Hypolipidemic and hypocholestrolemic effect of medicinal plant combination in the diet of rats: Black cumin seed (Nigella sativa) and turmeric (Curcumin)

Journal of Animal and Veterinary Advances

Volumn 11, Issue 12, 2012, Pages 2013-2019

  Al Nazawi, M H ^a   El Bahr, S M ^a  

^a KING FAISAL UNIVERSITY   (Saudi Arabia)

Author keywords

Biochemical parameters; Blood; Lipid profile; Medicinal plants; Rats; Serum

Indexed keywords

EID: 84867383026     PISSN: 16805593     EISSN: 1993601X     Source Type: Journal    
DOI: 10.3923/javaa.2012.2013.2019     Document Type: Article

References (60)

1. AOAC, 1995. Official Methods of Analysis. 16th Edn., Association of Official Analytical Chemists, Washington, DC, USA.
2. AbdEl-Aal, E.S.M. andR.S. Attia, 1993. Characterization of black cumin (Nigella sativa): Chemical composition and lipid. Alex. Sci. Exch., 14: 467-467.
3. Abu-Al-Basal, M.A., 2011. Influence of Nigella sativa fixed oil on some blood parameters and histopathology of skin in staphylococcal-infected BALB/c mice. Pak. J. Biol. Sci., 14: 1038-1046.
4. Agarwal, P., M.D. Kharya and R. Shrivastava, 1979. Antimicrobial and anthelmintic activities of the essential oil of Nigella sativa L. Ind. J. Exp. Biol., 17: 1264-1275.
5. Aggarwal, B.B., A. Kumar, M.S. Aggarwal and S. Shishodia, 2005. Curcumin Derived From Turmeric (Curcuma longa): A Spice for all Seasons. In: Phytopharmaceuticals in Cancer Chemoprevention, Preuss, H.G. (Eds). CRC Press Inc., Boca Raton, USA, pp: 349-387.
6. Akhtar, M.S., Z. Nasir and A.R., Abid, 2003. Effect of feeding powdered Nigella sativa L. seeds on poultry egg production and their suitability for human consumption. Veterinarski Arch., 73: 181-190.
7. Alsaif, M.A., 2008. Effect of Nigella sativa oil on metabolic responses to prolonged systemic injury in rats. J. Boil. Sci., 8: 974-983.
8. Badari, O.A., A.B. Abdel-Naim, M.H. Abdel-Wahab and F.M. Hamada, 2000. The influence of thymoquinon on doxorubicin-induced hyperlipidemic nephropathy in rats. Toxicology, 143: 219-226.
9. Bamosa, A.O., B.A. Ali and S.A. Sowayan, 1997. Effect of oral ingestion of Nigella sativa seeds on some blood parameters. Saudi Pharm. J., 5: 126-129.
10. Basavaraj, M., V. Nagabhushana, N. Prakash, M.M. Appannavar, P. Wagmare and S. Malhkarjunappa, 2011. Effect of dietary supplementation of Curcuma longa on the biochemical profile and meat characteristics of broiler rabbits under summer stress. Vet. World, 4: 15-18.
11. Bashandy, S.A.E., 1996. Effect of Nigella sativa oil on liver and kidney functions of adult and senile rats. Egypt. J. Pharm. Sci., 37: 313-327.
12. Bauer, ID., 1982. Clinical Laboratory Methods. 9th Edn., The C.V. Company II 1830, Westlme Industrial, Missouri.
13. Buriro, M.A. and M. Tayyab, 2007. Effect of Nigella sativa on lipid profile in albino rats. Gomal J. Med. Sci., 5: 28-31.
14. Chaudary, S.A., 1996. Serum lipid profile in albino rats fed on atherogenic supplemented palm oil diet and Nigella sativa. Ph.D. Thesis, Department of Pathology, Postgraduate Medical Institute, Lahore, Pakistan.
15. Chou Y.H., 1975. Experimental Design and the Analysis of Variance, Statistical Analysis-I. Holt, Reinhart and Winston Publication, New York, pp: 340-352.
16. Dehkordi, F.R. and A.F. Kamkhah, 2008. Antihypertensive effect of Nigella sativa seed extract in patients with mild hypertension. Fundam. Clin. Pharmacol., 22: 447-452.
17. Doumas, B.T., D.D. Bayson, RJ. Carter, T. Peters and R. Schaffer, 1981. Estimation of total serum protein. Clin. Chem., 27: 1642-1643.
18. Ekanem, J.T. and O.K. Yusuf, 2008. Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. ^rwcei-infected rats. Afr. J. Biomed. Res., 11:79-85.
19. El-Bagir, N.M., I.T.O. Farah, A. Alhaidary, H.E. Mohamed and A.C. Beynen, 2010. Clinical laboratory serum values in rabbits fed diets containing black cumin seed. J. Arum. Vet. Adv., 9: 2532-2536.
20. El-Bahr, S.M. and T.T. Saad, 2008. Effect of black cumin seeds (Nigella sativa) and/or Turmeric (Curcumin) on hematological, biochemical and immunological parameters of Mugil cephalus fish vaccinated with Aeromonus hydrophila bacterm. Proceedings of the 13th Scientific Congress, November 25-28, 2008, Faculty of Veterinary Medicine, As suit University, pp: 365-388.
21. El-Bahr, S.M., 2007. Effect of black cumin seeds (Nigella sativa) on the profile of serum lipids, lipoproteins and fatty acids in pekin ducklings. Int. J. Applied Chem., 3: 221-230.
22. El-Bahr, S.M., M.A. Korshom, A.E.A. Mandour, A.A. El-Bessomy and M.A. Lebdah, 2007. The protective effect of Turmeric on iron overload in albino rats. Egypt. J. Biochem. Mol. Biol., 25: 94-113.
23. El-Beshbishy, H.A., AN.B. Singab, J. Sinkkonen and L. Pihlaja, 2006. Hypohpidemic and antioxidant effects of Morus alba L. (Egyptian mulberry) root bark fractions supplementations in cholesterol fed rats. Life Sci., 78: 2724-2733.
24. El-Dakhakhny, M., N.I. Mady and M.A. Halim, 2000. Nigella sativa L. oil protects against induced hepatotoxicity and improves serum lipid profile in rats. Arzneimittelforschung, 50: 832-836.
25. Emadi, M., H. Kermanshahi and E. Maroufyan, 2007. Effect of varying levels of turmeric rhizome powder on some blood parameters of broiler chickens fed corn soybean meal based diets. Int. J. Poult. Sci., 6: 345-348.
26. Genest, J.J., M. Marcil, M. Denis and L. Yu, 1999. High density hpoprotems in health and in disease. J. Investig. Med, 47: 31-42.
27. Gilam, A.H., Q.Jabeen andM.A.U. Khan, 2004. A review of medicinal uses and pharmacological activities of mgella sativa. Pak. J. Biol. Sci., 7: 441-451.
28. Gottfried S.P. and B. Rosenberg, 1973. Improved manual spectrophotometric procedure for determination of serum triglycerides. Clin. Chem., 19: 1077-1078.
29. Hasan, M.M., S.P. Chowdhury, Shahidul Alam, B. Hossain and M.S. Alam, 2005. Antifungal effects of plant extracts on seed-borne fungi of wheat seed regarding seed germination, Seedling health and vigour index. Pak. J. Biol. Sci., 8: 1284-1289.
30. Hassamn, N.I. and F.M. Hassan, 1996. A preliminary study on the effect of Mgella sativa L. seeds on hypoglycemia. Vet. Med. J. Giza, 44: 699-708.
31. Hedaya, S.A., 1995. Effect of Mgella sativa (black seeds) extract on some haematological and biochemical parameters in rats. Alex. J. Vet. Sci., 11: 95-99.
32. Hussain, H.E.M.A., 2002. Hypoglycemic, hypolipidemic and antioxidant properties of combination of curcumin from curcuma longa Linn, and partially purified product from Abroma augusta, Linn, in streptozotocin induced diabetes. Indian J. Clin. Biochem., 17: 33-43.
33. Ibraheim, Z.Z., 2002. Effect of Mgella sativa seeds and total oil on some blood parameters in female doxorubicin volunteers. Saudi Pharm. J., 10: 54-59.
34. Jaffar, A.R., J. Babb and A. Movahed. 2004. Optimal management of hyperlipidemia in primary prevention of cardiovascular disease. Int. J. Cardiol, 97: 355-366.
35. Kim, M. and Y. Kim, 2010. Hypocholesterolemic effects of curcumin via up-regulation of cholesterol 7 a-hydroxylase in rats fed a high fat diet. Nutr. Res. Pract, 4: 191-195.
36. Kocyigit, Y., Y. Atamer and E. Uysal, 2009. The effect of dietary supplementation of Mgella sativa L. on serum hpid profile in rats. Saudi Med. J., 30: 893-896.
37. Mahfouz, M., M. El-Dakhakhny, A. Gemel and H. Moussa, 1962. Choleretic action of Nigella sativa seed oil. Egypt. Pharm. Bull, 44: 225-229.
38. Mehta, A., G. Kaur and M. Chintamanem, 2012. Piperine and quercetin enhances antioxidant and hepatoprotective effect of curcumin in paracetamol induced oxidative stress. Int. J. Pharmacol., 8: 101-107.
39. Nader, M.A., D.S. El-Agamy and G.M. Suddek, 2010. Protective effects of propolis and thymoquinone on development of atherosclerosis in cholesterol-fed rabbits. Arch. Pharm. Res., 33: 637-643.
40. Nasir, Z. andM.A. Grashorn, 2010. Effects of Echinacea purpurea and Mgella sativa supplementation on broiler performance, carcass and meat quality. J. Anim. Feed Sci., 19: 94-104.
41. Nouzarian, R., S.A. Tabeidian, M. Toghyani, G. Ghalamkari and M. Toghyani, 2011. Effect of turmeric powder on performance, carcass traits, humoral immune responses and serum metabolites in broiler chickens. J. Anim. Feed Sci., 20: 389-400.
42. Pal, S., T. Choudhuri, S. Chattopadhyay, A., Bhattacharya, G.K. Datta, T. Das and G. Sa, 2001. Mechanisms of curcumin induced apoptosis of Ehrlicli s ascites carcinoma cells. Biochem. Biophys. Res. Commun., 288: 658-665.
43. Pourghassem-Gargari, B., V. Ebrahimzadeh-Attary, M. Rafraf and A. Gorbani, 2009. Effect of dietary supplementation with Nigella sativa L. on serum lipid profile, lipid peroxidation and antioxidant defense system in hyperlipidemic rabbits. J. Med. Plants Res., 3: 815-821.
44. Rathee, P.S., S.H. Mishra and R. Kaushal, 1982. Antimicrobial activity of essential oil, fixed oil and unsaponifiable matter of Mgella sativa L. Indian J. Pharm. Sci., 44: 8-10.
45. Reddy, A.C.P. andB.R. Lokesh, 1994. Alterations in lipid peroxidation in rat liver by dietary n-3 fatty acids: Modulation of anti-oxidant enzymes by curcumin, eugenol and vitamin E. J. Nutr. Biochem., 5: 181 -188.
46. Reinhold R.R., 1953. Determination of serum albumin. Clin. Chem.,21: 1370-1372.
47. Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol., 28: 56-63.
48. Salama, A.F. and S.M. El-Bahr, 2007. Effect of curcumin on cadmium-induced oxidative testicular damage in rats. J. Med. Res. Inst, 28: 167-173.
49. Shewita, R.S. and A.E. Taha, 2011. Effect of dietary supplementation of different levels of black seed (Mgella sativa L.) on growth performance, immunological, hematological and carcass parameters of broiler chicks. World Acad. Sci. Eng. Technol., 77: 788-794.
50. Sivabalan, S. and C.V. Anuradha, 2010. A comparative study on the antioxidant and glucose-lowering effects of curcumin and bisdemethoxycurcumin analog through in vitro assays. Int. J. Pharmacol., 6: 664-669.
51. Soliman, G.Z.A., 2005. Effect of curcumin, mixture of curcumin and piperine and curcum (Turmeric) on lipid profile of normal and hyperlipidemic rats. Egypt. J. Hosp. Med, 21: 145-161.
52. Srinivasan, K., 2005. Role of spices beyond food flavoring: Nutraceuticals with multiple health effects. FoodRev.Int, 21: 167-188.
53. Sugiharto, I, E. Widiastuti andN.S. Prabowo, 2011. Effect of turmeric on blood parameters, feed efficiency and abdominal fat content in broilers. J. Indonesian Trop. Anim. Agric, 36: 21-26.
54. Tabacco, A., F. Meiathini, E. Moda and P. Tarli, 1979. Simplified enzymic/colorimetric serum urea nitrogen determination. Clin. Chem., 25: 336-337.
55. Tasawar, Z.,Z. Siraj, N., Ahmad and M.H. Lashari, 2011. The effects oiNigella sativa (Kalonji) on lipid profile in patients with stable coronary artery disease in Multan, Pakistan. Pak. J. Nutr., 10: 162-167.
56. Tayyab, M., 1995. Effect of consumption of dietary elaesis guineensis (Palm kernel) seeds on serum lipid profile in albino rats. PhD. Thesis, Department of Pathology, Postgraduate Medical Institute, Lahore, Pakistan.
57. Tousson, E., M., El-moghazy and E. El-Atrsh, 2011. The possible effect of diets containing Nigella sativa and Thymus vulgaris on blood parameters and some organs structure in rabbit. Toxicol. Ind. Health, 27: 107-116.
58. Weggemans, R.M. and E.A. Trautwein, 2003. Relation between soyassociated lsoflavones and LDL and HDL cholesterol concentrations in humans: A meta analyses. Eur. J. Clin. Nutr, 57: 940-946.
59. Zak, B., RC. Dickenman, E.G. White, H. Burnett and P.J. Cherney, 1954. Rapid estimation of free and total cholesterol. Am. J. Clin. Pathol., 24: 1307-1315.
60. Zaoui, A., Y. Cherrah, K. Alaoui, N. Mahassine, H. Amarouch and M. Hassar, 2002. Effects oiNigella sativa fixed oil on blood homeostasis in rat. J. Ethnopharmacol., 79: 23-26.