The flavoprotein MrsD catalyzes the oxidative decarboxylation reaction involved in formation of the peptidoglycan biosynthesis inhibitor mersacidin

Journal of Bacteriology

Volumn 184, Issue 5, 2002, Pages 1234-1243

  Majer, Florian ^a   Schmid, Dietmar G ^a   Altena, Karsten ^a   Bierbaum, Gabriele ^a   Kupke, Thomas ^a  

^a Lehrstuhl fur Mikrobielle Genetik   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOTHIOL; ASPARAGINE; BACTERIAL DNA; CARBOXYLYASE; COENZYME A; CYSTEINE; FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; FLAVOPROTEIN; HISTIDINE; HOMOOLIGOMERIC FLAVIN CONTAINING CYSTEINE DECARBOXYLASE; LANTIBIOTIC; MALTOSE BINDING PROTEIN; MERSACIDIN; PEPTIDOGLYCAN; PRECURSOR LIPID II; PROTEIN DFP; PROTEIN EPID; PROTEIN MRSA; PROTEIN MRSD; PROTEIN PRECURSOR; SULFIDE; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS; BACTERIAL METABOLISM; BIOSYNTHESIS; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CHEMICAL BOND; CHEMICAL REACTION; DECARBOXYLATION; DNA SEQUENCE; DRUG STRUCTURE; DRUG SYNTHESIS; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STRUCTURE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN PROCESSING; REACTION ANALYSIS; SALT TOLERANCE; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

BACTERIA (MICROORGANISMS); EUKARYOTA; HOMO; POSIBACTERIA;

EID: 0036178887     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.184.5.1234-1243.2002     Document Type: Article

References (38)

1. The X-ray structure of the FMN-binding protein AtHa13 provides the structural basis for the activity of a regulatory subunit involved in signal transduction
2. Elucidation of the structure of epidermin, a ribosomally synthesized, tetracyclic heterodetic polypeptide antibiotic
3. Biosynthesis of the lantibiotic mersacidin: Organization of a type B lantibiotic gene cluster
4. Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate
5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
6. The lantibiotic mersacidin inhibits peptidoglycan synthesis by targeting lipid II
7. The lantibiotic mersacidin inhibits peptidoglycan biosynthesis at the level of transglycosylation
8. Mersacidin, a new antibiotic from Bacillus: In vitro and in vivo antibacterial activity
9. Mersacidin, a new antibiotic from Bacillus: Fermentation, isolation, purification and chemical characterization
10. Directed mutagenesis using the polymerase chain reaction
11. Arabidopsis thaliana AtHa13: A flavoprotein related to salt and osmotic tolerance and plant growth
12. Gallidermin: A new lanthionine-containing polypeptide antibiotic
13. 13C-labeled substrate
14. Paper chromatography of flavins and flavin nucleotides
15. NMR-spectroscopic investigation on mersacidin
16. Molecular characterization of the 4′-phosphopantothenoyl-cysteine decarboxylase domain of bacterial Dfp flavoproteins
17. a value of the enethiol side chain is lower than that of the thiol side chain of peptides
18. In vivo reaction of affinity-tag-labelled epidermin precursor peptide with flavoenzyme EpiD
19. Arabidopsis thaliana flavoprotein AtHAL3a catalyzes the decarboxylation of 4′-phosphopantothenoylcysteine to 4′-phosphopantetheine, a key step in coenzyme A biosynthesis
20. Mass spectroscopic analysis of a novel enzymatic reaction: Oxidative decarboxylation of the lantibiotic precursor peptide EpiA catalyzed by the flavoprotein EpiD
21. Oxidative decarboxylation of peptides catalyzed by flavoprotein EpiD: Determination of substrate specificity using peptide libraries and neutral loss mass spectrometry
22. Purificalion and characterization of EpiA, the peptide substrate for posttranslational modifications involved in epidermin biosynthesis
23. Purification and characterization of EpiD, a flavoprotein involved in the biosynthesis of the lantibiotic epidermin
24. Molecular characterization of lantibiotic-synthesizing enzyme EpiD reveals a function for bacterial Dfp proteins in coenzyme A biosynthesis
25. Characterization of rat LANCL1, a novel member of the lanthionine synthetase C-like protein family, highly expressed in testis and brain
26. Nucleotide sequence of the lantibiotic Pep5 biosynthetic gene cluster and functional analysis of PepP and PepC: Evidence for a role of PepC in thioether formation
27. Structure of cypemycin, a new peptide antibiotic
28. Purification and structure of mutacin B-Ny266: A new lantibiotic produced by Streptococcus mutans
29. Purification and biochemical characterization of mutacin I from the group I strain of Streptococcus mutans, CH43, and genetic analysis of mutacin I biosynthesis genes
30. Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes
31. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
32. Ab initio structure determination of the lantibiotic mersacidin
33. Prepeptide sequence of epidermin, a ribosomally synthesized antibiotic with four sulphide-rings
34. Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics
35. dfp gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein
36. Mechanistic studies on phosphopantothenoylcysteine decarboxylase
37. Phosphopantothenoylcysteine synthetase from Escherichia coli: Identification and characterization of the last unidentified coenzyme A biosynthetic enzyme in bacteria
38. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications