Evaluation of cell-mediated immune responses against porcine circovirus type 2 (PCV2) Cap and Rep proteins after vaccination with a commercial PCV2 sub-unit vaccine

Veterinary Immunology and Immunopathology

Volumn 150, Issue 1-2, 2012, Pages 128-132

  Fort, Maria ^a,c   Sibila, Marina ^a   Nofrarías, Miquel ^a   Pérez Martín, Eva ^a,d   Olvera, Alex ^a,e   Mateu, Enric ^a,b   Segalés, Joaquim ^a,b  

^a CENTRE DE RECERCA EN SANITAT ANIMAL CRESA   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c PFIZER INC   (United States)

^d PLUM ISLAND ANIMAL DISEASE CENTER   (United States)

^e HOSPITAL UNIVERSITARI GERMANS TRIAS I PUJOL   (Spain)

Author keywords

Capsid protein; Cellular immunity; Humoral immunity; Porcine circovirus type 2 (PCV2); Replicase protein; Vaccine

Indexed keywords

CAP PROTEIN; GAMMA INTERFERON; HELICASE; PHOSPHATE BUFFERED SALINE; PORCINE CIRCOVIRUS TYPE 2A SUBUNIT VACCINE; SUBUNIT VACCINE; UNCLASSIFIED DRUG; VIRUS ANTIBODY; VIRUS PROTEIN;

ANIMAL EXPERIMENT; ANTIBODY TITER; ARTICLE; CELLULAR IMMUNITY; CONTROLLED STUDY; IMMUNOSTIMULATION; NONHUMAN; PROVOCATION TEST; SWINE; VACCINATION; VIREMIA;

ANIMALS; ANTIBODIES, VIRAL; CHI-SQUARE DISTRIBUTION; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; IMMUNITY, CELLULAR; INTERFERON-GAMMA; SWINE; SWINE DISEASES; VACCINES, SUBUNIT; VIRAL VACCINES; VIREMIA;

ANIMALIA; MIRIDAE; PORCINE CIRCOVIRUS 2; SUIDAE;

EID: 84867334554     PISSN: 01652427     EISSN: 18732534     Source Type: Journal    
DOI: 10.1016/j.vetimm.2012.09.001     Document Type: Article

References (18)

1. Fenaux M., Opriessnig T., Halbur P.G., Elvinger F., Meng X.J. A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J. Virol. 2004, 78:6297-6303.
2. Fort M., Olvera A., Sibila M., Segalés J., Mateu E. Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet. Microbiol. 2007, 125:244-255.
3. Fort M., Sibila M., Allepuz A., Mateu E., Roerink F., Segalés J. Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins. Vaccine 2008, 26:1063-1071.
4. Fort M., Fernandes L.T., Nofrarías M., Díaz I., Sibila M., Pujols J., et al. Development of cell-mediated immunity to porcine circovirus type 2 (PCV2) in caesarean-derived, colostrum-deprived piglets. Vet. Immunol. Immunopathol. 2009, 129:101-107.
5. Fort M., Sibila M., Pérez-Martín E., Nofrarías M., Mateu E., Segalés J. One dose of a porcine circovirus 2 (PCV2) sub-unit vaccine administered to 3-week-old conventional piglets elicits cell-mediated immunity and significantly reduces PCV2 viremia in an experimental model. Vaccine 2009, 27:4031-4037.
6. Fort M., Sibila M., Nofrarías M., Pérez-Martín E., Olvera A., Mateu E., Segalés J. Porcine circovirus type 2 (PCV2) Cap and Rep proteins are involved in the development of cell-mediated immunity upon PCV2 infection. Vet. Immunol. Immunopathol. 2010, 137:226-234.
7. Fraile L., Grau-Roma L., Sarasola P., Sinovas N., Nofrarías M., López-Jimenez R., López-Soria S., Sibila M., Segalés J. Inactivated PCV2 one shot vaccine applied in 3-week-old piglets: improvement of production parameters and interaction with maternally derived immunity. Vaccine 2012, 2:1986-1992.
8. Kekarainen T., McCullough K., Fort M., Fossum C., Segalés J., Allan G.M. Immune responses and vaccine-induced immunity against Porcine circovirus type 2. Vet. Immunol. Immunopathol. 2010, 136:185-193.
9. Kristensen C.S., Baadsgaard N.P., Toft N. A meta-analysis comparing the effect of PCV2 vaccines on average daily weight gain and mortality rate in pigs from weaning to slaughter. Prev. Vet. Med. 2011, 98:250-258.
10. Mankertz A., Caliskan R., Hattermann K., Hillenbrand B., Kurzendoerfer P., Mueller B., et al. Molecular biology of Porcine circovirus: analyses of gene expression and viral replication. Vet. Microbiol. 2004, 98:81-88.
11. Martelli P., Ferrari L., Morganti M., De Angelis E., Bonilauri P., Guazzetti S., et al. One dose of a porcine circovirus 2 subunit vaccine induces humoral and cell-mediated immunity and protects against porcine circovirus-associated disease under field conditions. Vet. Microbiol. 2011, 149:339-351.
12. Olvera A., Sibila M., Calsamiglia M., Segalés J., Domingo M. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J. Virol. Methods 2004, 117:75-80.
13. Opriessnig T., Patterson A.R., Elsener J., Meng X.J., Halbur P.G. Influence of maternal antibodies on efficacy of porcine circovirus type 2 (PCV2) vaccination to protect pigs from experimental infection with PCV2. Clin. Vaccine Immunol. 2008, 15:397-401.
14. Opriessnig T., Patterson A.R., Madson D.M., Pal N., Halbur P.G. Comparison of efficacy of commercial one dose and two dose PCV2 vaccines using a mixed PRRSV-PCV2-SIV clinical infection model 2-3-months post vaccination. Vaccine 2009, 27:1002-1007.
15. Pérez-Martín E., Grau-Roma L., Argilaguet J.M., Nofrarías M., Escribano J.M., Gomez-Sebastian S., et al. Development of two Trichoplusia ni larvae-derived ELISAs for the detection of antibodies against replicase and capsid proteins of porcine circovirus type 2 in domestic pigs. J. Virol. Methods 2008, 154(1-2):167-174.
16. Pérez-Martín E., Gómez-Sebastián S., Argilaguet J.M., Sibila M., Fort M., Nofrarías M., et al. Immunity conferred by an experimental vaccine based on the recombinant PCV2 Cap protein expressed in Trichoplusia ni-larvae. Vaccine 2010, 28:2340-2349.
17. Rosell C., Segalés J., Plana-Duran J., Balasch M., Rodríguez-Arrioja G.M., Kennedy S., et al. Pathological, immunohistochemical, and in situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J. Comp. Pathol. 1999, 120:59-78.
18. Steiner E., Balmelli C., Gerber H., Summerfield A., McCullough K. Cellular adaptive immune response against porcine circovirus type 2 in subclinically infected pigs. BMC Vet. Res. 2009, 22:5-45.