Expression of ezrin correlates with malignant phenotype of lung cancer, and in vitro knockdown of ezrin reverses the aggressive biological behavior of lung cancer cells

Tumor Biology

Volumn 33, Issue 5, 2012, Pages 1493-1504

  Wang, Endi ^b   Li, Qingchang ^a   Gao, Hui ^a   Xu, Hongtao ^a   Wang, Xin ^a   Pan, Yongqi ^a   Hao, Fengxia ^a   Qiu, Xueshan ^a   Stoecker, Maggie ^b   Wang, Enhua ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

^b DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Cell adhesion; Ezrin; Lung cancer; Metastasis; Tumor progression

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BETA CATENIN; EZRIN; MESSENGER RNA; SMALL INTERFERING RNA; UVOMORULIN;

APICAL MEMBRANE; ARTICLE; BRONCHUS MUCOSA; CANCER CELL CULTURE; CANCER STAGING; CELL ASSAY; CELL INVASION; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; LUNG CANCER; LUNG CARCINOGENESIS; LUNG PARENCHYMA; LYMPH NODE METASTASIS; MAJOR CLINICAL STUDY; MIGRATION INHIBITION; MITOSIS INHIBITION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION; WESTERN BLOTTING; WOUND HEALING;

EID: 84868090394     PISSN: 10104283     EISSN: 14230380     Source Type: Journal    
DOI: 10.1007/s13277-012-0400-9     Document Type: Article

References (36)

1. Berghmans T, Dusart M, Paesmans M, Hossein-Foucher C, Buvat I, Castaigne C. Primary tumor standardized uptake value (SUVmax) measured on fluorodeoxyglucose positron emission tomography (FDG-PET) is of prognostic value for survival in non-small cell lung cancer (NSCLC): a systematic review and meta-analysis (MA) by the European Lung Cancer Working Party for the IASLC Lung Cancer Staging Project. J Thorac Oncol. 2008;3:6-12.
2. Shibue T, Weinberg RA. Metastatic colonization: settlement, adaptation and propagation of tumor cells in a foreign tissue environment. Semin Cancer Biol. 2011;21:99-106.
3. Lallemand D, Arpin M. Moesin/ezrin: a specific role in cell metastasis? Pigm Cell Melanoma R. 2010;23:6-7.
4. Neisch AL, Fehon RG. Ezrin, Radixin and Moesin: key regulators of membrane-cortex interactions and signaling. Curr Opin Cell Biol. 2011;23:377-82.
5. Huang HY, Li CF, Fang FM, Tsai JW, Li SH, Lee YT, et al. Prognostic implication of ezrin overexpression in myxofibrosarcomas. Ann Surg Oncol. 2010;17:3212-9.
6. Xie JJ, Xu LY, Xie YM, Zhang HH, Cai WJ, Zhou F, et al. Roles of ezrin in the growth and invasiveness of esophageal squamous carcinoma cells. Int J Cancer. 2009;124:2549-58.
7. Ohtani K, Sakamoto H, Rutherford T, Chen Z, Kikuchi A, Yamamoto T, et al. Ezrin, a membrane-cytoskeletal linking protein, is highly expressed in atypical endometrial hyperplasia and uterine endometrioid adenocarcinoma. Cancer Lett. 2002;179:79-86.
8. Khanna C, Wan X, Bose S, Cassaday R, Olomu O, Mendoza A, et al. The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat Med. 2004;10:182-6.
9. Ana C, Bendahl PO, Åkerman M, Domanski HA, Rydholm A, Engellau J, et al. Ezrin expression predicts local recurrence and development of metastases in soft tissue sarcomas. J Clin Pathol. 2011;64:689-94.
10. Hunter KW. Ezrin, a key component in tumor metastasis. Trends Mol Med. 2004;10:201-4.
11. Kocher HM, Sandle J, Mirza TA, Li NF, Hart IR. Ezrin interacts with cortactin to form podosomal rosettes in pancreatic cancer cells. Gut. 2009;58:271-84.
12. Osawa H, Smith CA, Ra YS, Kongkham P, Rutka JT. The role of the membrane cytoskeleton cross-linker ezrin in medulloblastoma cells. Neuro-Oncology. 2009;11:381-93.
13. Ohtani K, Sakamoto H, Rutherford T, Chen Z, Satoh K, Naftolin F. Ezrin, a membrane-cytoskeletal linking protein, is involved in the process of invasion of endometrial cancer cells. Cancer Lett. 1999;147:31-8.
14. Martin M, Andreoli C, Sahuquet A, Montcourrier P, Algrain M, Mangeat P. Ezrin Nh2-terminal domain inhibits the cell extension activity of the cooh-terminal domain. J Cell Biol. 1995;128:1081-93.
15. Li Q, Wu M, Wang H, Xu G, Zhu T, Zhang Y, et al. Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells. Cancer Lett. 2008;261:55-63.
16. Helander TS, Carpen O, Turunen O, Kovanen PE, Vaheri A, Timonen T. ICAM-2 redistributed by ezrin as a target for killer cells. Nature. 1996;382:265-8.
17. Pujuguet P, Maestro LD, Gautreau A, Louvard D, Arpin M. Ezrin regulates E-cadherin-dependent adherens junction assembly through Rac1 activation. Mol Biol Cell. 2003;14:2181-91.
18. Allenspach EJ, Cullinan P, Tong J, Tang Q, Tesciuba AG, Cannon JL, et al. ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse. Immunity. 2001;15:739-50.
19. Mackay DJG, Esch F, Furthmayr H, Hall A. Rho-and Rac-dependent assembly of focal adhesion complexes and actin filaments in permeabilized fibroblasts: an essential role for ezrin/radixin/moesin proteins. Cell Biol. 1997;138:927-38.
20. Stratton MR. Exploring the genomes of cancer cells: progress and promise. Science. 2011;331:1553-8.
21. William NJ, Glisson BS. Novel strategies for the treatment of small-cell lung carcinoma. Nat Rev Clin Oncol. 2011;8:611-9.
22. Zhang XQ, Chen GP, Wu T, Yan JP, Zhou JY. Expression and clinical significance of ezrin in non-small-cell lung cancer. Clin Lung Cancer. 2011 Dec 1. doi: 10.1016/j.cllc.2011.04.002.
23. Bretscher A, Reczek D, Berryman M. Ezrin: a protein requiring conformational activation to link microfilaments to the plasma membrane in the assembly of cell surface structures. J Cell Sci. 1997;110:3011-8.
24. Chen L, Hughes RA, Baines AJ, Conboy J, Mohandas N, An X. Protein 4.1R regulates cell adhesion, spreading, migration and motility of mouse keratinocytes by modulating surface expression of beta 1 integrin. J Cell Sci. 2011;124:2478-87.
25. Yu Y, Khan J, Khanna C, Helman L, Meltzer PS, Merlino G. Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat Med. 2004;10:175-81.
26. Ren L, Hong SH, Cassavaugh J, Osborne T, Chou AJ, Kim SY, et al. The actin-cytoskeleton linker protein ezrin is regulated during osteosarcoma metastasis by PKC. Oncogene. 2009;28:792-802.
27. Estecha A, Sanchez-Martin L, Puig-Kroeger A, Bartolome RA, Teixido J, Samaniego RL, et al. Moesin orchestrates cortical polarity of melanoma tumour cells to initiate 3D invasion. J Cell Sci. 2009;122:3492-501.
28. Tynninen O, Carpen O, Jaaskelainen J, Paavonen T, Paetau A. Ezrin expression in tissue microarray of primary and recurrent gliomas. Neuropathol Appl Neuro. 2004;30:472-7.
29. Takeichi M, Watabe M, Shibamoto S, Ito F, Oda H, Uemura T, et al. Dynamic control of cell-cell adhesion for multicellular organization. C R Acad Sci III. 1993;316:818-21.
30. Nagafuchi A, Ishihara S, Tsukita S. The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules. J Biol Chem. 1994;127:235-45.
31. Federici C, Brambilla D, Lozupone F, Matarrese P, de Milito A, Lugini L, et al. Pleiotropic function of ezrin in human metastatic melanomas. Int J Cancer. 2009;124:2804-12.
32. Lugini L, Matarrese P, Tinari A, Lozupone F, Federici C, Iessi E, et al. Cannibalism of live lymphocytes by human metastatic but not primary melanoma cells. Cancer Res. 2006;66:3629-38.
33. Brambilla D, Zamboni S, Federici C, Lugini L, Lozupone F, Milito AD, Cecchetti S, Cianfriglia M, Fais S. P-glycoprotein binds to ezrin at amino acid residues 149-242 in the FERM domain and plays a key role in the multidrug resistance of human osteosarcoma. Int J Cancer. 2011, doi: 10.1002/ijc.26285.
34. Parlato S, Giammarioli AM, Logozzi M, Lozupone F, Matarrese P, Luciani F, et al. CD95 (APO-1/Fas) linkage to the actin cytoskeleton through ezrin in human T lymphocytes: a novel regulatory mechanism of the CD95 apoptotic pathway. EMBO J. 2000;19:5123-34.
35. Fais S. Moulding the shape of a metastatic cell. Leuk Res. 2010;34:843-7.
36. Brambilla D, Fais S. The Janus-faced role of ezrin in linking cells to either normal or metastatic phenotype. Int J Cancer. 2009;125:2239-45.