Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening

Protein Expression and Purification

Volumn 86, Issue 2, 2012, Pages 120-126

  Ma, Yi ^a,b   Ghoshdastider, Umesh ^b,c   Wang, Jufang ^a   Ye, Wei ^a   Dötsch, Volker ^b   Filipek, Slawomir ^d   Bernhard, Frank ^b   Wang, Xiaoning ^a,e  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b GOETHE UNIVERSITY   (Germany)

^c INTERNATIONAL INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Poland)

^d UNIVERSITY OF WARSAW   (Poland)

^e CHINESE PLA GENERAL HOSPITAL   (China)

Author keywords

Cell free expression; GNA1; Inhibitor screening; Simulated annealing

Indexed keywords

GLUCOSAMINE PHOSPHATE ACETYLTRANSFERASE; GLUCOSE 6 PHOSPHATE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; BIOSYNTHESIS; BIOTECHNOLOGY; CELL FREE SYSTEM; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; GENETICS; HUMAN; KINETICS; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; BINDING SITES; BIOTECHNOLOGY; CELL-FREE SYSTEM; GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE; GLUCOSE-6-PHOSPHATE; GREEN FLUORESCENT PROTEINS; HUMANS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

EID: 84867595688     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.09.011     Document Type: Article

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