Purification and characterization of a novel cellobiohydrolase (PdCel6A) from Penicillium decumbens JU-A10 for bioethanol production

Bioresource Technology

Volumn 102, Issue 17, 2011, Pages 8339-8342

  Gao, Le ^a   Wang, Fenghui ^a   Gao, Feng ^a   Wang, Lushan ^a   Zhao, Jian ^a   Qu, Yinbo ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Cel6A; Cellobiohydrolase; Endo action; Penicillium decumbens

Indexed keywords

AMINO ACID SEQUENCE; ASPERGILLUS FUMIGATUS; BIO-ETHANOL PRODUCTION; CEL6A; CELLOBIOHYDROLASES; CELLULOSE DEGRADATION; ENDO-ACTION; ETHANOL CONCENTRATIONS; LOW DOSE; PENICILLIUM DECUMBENS; SEQUENCE IDENTITY; SIMULTANEOUS SACCHARIFICATION AND FERMENTATION; SPECIFIC ACTIVITY;

AMINO ACIDS; BIOETHANOL; ETHANOL; PURIFICATION; SACCHARIFICATION; SUBSTRATES;

ENZYME ACTIVITY;

CELLOBIOSE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE I; CELLULOSE 1,4 BETA CELLOBIOSIDASE II; FUNGAL ENZYME; PDCEL6A ENZYME; UNCLASSIFIED DRUG;

AMINO ACID; BIOFUEL; CELLULOSE; CONCENTRATION (COMPOSITION); CROP RESIDUE; DEGRADATION; ENZYME; ENZYME ACTIVITY; ETHANOL; FERMENTATION; FUNGUS; INDUSTRIAL PRODUCTION; PHENOLIC COMPOUND; PURIFICATION; SUBSTRATE;

ALCOHOL PRODUCTION; ARTICLE; ASPERGILLUS FUMIGATUS; BACTERIAL STRAIN; CONTROLLED STUDY; DEGRADATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; NONHUMAN; PENICILLIUM; PENICILLIUM DECUMBENS; PH; PRIORITY JOURNAL; SACCHARIFICATION; TEMPERATURE;

AMINO ACID SEQUENCE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; ETHANOL; MOLECULAR SEQUENCE DATA; PENICILLIUM; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ASPERGILLUS FUMIGATUS; PENICILLIUM DECUMBENS;

EID: 79960846488     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2011.06.033     Document Type: Article

References (14)

1. Boisset C., Fraschini C., Schülein M., Henrissat B., Chanzy H. Imaging the enzymatic digestion of bacterial cellulose ribbons reveals the endo character of the cellobiohydrolase Cel6A from Humicola insolens and its mode of synergy with cellobiohydrolase Cel7A. Appl. Environ. Microbiol. 2000, 66:1444-1452.
2. Cheng Y.F., Song X., Qin Y.Q., Qu Y.B. Genome shuffling improves production of cellulase by Penicillium decumbens JU-A10. J. Appl. Microbiol. 2009, 107:1837-1846.
3. Deshpande M.V., Eriksson K.E., Pettersson L.G. An assay for selective determination of exo-1,4-β-glucanases in a mixture of cellulolytic enzymes. Anal. Biochem. 1984, 138:481-487.
4. Gusakova A.V., Sinitsyna A.P., Salanovicha T.N., Bukhtojarova F.E., Markova A.V., Ustinova B.B., Zeijlb C.V., Puntb P., Burlingame R. Purification, cloning and characterisation of two forms of thermostable and highly active cellobiohydrolase I (Cel7A) produced by the industrial strain of Chrysosporium lucknowense. Enzyme Microb. Technol. 2005, 36:57-69.
5. Henriksson K., Teleman A., Suortti T., Reinikainen T., Jaskari J., Teleman O., Poutanen K. Hydrolysis of barley (1-3),(1-4)-β-d-glucan by a cellobiohydrolase II preparation from Trichoderma reesei. Carbohydr. Polym. 1995, 26:109-119.
6. Han Y.J., Chen H.Z. Synergism between corn stover protein and cellulase. Enzyme Microb. Technol. 2007, 41:638-645.
7. Lahjouji K., Storms R., Xiao Z.Z., Joung K.B., Zheng Y., Powlowski J., Tsang A., Varin L. Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor. Appl. Microbiol. Biotechnol. 2007, 75:337-346.
8. Lee K.M., Joo A.R., Jeya M., Lee K.M., Moon H.J., Lee J.K. Production and Characterization of Cellobiohydrolase from a Novel Strain of Penicillium purpurogenum KJS506. Appl. Biochem. Biotechnol. 2011, 163:25-39.
9. Limam F., Chaabouni S.E., Ghrir R., Marzouki N. Two cellobiohydrolases of Penicillium occitanis mutant Pol 6: Purification and properties. Enzyme Microb. Technol. 1995, 17(7):340-346.
10. Liu K., Lin X.H., Yue J., Li X.Z., Fang X., Zhu M.T., Lin J.Q., Qu Y.B., Xiao L. High concentration ethanol production from corncob residues by fed-batch strategy. Bioresour. Technol. 2010, 101:4952-4958.
11. Okada H., Sekiya T., Yokoyama K., Tohda H., Kumagai H., Morikawa Y. Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizosaccharomyces pombe and characterization of its products. Appl. Microbiol. Biotechnol. 1998, 49:301-308.
12. Sun X.Y., Liu Z.Y., Zheng K., Song X., Qu Y.B. The composition of basal and induced cellulase systems in Penicillium decumbens under induction or repression conditions. Enzyme Microb. Technol. 2008, 42:560-567.
13. Tuohy M.G., WalshD J., Murray P.G., Claeyssens M., Cuffe M.M., SavageA V., Coughlan M.P. Kinetic parameters and mode of action of the cellobiohydrolases produced by Talaromyces emersonii. Biochim. Biophys. Acta 2002, 1596:366-380.
14. Yagüe E., Chow C.M., Challen M.P., Thurston C.F. Correlation of exons with functional domains and folding regions in a cellulase from Agaricus bisporus. Curr. Genet. 1996, 30:56-61.