eosinophil recruitment to the lung in a murine model of allergic inflammation. The role of T cells, chemokines, and adhesion receptors. J Clin Invest 98: 2332-2345.
26. He JQ, Sandford AJ, Wang IM, Stepaniants S, Knight DA, et al. (2008) Selection of housekeeping genes for real-time PCR in atopic human bronchial epithelial cells. Eur Respir J 32: 755-762.
27. Oosthuizen JL, Gomez P, Ruan J, Hackett TL, Moore MM, et al. (2011) Dual organism transcriptomics of airway epithelial cells interacting with conidia of Aspergillus fumigatus. Plos One 6: e20527.
28. Fonceca AM, Flanagan BF, Trinick R, Smyth RL, McNamara PS, (2012) Primary airway epithelial cultures from children are highly permissive to respiratory syncytial virus infection. Thorax 67: 42-48.