Confocal laser scanning microscopic photoconversion: A new method to stabilize fluorescently labeled cellular elements for electron microscopic analysis

Current Protocols in Neuroscience

Volumn 1, Issue SUPPL.58, 2012, Pages

  Colello, Raymond J ^a   Tozer, Jordan ^a   Henderson, Scott C ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

Confocal microscopy; Diaminobenzidine; Electron microscopy; Immunolabeling; Photo oxidation; Ultrastructure

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; ASTROCYTE; CELL LABELING; CELL SELECTION; CLINICAL PROTOCOL; CONFOCAL LASER MICROSCOPY; ELECTRON MICROSCOPY; EXPERIMENTAL DESIGN; INTERMETHOD COMPARISON; LIGHT SCATTERING; NONHUMAN; PHOTOOXIDATION; PRIORITY JOURNAL; RAT; REACTION TIME; STRUCTURE ANALYSIS; ANIMAL; BRAIN; CONFOCAL MICROSCOPY; CYTOLOGY; FLUORESCENCE MICROSCOPY; METABOLISM; METHODOLOGY; NERVE CELL; SPRAGUE DAWLEY RAT; STAINING; ULTRASTRUCTURE;

NERVE PROTEIN;

ANIMALS; BRAIN; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; NERVE TISSUE PROTEINS; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; STAINING AND LABELING;

EID: 84868101532     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.ns0215s58     Document Type: Article

References (11)

1. Burghardt, R.C. and Droleskey, R. 2006. Transmission electron microscopy. Curr. Protoc. Microbiol. 3:2B.1.1-2B.1.39.
2. Deerinck, T.J., Martone,M.E., Lev-Ram,V.,Green, D.P., Tsien, R.Y., Spector, D.L., Huang, S., and Ellisman, M.H. 1994. Fluorescence photooxidation with eosin: A method for high resolution immunolocalization an in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126:901-910.
3. De-Miguel, F.F., Muller, K.J., Adams, W.B., and Nicholls, J.G. 2002. Axotomy of single fluorescent nerve fibers in developing mammalian spinal cord by photoconversion of diaminobenzidine. J. Neursci. Methods 117:73-79.
4. Donovan, J. and Brown, P. 1998. Anesthesia. Curr. Protoc. Immunol. 27:1.4.1-1.4.5.
5. Kacza, J., Hartig,W., and Seeger, J. 1997. Oxygenenriched photoconversion of fluorescent dyes by means of a closed conversion chamber. J. Neursci. Methods 71:225-232.
6. Lubke, J. 1993. Photoconversion of diaminobenzidine with different fluorescent neuronal markers into a light and electron microscopic dense reaction product. Microsc. Res. Tech. 24:2-14.
7. Maranto, A. 1982. Neuronal mapping: A photooxidation of reaction makes Lucifer yellow useful for electron microscopy. Science 217:953-955.
8. Mark, M., Teletin, M., Antal, C., Wendling, O., Auwerx, J., Heikkinen, S., Khetchoumian, K., Argmann, C.A., and Dgheem, M. 2007. Histopathology in mouse metabolic investigations. Curr. Protoc. Mol. Biol. 78:29B.4.1-29B.4.32.
9. Sandell, J.H. and Masland, R.H. 1988. Photoconversion of some fluorescent markers to adiaminobenzidine product. J. Histochem. Cytochem. 36:555-559.
10. Singleton, C.D. and Casagrande, V.A. 1996. A reliable and sensitive method for fluorescent photoconversion. J. Neursci. Methods 64:47-54.
11. Tozer, J.T., Henderson, S.C., Sun, D., and Colello, R.J. 2007. Photoconversion using confocal laser scanning microscopy: a new tool for the ultrastructural analysis of fluorescently labeled cellular elements. J. Neursci. Methods 164:240-246.