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Volumn 26, Issue 12, 2012, Pages 1488-1493

Purification of monoclonal antibodies, IgG1, from cell culture supernatant by use of metal chelate convective interaction media monolithic columns

Author keywords

Cell culture supernatant; CIM disk; Metal chelate affinity chromatography; Mouse monoclonal antibody; Purification

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELLS; CHELATION; MAMMALS; METAL IONS; MONOCLONAL ANTIBODIES; NITROGEN COMPOUNDS; PURIFICATION; SODIUM CHLORIDE; SULFUR COMPOUNDS; SURFACE PLASMON RESONANCE; ZINC COMPOUNDS;

EID: 84867736198     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.2721     Document Type: Article
Times cited : (11)

References (40)
  • 3
    • 48949117181 scopus 로고    scopus 로고
    • Methacrylate-based short monolithic columns: enabling tools for rapid and efficient analyses of biomolecules and nanoparticles
    • Barut M, Podgornik A, Urbas L, Gabor B, Brne P, Vidic J, Plevcak S and Strancar A. Methacrylate-based short monolithic columns: enabling tools for rapid and efficient analyses of biomolecules and nanoparticles. Journal of Separation Science 2008; 31(11): 1867-1880.
    • (2008) Journal of Separation Science , vol.31 , Issue.11 , pp. 1867-1880
    • Barut, M.1    Podgornik, A.2    Urbas, L.3    Gabor, B.4    Brne, P.5    Vidic, J.6    Plevcak, S.7    Strancar, A.8
  • 4
    • 0034578670 scopus 로고    scopus 로고
    • Comparison of antibody binding to immobilized groupspecific affinity ligands in high performance monolith affinity chromatography
    • Berruex LG, Freitag R and Tennikova TB. Comparison of antibody binding to immobilized groupspecific affinity ligands in high performance monolith affinity chromatography. Journal of Pharmaceutical and Biomedical Analysis 2000; 24: 95-104.
    • (2000) Journal of Pharmaceutical and Biomedical Analysis , vol.24 , pp. 95-104
    • Berruex, L.G.1    Freitag, R.2    Tennikova, T.B.3
  • 5
    • 0028935311 scopus 로고
    • Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography
    • Boden V, Winzerling JJ, Vijayalakshmi MA and Porath J. Rapid one-step purification of goat immunoglobulins by immobilized metal ion affinity chromatography. Journal of Immunological Methods 1994; 181: 225-232.
    • (1994) Journal of Immunological Methods , vol.181 , pp. 225-232
    • Boden, V.1    Winzerling, J.J.2    Vijayalakshmi, M.A.3    Porath, J.4
  • 6
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976; 72: 248-254.
    • (1976) Analytical Biochemistry , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 7
    • 77949918331 scopus 로고    scopus 로고
    • Evaluation of immobilized metal-ion affinity chromatography (IMAC) as a technique for igg1monoclonal antibodies purification: the effect of chelating ligand and support
    • Bresolin ITL, Ribeiro MB, Tamashiro WMSC, Augusto EFP, Vijayalakshm MA and Bueno SMA. Evaluation of immobilized metal-ion affinity chromatography (IMAC) as a technique for igg1monoclonal antibodies purification: the effect of chelating ligand and support. Applied Biochemistry and Biotechnology 2010; 160: 2148-2165.
    • (2010) Applied Biochemistry and Biotechnology , vol.160 , pp. 2148-2165
    • Bresolin, I.T.L.1    Ribeiro, M.B.2    Tamashiro, W.M.S.C.3    Augusto, E.F.P.4    Vijayalakshm, M.A.5    Bueno, S.M.A.6
  • 8
    • 33847039612 scopus 로고    scopus 로고
    • Fast and efficient separation of immunoglobulin M from immunoglobulin G using short monolithic columns
    • Brne P, Podgornik A, Bencina K, Gabor B, Strancar A and Peterka M. Fast and efficient separation of immunoglobulin M from immunoglobulin G using short monolithic columns. Journal of Chromatography. A 2006; 1144: 120-125.
    • (2006) Journal of Chromatography. A , vol.1144 , pp. 120-125
    • Brne, P.1    Podgornik, A.2    Bencina, K.3    Gabor, B.4    Strancar, A.5    Peterka, M.6
  • 10
    • 78651302173 scopus 로고    scopus 로고
    • Purification of monoclonal antibodies from cell-culture supernatant by use of anion exchange convective interaction media (CIM) monolithic columns
    • Dhivya AP, Kumar BP, Prasanna RR, Jayaprakash NS and Vijayalakshmi MA. Purification of monoclonal antibodies from cell-culture supernatant by use of anion exchange convective interaction media (CIM) monolithic columns. Chromatographia 2010; 72: 1183-1188.
    • (2010) Chromatographia , vol.72 , pp. 1183-1188
    • Dhivya, A.P.1    Kumar, B.P.2    Prasanna, R.R.3    Jayaprakash, N.S.4    Vijayalakshmi, M.A.5
  • 11
    • 0346219399 scopus 로고    scopus 로고
    • Factorial screening of antibody purification processes using three chromatography steps without protein A
    • Follman DK and Fahrner RL. Factorial screening of antibody purification processes using three chromatography steps without protein A. Journal of Chromatography. A 2004; 1024: 79-85.
    • (2004) Journal of Chromatography. A , vol.1024 , pp. 79-85
    • Follman, D.K.1    Fahrner, R.L.2
  • 13
    • 33745924699 scopus 로고    scopus 로고
    • Evaluation and comparison of alternatives to Protein A chromatography: mimetic and hydrophobic charge induction chromatographic stationary phases
    • Ghose S, Hubbard B and Cramer SM. Evaluation and comparison of alternatives to Protein A chromatography: mimetic and hydrophobic charge induction chromatographic stationary phases. Journal of Chromatography. A 2006; 1122: 144-152.
    • (2006) Journal of Chromatography. A , vol.1122 , pp. 144-152
    • Ghose, S.1    Hubbard, B.2    Cramer, S.M.3
  • 15
    • 0000844197 scopus 로고
    • Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography
    • Hemdan ES, Zhao YJ, Sulkowski E and Porath J. Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography. Biochemistry 1989; 86: 1811-1815.
    • (1989) Biochemistry , vol.86 , pp. 1811-1815
    • Hemdan, E.S.1    Zhao, Y.J.2    Sulkowski, E.3    Porath, J.4
  • 17
    • 0035975926 scopus 로고    scopus 로고
    • Application of monoliths as supports for affinity chromatography and fast enzymatic conversion
    • Josic D and Buchacher A. Application of monoliths as supports for affinity chromatography and fast enzymatic conversion. Journal of Biochemical and Biophysical Methods 2006; 49: 153-174.
    • (2006) Journal of Biochemical and Biophysical Methods , vol.49 , pp. 153-174
    • Josic, D.1    Buchacher, A.2
  • 18
    • 39749156913 scopus 로고    scopus 로고
    • Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies
    • Jungbauer A and Hahn R. Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies. Journal of Chromatography. A 2008; 1184: 62-79.
    • (2008) Journal of Chromatography. A , vol.1184 , pp. 62-79
    • Jungbauer, A.1    Hahn, R.2
  • 20
    • 0016756272 scopus 로고
    • Continuous cultures of fused cells secreting antibodies of predefined specificity
    • Kohler G and Milstein C. Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature 1975; 256: 495-497.
    • (1975) Nature , vol.256 , pp. 495-497
    • Kohler, G.1    Milstein, C.2
  • 22
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemli, U.K.1
  • 23
    • 0034528630 scopus 로고    scopus 로고
    • Anti-TNP monoclonal antibodies as reagents for enzyme immunoassay (ELISA)
    • Leo P, Ucelli P, Augusto EFP, Oliveira MS and Tamashiro WMSC. Anti-TNP monoclonal antibodies as reagents for enzyme immunoassay (ELISA). Hybridoma 2000; 19(6): 473-479.
    • (2000) Hybridoma , vol.19 , Issue.6 , pp. 473-479
    • Leo, P.1    Ucelli, P.2    Augusto, E.F.P.3    Oliveira, M.S.4    Tamashiro, W.M.S.C.5
  • 24
    • 0028219162 scopus 로고
    • A simple modification of Blum's silver stain method allows for 30 minute detection for proteins in polyacrylamide gels
    • Nesterenko MV, Michael T and Upton SJ. A simple modification of Blum's silver stain method allows for 30 minute detection for proteins in polyacrylamide gels. Journal of Biochemical and Biophysical Methods 1994; 28: 239-242.
    • (1994) Journal of Biochemical and Biophysical Methods , vol.28 , pp. 239-242
    • Nesterenko, M.V.1    Michael, T.2    Upton, S.J.3
  • 25
    • 0037040587 scopus 로고    scopus 로고
    • Multifunctional fractionation of polyclonal antibodies by immunoaffinity high-performance monolithic disk chromatography
    • Ostryanina ND, Vlasov GP and Tennikova TB. Multifunctional fractionation of polyclonal antibodies by immunoaffinity high-performance monolithic disk chromatography. Journal of Chromatography. A 2002; 949: 163-171.
    • (2002) Journal of Chromatography. A , vol.949 , pp. 163-171
    • Ostryanina, N.D.1    Vlasov, G.P.2    Tennikova, T.B.3
  • 27
    • 0016717761 scopus 로고
    • Metal chelate affinity chromatography, a new approach to protein fractionation
    • Porath J, Carlsson J, Olsson I and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 1975; 258(5536): 598-599.
    • (1975) Nature , vol.258 , Issue.5536 , pp. 598-599
    • Porath, J.1    Carlsson, J.2    Olsson, I.3    Belfrage, G.4
  • 29
    • 77952668608 scopus 로고    scopus 로고
    • Characterization of metal chelate methacrylate mnolithic disk for purification of polyclonal and monoclonal immunoglobulin G
    • Prasanna RR and Vijayalakshmi MA. Characterization of metal chelate methacrylate mnolithic disk for purification of polyclonal and monoclonal immunoglobulin G. Journal of Chromatography. A 2010; 1217: 3660-3667.
    • (2010) Journal of Chromatography. A , vol.1217 , pp. 3660-3667
    • Prasanna, R.R.1    Vijayalakshmi, M.A.2
  • 31
    • 34447527576 scopus 로고    scopus 로고
    • Affinity-based methodologies and ligands for antibody purification: advances and perspectives: review
    • Roque ACA, Silva CSO and Taipa MA. Affinity-based methodologies and ligands for antibody purification: advances and perspectives: review. Journal of Chromatography. A 2007; 1160: 44-55.
    • (2007) Journal of Chromatography. A , vol.1160 , pp. 44-55
    • Roque, A.C.A.1    Silva, C.S.O.2    Taipa, M.A.3
  • 35
    • 33847611596 scopus 로고    scopus 로고
    • Downstream processing of monoclonal antibodies- Application of platform approaches: review
    • Shukla AA, Hubbard B, Tressel T, Guhan S and Low D. Downstream processing of monoclonal antibodies- Application of platform approaches: review. Journal of Chromatography B 2010; 848: 28-39.
    • (2010) Journal of Chromatography B , vol.848 , pp. 28-39
    • Shukla, A.A.1    Hubbard, B.2    Tressel, T.3    Guhan, S.4    Low, D.5
  • 37
    • 0032758496 scopus 로고
    • Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology
    • Trebak M, Chong JM, Herlyn D and Speicher, DW. Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology. Journal of Immunological Methods 1995; 230: 59-70.
    • (1995) Journal of Immunological Methods , vol.230 , pp. 59-70
    • Trebak, M.1    Chong, J.M.2    Herlyn, D.3    Speicher, D.W.4
  • 38
    • 0347613015 scopus 로고    scopus 로고
    • Current and prospective applications of metal ion-protein binding: review
    • Ueda EKM, Gout PW and Morganti L. Current and prospective applications of metal ion-protein binding: review. Journal of Chromatography. A 2002; 988: 1-23.
    • (2002) Journal of Chromatography. A , vol.988 , pp. 1-23
    • Ueda, E.K.M.1    Gout, P.W.2    Morganti, L.3
  • 39
    • 0037049890 scopus 로고    scopus 로고
    • IMAC of human IgG: studies with IDA-immobilized copper, nickel, zinc, and cobalt ions and different buffer systems
    • Vancan S, Miranda EA and Bueno SMA. IMAC of human IgG: studies with IDA-immobilized copper, nickel, zinc, and cobalt ions and different buffer systems. Process Biochemistry 2002; 37: 573-579.
    • (2002) Process Biochemistry , vol.37 , pp. 573-579
    • Vancan, S.1    Miranda, E.A.2    Bueno, S.M.A.3
  • 40
    • 0024620848 scopus 로고
    • Pseudobiospecific ligand affinity chromatography
    • Vijayalakshmi MA. Pseudobiospecific ligand affinity chromatography. Trends in Biotechnology 1989; 7: 71-76.
    • (1989) Trends in Biotechnology , vol.7 , pp. 71-76
    • Vijayalakshmi, M.A.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.