Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts

Journal of Clinical Virology

Volumn 51, Issue 3, 2011, Pages 179-185

  Jansen, Rogier R ^a   Schinkel, Janke ^a   Koekkoek, Sylvie ^a   Pajkrt, Dasja ^a   Beld, Marcel ^a,b   Jong, Menno D de ^a   Molenkamp, Richard ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b ROYAL TROPICAL INSTITUTE   (Netherlands)

Author keywords

Multiplex; PCR; Realtime; Respiratory; Virus

Indexed keywords

ADENOVIRUS; ADOLESCENT; ADULT; ARTICLE; CHILD; CONTROLLED STUDY; CORONAVIRUS; ENTEROVIRUS; EQUINE VIRAL ARTERITIS VIRUS; EVALUATION; FEMALE; HUMAN; INFLUENZA VIRUS A; INFLUENZA VIRUS B; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; METAPNEUMOVIRUS; MULTIPLEX POLYMERASE CHAIN REACTION; MULTIPLEX REAL TIME POLYMERASE CHAIN REACTION; NOSE SMEAR; PARAINFLUENZA VIRUS 1; PARAINFLUENZA VIRUS 2; PARAINFLUENZA VIRUS 3; PARAINFLUENZA VIRUS 4; PARECHOVIRUS; PRESCHOOL CHILD; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY VIRUS; RHINOVIRUS; SCHOOL CHILD; SENSITIVITY AND SPECIFICITY; THROAT CULTURE; VALIDATION STUDY;

CHILD, PRESCHOOL; FEMALE; HUMANS; INFANT; MALE; MOLECULAR DIAGNOSTIC TECHNIQUES; NASOPHARYNX; PLASMIDS; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS DISEASES; VIRUSES;

EID: 79959244400     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2011.04.010     Document Type: Article

References (15)

1. Brittain-Long R., Nord S., Olofsson S., Westin J., Anderson L.M., Lindh M. Multiplex real-time PCR for detection of respiratory tract infections. J Clin Virol 2008, 41(1):53-56.
2. Mahony J.B., Blackhouse G., Babwah J., Smieja M., Buracond S., Chong S., et al. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol 2009, 47(9):2812-2817.
3. Wang W., Ren P., Sheng J., Mardy S., Yan H., Zhang J., et al. Simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-PCR assays. J Virol Methods 2009, 162(1-2):40-45.
4. Freymuth F., Vabret A., Cuvillon-Nimal D., Simon S., Dina J., Legrand L., et al. Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. J Med Virol 2006, 78(11):1498-1504.
5. van Elden L.J., van Kraaij M.G., Nijhuis M., Hendriksen K.A., Dekker A.W., Rozenberg-Arska M., et al. Polymerase chain reaction is more sensitive than viral culture and antigen testing for the detection of respiratory viruses in adults with hematological cancer and pneumonia. Clin Infect Dis 2002, 34(2):177-183.
6. van de Pol A.C., van Loon A.M., Wolfs T.F., Jansen N.J., Nijhuis M., Breteler E.K., et al. Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms. J Clin Microbiol 2007, 45(7):2260-2262.
7. Puppe W., Weigl J.A., Aron G., Grondahl B., Schmitt H.J., Niesters H.G., et al. Evaluation of a multiplex reverse transcriptase PCR ELISA for the detection of nine respiratory tract pathogens. J Clin Virol 2004, 30(2):165-174.
8. Gunson R.N., Collins T.C., Carman W.F. Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions. J Clin Virol 2005, 33(4):341-344.
9. Beld M., Minnaar R., Weel J., Sol C., Damen M., van der Avoort H., et al. Highly sensitive assay for detection of enterovirus in clinical specimens by reverse transcription-PCR with an armored RNA internal control. J Clin Microbiol 2004, 42(7):3059-3064.
10. Scheltinga S.A., Templeton K.E., Beersma M.F., Claas E.C. Diagnosis of human metapneumovirus and rhinovirus in patients with respiratory tract infections by an internally controlled multiplex real-time RNA PCR. J Clin Virol 2005, 33(4):306-311.
11. Molenkamp R., van der H.A., Schinkel J., Beld M. Simultaneous detection of five different DNA targets by real-time Taqman PCR using the Roche LightCycler480: application in viral molecular diagnostics. J Virol Methods 2007, 141:205-211.
12. Wittner C.T., Kusukawa N. Real-Time PCR. Molecular microbiology: diagnostic principles and practice 2004, ASM Press, Washington, DC, p. 71-4. D.H. Persing (Ed.).
13. Lu X., Holloway B., Dare R.K., Kuypers J., Yagi S., Williams J.V., et al. Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses. J Clin Microbiol 2008, 46(2):533-539.
14. Tapparel C., Junier T., Gerlach D., Van-Belle S., Turin L., Cordey S., et al. New respiratory enterovirus and recombinant rhinoviruses among circulating picornaviruses. Emerg Infect Dis 2009, 15(5):719-726.
15. Tapparel C., Cordey S., Van B.S., Turin L., Lee W.M., Regamey N., et al. New molecular detection tools adapted to emerging rhinoviruses and enteroviruses. J Clin Microbiol 2009, 47(6):1742-1749.