A reliable and sensitive method for fluorescent photoconversion

Journal of Neuroscience Methods

Volumn 64, Issue 1, 1996, Pages 47-54

  Singleton, C D ^a   Casagrande, V A ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Diaminobenzidine; DiI; Giemsa stain; Intensification; Macaque monkey; Nitro blue tetrazolium; Visual cortex

Indexed keywords

FLUORESCENT DYE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FETUS; HISTOCHEMISTRY; MONKEY; NONHUMAN; PRIORITY JOURNAL; VISUAL CORTEX;

EID: 0030049827     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(95)00097-6     Document Type: Article

References (15)

1. Adams, J.C. (1981) Heavy metal intensification of DAB-based HRP reaction product, J. Histochem. Cytochem., 29: 775.
2. Bartheld, C.S., Cunningham, D.E. and Rubel, E.W (1990) Neuronal tracing with DiI: decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis, J. Histochem. Cytochem., 38: 725-733.
3. Bentivoglio, M. and Su, H.S. (1990) Photoconversion of fluorescent retrograde tracers, Neurosci. Lett., 113: 127-133.
4. Casagrande, V.A. and Hutchins, J.B. (1990) Methods for analyzing neuronal connections in mammals, Methods Neurosci., 3: 188-207.
5. Iniguez, C., Gayoso, M.J. and Carreres, J. (1985) A versatile and simple method for staining nervous tissue using giemsa dye, J. Neurosci. Methods, 13: 77-86.
6. Linke, R. and Frotscher, M. (1993) Development of the rat septohippocampal projection: tracing with DiI and electron microscopy of identified growth cones. J. Comp. Neurol., 332: 69-88.
7. Lubke, J. (1993a) Photoconversion of diaminobenzidine with different fluorescent neuronal markers into a light and electron microscopic dense reaction product, Microsc. Res. Tech., 24: 2-14.
8. Lubke, J. (1993b) Photoconversion of different fluorescent substances for light and electron microscopy, Neurosci. Protocols, 50: 601-613.
9. Lund, J.S., Boothe, R.G. and Lund, R.D. (1977) Development of neurons in the visual cortex (area 17) of the monkey (Macaca nemestrina): a Golgi study from fetal day 127 to postnatal maturity, J. Comp. Neurol., 176: 149-188.
10. Maranto, A.R. (1982) Neuronal mapping: a photooxidation reaction makes lucifer yellow useful for electron microscopy, Science, 217: 953-955.
11. Papadopoulos, G.C. and Dori, I. (1993) DiI labeling combined with conventional immunocytochemical techniques for correlated light and electron microscopic studies, J. Neurosci. Methods, 46: 251-258.
12. Sandell, J.H., and Masland, R.H. (1988) Photoconversion of some fluorescent markers to a diaminobenzidine product, J. Histochem. Cytochem., 36: 555-559.
13. Schmued, L.C. and Snavely, L.F. (1993) Photoconversion and electron microscopic localization of the fluorescent axon tracer fluoro-ruby (rhodamine-dextran-amine), J. Histochem. Cytochem., 41: 777-782.
14. Supprian, T., Senitz, D. and Beckmann, H. (1993) Presentation of human neocortical neurons stained with the carbocyanine dye DiI compared to the golgi silver impregnation technique, J. Hirnforsch., 34: 403-406.
15. Vaney, D.I. (1992) Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: applications for intracellular labelling and immunohistochemistry, J. Neurosci. Methods, 44: 217-223.