Engineering industrial Saccharomyces cerevisiae strain with the FLO1-derivative gene isolated from the flocculating yeast SPSC01 for constitutive flocculation and fuel ethanol production

Applied Energy

Volumn 100, Issue , 2012, Pages 33-40

  He, Lei Yu ^a   Zhao, Xin Qing ^a   Bai, Feng Wu ^a  

^a DALIAN UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Fuel ethanol; Gene disruption; Integrative expression; Yeast flocculation

Indexed keywords

EXPRESSION CASSETTE; FERMENTORS; FLOCCULATING YEAST; FUEL ETHANOL; GENE DISRUPTIONS; GENE SEQUENCES; GENOMIC LIBRARIES; HIGH CELL DENSITY; HIGH CONCENTRATION; HOMOLOGOUS RECOMBINATION; INDUSTRIAL YEAST; INTEGRATIVE EXPRESSION; PCR PRODUCTS; REPEATED SEQUENCES; SACCHAROMYCES CEREVISIAE STRAINS; SELF-IMMOBILIZATION; TRANSCRIPTIONAL CONTROL; TRANSFORMANTS; YEAST CELL; YEAST STRAIN;

BIOFUEL; ETHANOL; FERMENTATION; FLOCCULATION; GENOME; IMMOBILIZATION; POLYMERASE CHAIN REACTION; RECOMBINATION; YEAST;

SACCHAROMYCES CEREVISIAE;

EID: 84867714718     PISSN: 03062619     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.apenergy.2012.03.052     Document Type: Article

References (37)

1. Verstrepen K.J., Derdelinckx G., Verachtert H., Delvaux F.R. Yeast flocculation: what brewers should know. Appl Microbiol Biotechnol 2003, 61:197-205.
2. Bai F.W., Anderson W.A., Moo-Young M. Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol Adv 2008, 26:89-105.
3. Zhao X.Q., Bai F.W. Yeast flocculation: new story in fuel ethanol production. Biotechnol Adv 2009, 27:849-856.
4. Domingues L., Vicente A.A., Lima N., Teixeira J.A. Application of yeast flocculation in biotechnological processes. Biotechnol Bioprocess Eng 2000, 5:288-305.
5. Singh R.S., Bhari R., Kaur H.P. Characteristics of yeast lectins and their role in cell-cell interactions. Biotechnol Adv 2011, 29:726-731.
6. Stratford M., Assinder S. Yeast flocculation: Flo1 and NewFlo phenotypes and receptor structure. Yeast 1991, 7:559-574.
7. Bai FW, Zhao XQ. High gravity ethanol fermentations and yeast tolerance. In: Liu ZL, editor. Microbial stress tolerance for biofuels. vol. 22. Springer. Microbiology Monographs; 2012. p. 117-35.
8. Soares E.V. Flocculation in Saccharomyces cerevisiae: a review. J Appl Microbiol 2011, 110:1-18.
9. Teunissen A.W.R.H., Steensma H.Y. Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family. Yeast 1995, 11:1001-1013.
10. Teunissen A.W.R.H., van den Berg J.A., Steensma H.Y. Physical localization of the flocculation gene FLO1 on chromosome I of Saccharomyces cerevisiae. Yeast 1993, 9:1-10.
11. Teunissen A.W.R.H., Holub E., van der Hucht J., van den Berg J.A., Steensma H.Y. Sequence of the open reading frame of the FLO1 gene from Saccharomyces cerevisiae. Yeast 1993, 9:423-427.
12. Watari J., Takata Y., Ogawa M., Sahara H., Koshino S., Onnela M.L., et al. Molecular cloning and analysis of the yeast flocculation gene FLO1. Yeast 1994, 10:211-225.
13. Bidard F., Bony M., Blondin B., Dequin S., Barre P. The Saccharomyces cerevisiae FLO1 flocculation gene encodes for a cell surface protein. Yeast 1995, 11:809-822.
14. Bony M., Thines-Sempoux D., Barre P., Blondin B. Localization and cell surface anchoring of the Saccharomyces cerevisiae flocculation protein Flo1p. J Bacteriol 1997, 179:4929-4936.
15. Govender P., Domingo J.L., Bester M.C., Pretorius I.S., Bauer F.F. Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae. Appl Environ Microbiol 2008, 74:6041-6052.
16. Guo B., Styles C.A., Feng Q., Fink G.R. A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating. Proc Natl Acad Sci USA 2000, 97:12158-12163.
17. Xu T.J., Zhao X.Q., Bai F.W. Continuous ethanol production using self-flocculating yeast in a cascade of fermentors. Enzyme Microb Tech 2005, 37:634-640.
18. Zhao XQ, Li Q, He LY, Li F, Que WW, Bai FW. Exploration of a natural reservoir of flocculating genes from various Saccharomyces cerevisiae strains and improved ethanol fermentation using stable genetically engineered flocculating yeast strains. Process Biochem, in press. doi:http://dx.doi.org/10.1016/j.procbio.2011.06.009. http://dx.doi.org/10.1016/j.procbio.2011.06.009.
19. Sambrook J., Russell D.W. Molecular cloning: a laboratory manual 2001, Cold Spring Harbor Laboratory Press, New York. 3rd ed.
20. Burke D., Dawson D., Stearns T. Methods in yeast genetics 2000, Cold Spring Harbor Laboratory Press, New York.
21. Baudin A., Ozier-Kalogeropoulos O., Denouel A., Lacroute F., Cullin C. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res 1993, 21:3329-3330.
22. Lorenz M.C., Muir R.S., Lim E., McElver J., Weber S.C., Heitman J. Gene disruption with PCR products in Saccharomyces cerevisiae. Gene 1995, 158:113-117.
23. Voth W.P., Richards J.D., Shaw J.M., Stillman D.J. Yeast vectors for integration at the HO locus. Nucleic Acids Res 2001, 29:E59.
24. Mumberg D., Müller R., Funk M. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 1995, 156:119-122.
25. Kobayashi O., Hayashi N., Kuroki R., Sone H. Region of Flo1 proteins responsible for sugar recognition. J Bacteriol 1998, 180:6503-6510.
26. Goffeau A., Barrell B.G., Bussey H., Davis R.W., Dujon B., Feldmann H., et al. Life with 6000 genes. Science 1996, 274(546):63-67.
27. Hsiao H.Y., Chiang L.C., Yang C.M., Chen L.F., Tsao G.T. Preparation and performance of immobilized yeast cells in columns containing no inert carrier. Biotechnol Bioeng 1983, 25:363-375.
28. Jones S.T., Korus R.A., Adrnassu W., Heimsch R.C. Ethanol fermentation in a continuous tower fermentor. Biotechnol Bioeng 1984, 26:742-747.
29. Kida K., Asano S.I., Yamadaki M., Iwasaki K., Yamaguchi T., Sonoda Y. Continuous high-ethanol fermentation from cane molasses by a flocculating yeast. J Ferment Bioeng 1990, 69:39-45.
30. Kida K., Morimura S., Kume K., Suruga K., Sonoda Y. Repeated-batch ethanol fermentation by a flocculating yeast Saccharomyces cerevisiae IR-2. J Ferment Bioeng 1991, 71:340-344.
31. Morimura S., Zhong Y.L., Kida K. Ethanol production by repeated-batch fermentation at high temperature in a molasses medium containing a high concentration of total Sugar by a thermotolerant flocculating yeast with improved salt-tolerance. J Ferment Bioeng 1997, 83:271-274.
32. Behera S., Mohanty R.C., Ray R.C. Ethanol production from mahula (Madhuca latifolia L.) flowers with immobilized cells of Saccharomyces cerevisiae in Luffa cylindrica L. sponge discs. Appl Energy 2011, 88:212-215.
33. Rattanapan A., Limtong S., Phisalaphong M. Ethanol production by repeated batchand continuous fermentations of blackstrap molasses using immobilized yeast cells on thin-shell silk cocoons. Appl Energy 2011, 88:4400-4404.
34. Lei J.J., Zhao X.Q., Ge X.M., Bai F.W. Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distributions. J Biotechnol 2007, 131:270-275.
35. Gibson B.R., Lawrence S.J., Leclaire J.P.R., Powell C.D., Smart K.A. Yeast responses to stresses associated with industrial brewery handling. FEMS Microbiol Rev 2007, 31:535-569.
36. Stanley D., Bandara A., Fraser S., Chambers P.J., Stanley G.A. The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae. J Appl Microbiol 2010, 109:13-24.
37. Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Intragenic tandem repeats generate functional variability. Nat Genet 2005, 37:986-990.