Enhanced cellulase production of Penicillium decumbens by knocking out CreB encoding a deubiquitination enzyme

Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

Volumn 28, Issue 8, 2012, Pages 959-972

  Zhou, Guangqi ^a   Lü, Jing ^a   Li, Zhonghai ^b   Li, Jingjing ^a   Wang, Mingyu ^b   Qu, Yinbo ^b   Xiao, Lin ^c,d   Qin, Shulin ^c,d   Zhao, Haitao ^c,d   Xia, Ruirui ^c,d   Fang, Xu ^b  

^a DALIAN POLYTECHNIC UNIVERSITY   (China)

^b SHANDONG UNIVERSITY   (China)

^c Longlive Bio Technology Co Ltd   (China)

^d Shandong Key Laboratory of Straw and Stover Biorefinement Technologies   (China)

Author keywords

Cellulase; CREB; Deubiquitination; Penicillium decumbens

Indexed keywords

CELLULASE; LIGNIN; LIGNOCELLULOSE; MUTANT PROTEIN; PROTEINASE; UBIQUITINATED PROTEIN;

ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; GENE INACTIVATION; GENETIC RECOMBINATION; GENETICS; METABOLISM; PENICILLIUM; UBIQUITINATION;

CELLULASE; ENDOPEPTIDASES; GENE KNOCKOUT TECHNIQUES; LIGNIN; MUTANT PROTEINS; PENICILLIUM; RECOMBINATION, GENETIC; UBIQUITINATED PROTEINS; UBIQUITINATION;

EID: 84867606420     PISSN: 10003061     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Gao L, Wang FH, Gao F, et al. Purification and characterization of a novel cellobiohydrolase (PdCel6A) from Penicillium decumbens JU-A10 for bioethanol production. Bioresour Technol, 2011, 102(17): 8339-8342.
2. Chen S, Xing XH, Huang JJ, et al. Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: Improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase. Enzyme Microb Technol, 2011, 48(1): 100-105.
3. Wei XM, Qin YQ, Qu YB. Molecular cloning and characterization of two major endoglucanases from Penicillium decumbens. J Microbiol Biotechnol, 2010, 20(2): 265-270.
4. Portnoy T, Margeot A, Linke R, et al. The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilation. BMC Genomics, 2011, 12(1): 269.
5. Lockington RA, Kelly JM. Carbon catabolite repression in Aspergillus nidulans involves deubiquitination. Mol Microbiol, 2001, 40(6): 1311-1321.
6. Lockington RA, Kelly JM. The WD40-repeat protein CreC interacts with and stabilizes the deubiquitinating enzyme CreB in vivo in Aspergillus nidulans. Mol Microbiol, 2002, 43(5): 1173-1182.
7. Boase NA, Kelly JM. A role for creD, a carbon catabolite repression gene from Aspergillus nidulans, in ubiquitination. Mol Microbiol, 2004, 53(3): 929-940.
8. Nehlin JQ, Carberg M, Ronne H. Control of yeast GAL genes by Mig1p repressor: a transcriptional cascade in the glucose response. EMBO J, 1991, 10: 3373-3377.
9. Arst HN, Tollervey D, Dowzer CEA, et al. An inversion truncating the creA gene of Aspergillus nidulans results in carbon catabolite derepression. Mol Microbiol, 1990, 4(5): 851-854.
10. Rogers S, Wells R, Rechsteiner M. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science, 1986, 234(4774): 364-368.
11. Denton JA, Kelly JM. Disruption of Trichoderma reesei cre2, encoding an ubiquitin C-terminal hydrolase, results in increased cellulase activity. BMC Biotechnol, 2011, 11(1): 103.
12. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press, 2001.
13. Li ZH. Development of a highly efficient gene targeting system allowing rapid genetic manipulations and functional analysis of the transcriptional regulator CreA in Penicillium decumbens[D]. Jinan: Shandong University, 2010.
14. Sun XY. Studies on the Synthesis Regulation of lignoeellulose-degrading enzymes in Penicillium decumbens[D]. Jinan: Shandong University, 2007.
15. Yu JH, Hamari Z, Han KH, et al. Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet Biol, 2004, 41(11): 973-981.
16. Kubodera T, Yamashita N, Nishimura A. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci Biotechnol Biochem, 2000, 64(7): 1416-1421.
17. Honda S, Selker EU. Tools for fungal proteomics: multifunctional Neurospora vectors for gene replacement, protein expression and protein purification. Genetics, 2009, 182(1): 11-23.
18. Ninomiya Y, Suzuki K, Ishii C, et al. Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proc Natl Acad Sci USA, 2004, 101(33): 12248-12253.
19. Lara-Rojas F, Sánchez O, Kawasaki L, et al. Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions. Mol Microbiol, 2011, 80(2): 436-454.
20. Carvalho NDSP, Arentshorst M, Kooistra R, et al. Effects of a defective ERAD pathway on growth and heterologous protein production in Aspergillus niger. Appl Microbiol Biotechnol, 2011, 89(2): 357-373.
21. Li ZH, Du CM, Zhong YH, et al. Development of a highly efficient gene targeting system allowing rapid genetic manipulations in Penicillium decumbens. Appl Microbiol Biotechnol, 2010, 87(3): 1065-1076.
22. van Peij NNME, Brinkmann J, Vršanská M, et al. β-Xylosidase activity, encoded by xlnD, is essential for complete hydrolysis of xylan by Aspergillus niger but not for induction of the xylanolytic enzyme spectrum. Eur J Biochem, 1997, 245(1): 164-173.
23. van Peij NNME, Gielkens MMC, De Vries RP, et al. The transcriptional activator XlnR regulates both xylanolytic and endoglucanase gene expression in Aspergillus niger. Appl Environ Microbiol, 1998, 64(10): 3615-3619.
24. Sun J, Glass NL. Identification of the CRE-1 cellulolytic regulon in Neurospora crassa. PLoS One, 2011, 6(9): e25654.
25. Portnoy T, Margeot A, Seidl-Seiboth V, et al. Differential regulation of the cellulase transcription factors XYR1, ACE2, and ACE1 in Trichoderma reesei strains producing high and low levels of cellulase. Eukaryot Cell, 2011, 10(2): 262-271.
26. Andersen MR, Nielsen ML, Nielsen J. Metabolic model integration of the bibliome, genome, metabolome and reactome of Aspergillus niger. Mol Syst Biol, 2008, 4(1): 178.
27. Furukawa T, Shida Y, Kitagami N, et al. Identification of specific binding sites for XYR1, a transcriptional activator of cellulolytic and xylanolytic genes in Trichoderma reesei. Fungal Genet Biol, 2009, 46(8): 564-574.
28. Tamayo EN, Villanueva A, Hasper AA, et al. CreA mediates repression of the regulatory gene xlnR which controls the production of xylanolytic enzymes in Aspergillus nidulans. Fungal Genet Biol, 2008, 45(6): 984-993.
29. Hasper AA, Trindade LM, van der Veen D, et al. Functional analysis of the transcriptional activator XlnR from Aspergillus niger. Microbiology, 2004, 150(5): 1367-1375.
30. 2 transcription factor involved in regulation of activity of the cellulase promoter cbh1 of Trichoderma reesei. J Biol Chem, 2000, 275(8): 5817-5825.