Photoconversion using confocal laser scanning microscopy: A new tool for the ultrastructural analysis of fluorescently labeled cellular elements

Journal of Neuroscience Methods

Volumn 164, Issue 2, 2007, Pages 240-246

  Tozer, Jordan T ^a   Henderson, Scott C ^a   Sun, Dong ^a   Colello, Raymond J ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

Confocal laser scanning microscopy; Diaminobenzidine; Fluorescence immunolabeling; Photoconversion; Transmission electron microscopy

Indexed keywords

DIAMINOBENZIDINE; FLUORESCENT DYE;

ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BRAIN SLICE; CHEMICAL REACTION; COCULTURE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; FILTER; FLUORESCENCE; HELIUM NEON LASER; IMAGE ANALYSIS; LIGHT; MICROSCOPE; NONHUMAN; OLIGODENDROGLIA; PHOTOCONVERSION; PRIORITY JOURNAL; RAT; REACTION ANALYSIS; REACTION TIME; SPINAL GANGLION; SPRAGUE DAWLEY RAT; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; BRAIN; COCULTURE TECHNIQUES; EMBRYO; FLUORESCENT DYES; GANGLIA, SPINAL; GLIAL FIBRILLARY ACIDIC PROTEIN; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, TRANSMISSION; NERVE TISSUE PROTEINS; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; ULTRAVIOLET RAYS;

EID: 34347340503     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.05.004     Document Type: Article

References (10)

1. Deerinck T.J., et al. Fluorescence photooxidation with eosin: a method for high resolution immunolocalization an in situ hybridization detection for light and electron microscopy. J Cell Biol 126 (1994) 901-910
2. De-Miguel F.F., Muller K.J., Adams W.B., and Nicholls J.G. Axotomy of single fluorescent nerve fibers in developing mammalian spinal cord by photoconversion of diaminobenzidine. J Neurosci Methods 117 (2002) 73-79
3. Kacza J., Hartig W., and Seeger J. Oxygen-enriched photoconversion of fluorescent dyes by means of a closed conversion chamber. J Neurosci Methods 71 (1997) 225-232
4. Lubke J. Photoconversion of diaminobenzidine with different fluorescent neuronal markers into a light and electron microscopic dense reaction product. Microsc Res Tech 24 (1993) 2-14
5. Maranto A. Neuronal mapping: a photooxidation of reaction makes Lucifer yellow useful for electron microscopy. Science 217 (1982) 953-955
6. McCarty K.D., and DeVellis J. Preparation of separate astroglial and oligodendroglial cultures from rat cerebral tissue. J Cell Biol 85 (1980) 890-902
7. Sandell J.H., and Masland R.H. Photoconversion of some fluorescent markers to a diaminobenzidine product. J Histochem Cytochem 36 (1988) 555-559
8. Sharma K.V., and Bigbee J.W. Acetylcholinesterase antibody treatment results in neurite detachment and reduced outgrowth from cultured neurons: further evidence for a cell adhesive role for neuronal acetylcholinesterase. J Neurosci 53 (1998) 454-464
9. Singleton C.D., and Casagrande V.A. A reliable and sensitive method for fluorescent photoconversion. J Neurosci Methods 64 (1996) 47-54
10. Sun D., McGinn M.J., Zhou Z., Harvey H.B., Bullock M.R., and Colello R.J. Anatomical integration of newly generated dentate granule neurons following traumatic brain injury in adult rats and its association to cognitive recovery. Exp Neurol 204 (2007) 264-272