Glucose-6-phosphate as a probe for the glucosamine-6-phosphate N-acetyltransferase Michaelis complex

FEBS Letters

Volumn 581, Issue 29, 2007, Pages 5597-5600

  Hurtado Guerrero, Ramon ^a   Raimi, Olawale ^a   Shepherd, Sharon ^a   van Aalten, Daan M F ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Acetyltransferase; Crystal structure; Inhibitor; Kinetics; Michaelis complex; UDP GlcNAc

Indexed keywords

ACETYL COENZYME A; ANTIFUNGAL AGENT; GLUCOSAMINE PHOSPHATE ACETYLTRANSFERASE; GLUCOSE 6 PHOSPHATE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

ACETYLATION; ARTICLE; BIOSYNTHESIS; CRYSTAL STRUCTURE; DRUG TARGETING; MICHAELIS MENTEN KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT;

ASPERGILLUS FUMIGATUS; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; GLUCOSAMINE; GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE; GLUCOSE-6-PHOSPHATE; KINETICS; MUTAGENESIS; SUBSTRATE SPECIFICITY;

DIPTERA;

EID: 36549056086     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.10.065     Document Type: Article

References (18)

1. Katz D., and Rosenberger R.F. A mutation in aspergillus nidulans producing hyphal walls which lack chitin. Biochim. Biophys. Acta 208 (1970) 452-460
2. Takeda J., and Kinoshita T. GPI-anchor biosynthesis. Trends Biochem. Sci. 20 (1995) 367-371
3. Selitrennikoff C.P., and Sonneborn D.R. The last two pathway-specific enzyme activities of hexosamine biosynthesis are present in blastocladiella emersonii zoospores prior to germination. Biochim. Biophys. Acta 451 (1976) 408-416
4. Hofmann M., Boles E., and Zimmermann F.K. Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase. Eur. J. Biochem. 221 (1994) 741-747
5. Etchebehere L.C., Simon M.N., Campanhã R.B., Zapella P.D., Véron M., and Maia J.C. Developmental regulation of hexosamine biosynthesis by protein phosphatases 2a and 2c in blastocladiella emersonii. J. Bacteriol. 175 (1993) 5022-5027
6. Vetting M.W., de Carvalho L.P.S., Yu M., Hegde S.S., Magnet S., Roderick S.L., and Blanchard J.S. Structure and functions of the gnat superfamily of acetyltransferases. Arch. Biochem. Biophys. 433 (2005) 212-226
7. Peneff C., Mengin-Lecreulx D., and Bourne Y. The crystal structures of apo and complexed saccharomyces cerevisiae gna1 shed light on the catalytic mechanism of an amino-sugar n-acetyltransferase. J. Biol. Chem. 276 (2001) 16328-16334
8. Hurtado, R., Plotnikov, A.N. and van Aalten, D.M.F. (in preparation) Structural and kinetic analysis of human and Aspergillus fumigatus glucosamine n-acetyl transferase reveals exploitable active site differences.
9. Riddles P., Blakeley R., and Zerner B. Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid) - a reexamination. Anal. Biochem. 97 (1979) 75-81
10. Gehring A.M., Lees W.J., Mindiola D.J., Walsh C.T., and Brown E.D. Acetyltransfer precedes uridylyltransfer in the formation of udp-n-acetylglucosamine in separable active sites of the bifunctional glmu protein of Escherichia coli. Biochemistry 35 (1996) 579-585
11. Leatherbarrow R.J. GraFit Version 5 (2001), Erithacus Software Ltd., Horley, UK
12. Murshudov G.N., Vagin A.A., and Dodson E.J. Refinement of macromolecular structures by the maximum-likelihood method. Acta Cryst. D 53 (1997) 240-255
13. Emsley P., and Cowtan K. Coot: model-building tools for molecular graphics. Acta Cryst. D 60 (2004) 2126-2132
14. Schuettelkopf A.W., and van Aalten D.M.F. Prodrg: a tool for high-throughput crystallography of protein-ligand complexes. Acta Cryst. D 60 (2004) 1355-1363
15. Vriend G. WHAT IF: a molecular modeling and drug design program. J. Mol. Graph. 8 (1990) 52-56
16. DeLano, W.L. (2004). Use of pymol as a communications tool for molecular science. Abstr. Pap. Am. Chem. Soc. 228, 030-CHED.
17. Buergi H.B., Dunitz J.D., Lehn J.M., and Wipff G. Stereochemistry of reaction. paths at carbonyl centers. Tetrahedron 30 (1974) 1563-1572
18. Mio T., Yamada-Okabe T., Arisawa M., and Yamada-Okabe H. Saccharomyces cerevisiae gna1, an essential gene encoding a novel acetyltransferase involved in udp-n-acetylglucosamine synthesis. J. Biol. Chem. 274 (1999) 424-429