Single nucleotide analysis of cytosine methylation by whole-genome shotgun bisulfite sequencing

Current Protocols in Molecular Biology

Volumn 1, Issue SUPPL.99, 2012, Pages

  Johnson, Michelle D ^a   Mueller, Michael ^a   Game, Laurence ^b   Aitman, Timothy J ^b  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Bisulfite sequencing; DNA methylation; Methylation profiling; Read mapping; Second generation sequencing

Indexed keywords

BISULFITE; CYTOSINE; DNA FRAGMENT; DOUBLE STRANDED DNA; GENOMIC DNA; NUCLEOTIDE; SINGLE STRANDED DNA; SULFITE;

AGAR GEL ELECTROPHORESIS; ARTICLE; CLINICAL PROTOCOL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA DENATURATION; DNA PURIFICATION; EXPERIMENTAL DESIGN; GC RICH SEQUENCE; GENE MAPPING; GENOME ANALYSIS; METHYLATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; REACTION TIME; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; CHEMISTRY; COMPUTER PROGRAM; DNA METHYLATION; DNA SEQUENCE; GENE LIBRARY; GENETIC PROCEDURES; GENOME; HUMAN; METABOLISM; METHODOLOGY;

CYTOSINE; DNA METHYLATION; GENE LIBRARY; GENETIC TECHNIQUES; GENOME; HUMANS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SOFTWARE; SULFITES;

EID: 84868134071     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb2123s99     Document Type: Article

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