An improved bioluminescence-based signaling assay for odor sensing with a yeast expressing a chimeric olfactory receptor

Biotechnology and Bioengineering

Volumn 109, Issue 12, 2012, Pages 3143-3151

  Fukutani, Yosuke ^a   Ishii, Jun ^b   Noguchi, Keiichi ^a   Kondo, Akihiko ^b   Yohda, Masafumi ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

Biosensing; G protein coupled receptor; Luc reporter gene assay; Olfactory receptor; Saccharomyces cerevisiae

Indexed keywords

ASSAY SYSTEM; BIOSENSING; FIREFLY LUCIFERASE; G PROTEIN; G PROTEIN COUPLED RECEPTORS; ODOR SENSING; ODOR SENSORS; OLFACTORY RECEPTORS; REPORTER ASSAY; REPORTER GENE ASSAYS; YEAST STRAIN;

BIOLUMINESCENCE; BIOMIMETICS; GENES; PHOSPHORESCENCE; SENSORS;

YEAST;

G PROTEIN COUPLED RECEPTOR; GENOMIC DNA; LUCIFERASE; PHEROMONE RECEPTOR;

ARTICLE; BIOENGINEERING; BIOLUMINESCENCE; BIOSENSOR; ELECTRON MICROSCOPY; GENE EXPRESSION; IMMUNOBLOTTING; NONHUMAN; ODOR; OLFACTORY RECEPTOR; POLYMERASE CHAIN REACTION; PROMOTER REGION; REPORTER GENE; SACCHAROMYCES CEREVISIAE; YEAST; YEAST CELL;

BIOTECHNOLOGY; DINITROBENZENES; GTP-BINDING PROTEIN ALPHA SUBUNITS; IMMUNOHISTOCHEMISTRY; LUCIFERASES, FIREFLY; LUMINESCENT MEASUREMENTS; ODORS; PROMOTER REGIONS, GENETIC; RECEPTORS, ODORANT; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION;

SACCHAROMYCES CEREVISIAE;

EID: 84868094066     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24589     Document Type: Article

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