Heterologous expression and purification of nisa, the precursor peptide of lantibiotic nisin from lactococcus lactis

Acta Biologica Hungarica

Volumn 63, Issue 2, 2012, Pages 301-310

  Karakas Sen, Asuman ^a,b   Narbad, A ^a  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^b ULUDAG UNIVERSITY   (Turkey)

Author keywords

His tag; Nisin; Prenisin; Purification; Spliced overlap extension

Indexed keywords

BACTERIAL PROTEIN; HISTIDINE; NISA PROTEIN; NISIN; POLYHISTIDINE TAG; PROTEIN PRECURSOR; UNCLASSIFIED DRUG; BACTERIOCIN; HYBRID PROTEIN; NISIN A;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; HETEROLOGOUS EXPRESSION; LACTOCOCCUS LACTIS; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PLASMID;

BACTERIOCINS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENETIC VECTORS; LACTOCOCCUS LACTIS; NISIN; PLASMIDS; RECOMBINANT FUSION PROTEINS;

EID: 84867658895     PISSN: 02365383     EISSN: 1588256X     Source Type: Journal    
DOI: 10.1556/ABiol.63.2012.2.11     Document Type: Article

References (20)

1. Buchman, G. W., Banerjee, S., Hansen, J. N. (1988) Structure, expression and evolution of a gene encoding the precursor of nisin, a small protein antibiotic. J. Biol. Chem. 263, 16260-16266.
2. Bycroft, B. W., Chan, W. C., Roberts, G. C. K. (1991) Synthesis and characterization of pro- and prepeptides related to nisin and subtilin. In: Jung, G., Sahl, H. G. (eds) Nisin and Novel Lantibiotics. ESCOM, Leiden, pp. 204-217.
3. Casadaban, M. J., Cohen, S. N. (1980) Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138, 179-207.
4. Cotter, P. D., Hill, C., Ross, R. P. (2005) Bacteriocins: Developing innate immunity for food. Nat. Rev. Microbiol. 3, 777-788.
5. Delves-Broughton, J., Blackburn, P., Evans, R. J., Hugenholtz, J. (1996) Applications of the bacteriocin, nisin. Antonie van Leeuwenhoek 69, 193-202.
6. Dodd, H. M., Horn, N., Gasson, M. J. (1990) Analysis of the genetic determinant for the peptide antibiotic nisin. J. Gen. Microbiol. 136, 555-566.
7. Field, D., Hill, C., Cotter, P. D., Ross, R. P. (2010) The dawning of a 'Golden era' in lantibiotic bioengineering. Mol. Microbiol. 78, 1077-1087.
8. Hirel, P. H., Schmitter, M. J., Dessen, P., Fayat, G., Blanquet, S. (1989) Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc. Natl. Acad. Sci. USA 86, 8247-8251.
9. Horn, N., Martinez, M. I., Martinez, J. M., Hernandez, P. E., Gasson, M. J., Rodriguez, J. M., Dodd, H. M. (1998) Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus. Appl. Environ. Microbiol. 64, 818-823.
10. Kaletta, C., Entian, K. D. (1989) Nisin, a peptide antibiotic: cloning and sequencing of the nisA gene and post-translational processing of its peptide product. J. Bacteriol. 171, 1597-1601.
11. Karakas Sen, A., Narbad, A., Horn, N., Dodd, H. M., Parr, A. J., Colquhoun, I., Gasson, M. J. (1999) Post-translational modification of nisin. The involvement of NisB in the dehydration process. Eur. J. Biochem. 261, 524-532.
12. Kluskens, L. D., Kuipers, A., Rink, R., de Boef, E., Fekken, S., Driessen, A. J. M., Kuipers, O. P., Moll, G. N. (2005) Post-translational modification of therapeutic peptides by NisB, the dehydratase of the lantibiotic nisin. Biochemistry-US 44, 12827-12834.
13. Koponen, O., Tolonen, M., Qiao, M., Wahlström, G., Helin, J., Saris, P. E. J. (2002) NisB is required for the dehydration and NisC for the lanthionine formation in the post-translational modification of nisin. Microbiology 148, 3561-3568.
14. Kuipers, A., de Boef, E., Rink, R., Fekken, S., Kluskens, L. D., Driessen, A. J. M., Leenhouts, K., Kuipers, O. P., Moll, G. N. (2004) NisT, the transporter of the lantibiotic nisin, can transport fully modified, dehydrated, and unmodified prenisin and fusions of the leader peptide with non-lantibiotic peptides. J. Biol. Chem. 279, 22176-22182.
15. Kupke, T., Stevanovic, S., Ottenwalder, B., Metzger, J. W., Jung, G., Gotz, F. (1993) Purification and characterization of EpiA, the peptide substrate for post-translational modifications involved in epidermin biosynthesis. FEMS Microbiol. Lett. 112, 43-48.
16. Langridge, J., Langridge, P., Bergquist, P. L. (1980) Extraction of nucleic acids from agarose gels. Anal. Biochem. 103, 264-271.
17. Majer, F., Schmid, D. G., Altena, K., Bierbaum, G., Kupke, T. (2002) The flavoprotein MrsD catalyzes the oxidative decarboxylation reaction involved in formation of the peptidoglycan biosynthesis inhibitor mersacidin J. Bacteriol. 184, 1234-1243.
18. Nilsson, B. L., Soellner, M. B., Raines, R. T. (2005) Chemical synthesis of proteins. Annu. Rev. Biophys. Biomol. Struct. 34, 91-118.
19. Van der Meer, J. R., Polman, J., Beerthuyzen, M. M., Siezen, R. J., Kuipers, O. P., de Vos, W. M. (1993) Characterization of the Lactococcus lactis nisin A operon genes nisP, encoding a subtilisinlike serine protease involved in precursor processing, and nisR, encoding a regulatory protein involved in nisin biosynthesis. J. Bacteriol. 175, 2578-2588.
20. Xie, L., Miller, L. M., Chatterjee, C., Averin, O., Kelleher, N. L., van der Donk, W. A. (2004) Lacticia 481: In vitro reconstruction of lantiobiotic segWWWWbase activity. Science 303, 679-681.