Evaluation of multi-well microelectrode arrays for neurotoxicity screening using a chemical training set

NeuroToxicology

Volumn 33, Issue 5, 2012, Pages 1048-1057

  McConnell, Emma R ^a   McClain, Maxine A ^a,c   Ross, James ^a   LeFew, William R ^b   Shafer, Timothy J ^b  

^a AXION BIOSYSTEMS   (United States)

^b U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^c Seahorse Bioscience Inc   (United States)

Author keywords

Cortical culture; Network function; Neurotoxicity; Screening, Microelectrode array; Training set

Indexed keywords

AMOXICILLIN; BICUCULLINE; BIFENTHRIN; CARBARIL; CYCLONITE; CYFLUTHRIN; DELTAMETHRIN; DIAZEPAM; DOMOIC ACID; FIPRONIL; FLUOXETINE; GLUTAMIC ACID; GLYPHOSATE; IMIDACLOPRID; KETAMINE; LEAD; LINDANE; METHYLMERCURY; MUSCIMOL; NICOTINE; PARACETAMOL; PARAQUAT; PERMETHRIN; SACCHARIN; SALICYLIC ACID; SORBITOL; TRIMETHYLTIN; VALPROIC ACID; VERAPAMIL;

ANIMAL CELL; ARTICLE; BRAIN CELL; CONTROLLED STUDY; DRUG SCREENING; IN VIVO STUDY; MICROELECTRODE ARRAY; NERVE CELL CULTURE; NERVE CELL NETWORK; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; RAT; SENSITIVITY AND SPECIFICITY; TOXICITY TESTING;

ACTION POTENTIALS; ANIMALS; ANIMALS, NEWBORN; CELLS, CULTURED; CEREBRAL CORTEX; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; MICROELECTRODES; NERVE NET; NEURONS; NEUROTRANSMITTER AGENTS; POISONS; RATS; RATS, LONG-EVANS; TETRODOTOXIN; TOXICITY TESTS;

EID: 84867233159     PISSN: 0161813X     EISSN: 18729711     Source Type: Journal    
DOI: 10.1016/j.neuro.2012.05.001     Document Type: Article

References (46)

1. Alkondon M., Albuquerque E.X. Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. I. Pharmacological and functional evidence for distinct structural subtypes. J Pharmacol Exp Ther 1993, 265(3):1455-1473.
2. Andersen M.E., Krewski D. Toxicity testing in the 21st century; bringing the vision to life. Toxicol Sci 2009, 107:324-330.
3. Bal-Price A.K., Suñol C., Weiss D.G., van Vliet E., Westerink R.H., Costa L.G. Application of in vitro neurotoxicity testing for regulatory purposes: symposium III summary and research needs. Neurotoxicology 2008, 29:520-531.
4. Biffi E., Ghezzi D., Pedrocchi A., Ferrigno G. Development and Validation of a spike detection and classification algorithm aimed at implementation on hardware devices. Comput Intell Neurosci 2010, 2010:1-15.
5. Boekelheide K., Campion S.N. Toxicity testing in the 21st century: using the new toxicity testing paradigm to create a taxonomy of adverse effects. Toxicol Sci 2010, 114:20-24.
6. Breier J., Radio N., Mundy W.R., Shafer T.J. Development of a high-throughput assay for assessing anti-proliferative compounds using human neuroprogenitor cells. Toxicol Sci 2008, 105:119-133.
7. Brown N.E., Kass R.E., Mitra P.P. Multiple neural spike train data analysis: state of the art and future challenges. Nat Neurosci 2004, 7:456461.
8. Cao Z., LePage K.T., Frederick M.O., Nicolaou K.C., Murray T.F. Involvement of caspase C activation in azaspiracit-induced neurotoxicity in neocortical neurons. Toxicol Sci 2010, 114:323-334.
9. Cao Z., Shafer T.J., Murray T.F. Mechanisms of pyrethroid insecticide-induced stimulation of calcium influx into neocortical neurons in vitro. J Pharmacol Exp Ther 2011, 336:197-205.
10. Cao Z., Shafer T.J., Crofton K.M., Gennings C., Murray T.F. Additivity of pyrethroid actions on sodium influx in cerebrocortical neurons in primary culture. Environ Health Perspect 2011, 9(9):1239-1246.
11. Castle N., Printzenhoff D., Zellmer S., Antonio B., Wickenden A., Silvia C. Sodium channel inhibitor drug discover using automated high throughput electrophysiology platforms. Comb Chem High Throughput Screen 2009, 12:107-122.
12. Crofton K., Mundy W., Lein P., Bal-Price A., Coecke S., Seiler A., et al. Developmental neurotoxicity testing: recommendations for developing alternative methods for the screening and prioritization of chemicals. Altern Anim Exp 2010, 28(1):9-15.
13. Crofton K.M., Mundy W.R., Lein P.J., Bal-Price A., Coecke S., Seiler A.E., Knaut H., Buzanska L., Goldberg A. Developmental neurotoxicity testing: recommendations for developing alternative methods for the screening and prioritization of chemicals. ALTEX 2011, 28:9-15.
14. Defranchi E., Novellino A., Whelan M., Vogel S., Ramirez T., van Ravenzwaay B., et al. Feasibility assessment of mirco-electrode chip assay as a method of detecting neurotoxicity in vitro. Front Neuroeng 2011, 4(6):1-12.
15. Dunlop J., Bowlby M., Peri R., Vasilyev D., Arias R. High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology. Nat Rev 2008.
16. Frega M., Pasquale V., Tedesco M., Marcoli M., Contestabile A., Nanni M., et al. Cortical cultures coupled to micro-electrode arrays: a novel approach to perform in vitro excitotoxicity testing. Neurotoxicol Teratol 2011, (August). [Epub ahead of print].
17. Gopal K. Neurotoxic effects of mercury on auditory cortex networks growing on microelectrode arrays: a preliminary analysis. Neurotoxicol Teratol 2003, 25:69-76.
18. Gramowski A., Schiffmann D., Gross G.W. Quantification of acute neurotoxic effects of trimethyltin using neuronal networks cultures on microelectrode arrays. Neurotoxicology 2000, 21:331-342.
19. Gross G.W., Rhoades B.K., Azzazy H.M.E., Wu M-C. The use of neuronal networks on multielectrode arrays as biosensors. Biosens Bioelectron 1995, 10:553-567.
20. Gross G.W., Harsch A., Rhoades B.K., Göpel W. Oder, drug and toxin analysis with neuronal networks in vitro: extracellular array recording of network responses. Biosens Bioelectron 1997, 12(5):373-393.
21. Hogberg H.T., Sobanski T., Novellino A., Whelan M., Weiss D.G., Bal-Price A.K. Application of micro-electrode arrays (MEAs) as an emerging technology for developmental neurotoxicity: evaluation of domoic acid-induced effects in primary cultures of rat cortical neurons. Neurotoxicology 2011, 32:158-168.
22. Joesch C., Guevarra E., Parel S.P., Bergner A., Zbinden P., Konrad D., et al. Use of FLIPR membrane potential dyes for validation of high-throughput screening with the FLIPR and microARCS technologies: identification of ion channel modulators acting on the GABA(A) receptor. J Biomol Screen 2008, 13:218-228.
23. Johnstone A.F., Gross G.W., Weiss D.G., Schroeder O.H., Gramowski A., Shafer T.J. Microelectrode arrays: a physiologically based neurotoxicity testing platform for the 21st century. NeuroToxicology 2010, 31(4):331-350.
24. Keefer E.W., Norton S.J., Boyle N.A.J., Talesa V., Gross G.W. Acute toxicity screening of novel AChE inhibitors using neuronal networks on microelectrode arrays. NeuroToxicology 2001, 22:3-12.
25. Levkovitz Y., Gil-Ad I., Zeldich E., Dayag M., Weizman A. Differential induction of apoptosis by antidepressants in glioma and neuroblastoma cell lines. J Mol Neurosci 2005, 27:29-42.
26. Liu K., Southall N., Titus S.A., Inglese J., Eskay R.L., Shinn P., et al. A multiplex calcium assay for identification of GPCR agonists and antagonists. Assay Drug Dev Technol 2010, 8(3):367-379.
27. Losa S.M., Johnstone A.F.M., Shafer T.J. Relative potencies of type I and type II pyrethroids for inhibition of spontaneous firing in neuronal networks. Toxicol CD Soc Toxicol 2009, 108(1). Abstract # 2126.
28. Meyer D.A., Carter J.M., Johnstone A.F.M., Shafer T.J. Pyrethroid modulation of spontaneous neuronal excitability and neurotransmission in hippocampal neurons in culture. Neurotoxicology 2008, 29:213-225.
29. Nagata K., Song J.H., Shono T., Narahashi T. Modulation of the neuronal nicotinic acetylcholine receptor-channel by the nitromethylene heterocycle imidacloprid. J Pharmacol Exp Ther 1998, 285:731-738.
30. Novellino A., Scelfo B., Palosaari T., Price A., Sobanski T., Shafer T., et al. Development of micro-electrode array based tests for neurotoxicity: assessment of interlaboratory reproducibility with neuroactive chemicals. Front Neuroeng 2011, 4(4):1-14.
31. NRC Toxicity testing in the twenty-first century: a vision and a strategy 2007, The National Academies Press, Washington, DC.
32. Pereira A., Cao Z., Murray T.F., Gerwick W.H., Hoiamide A. A sodium channel activator of unusual architecture from a sonsortium of two Papua New Guinea cyanobacteria. Chem Biol 2009, 16:893-906.
33. Pope C.N. Organophosphorus pesticides: do they all have the same mechanism of toxicity?. Toxicol Appl Pharmacol 1999, 158:186-196.
34. Radio N., Breier J., Shafer T.J., Mundy W.R. Development of a high-throughput assay for assessing neurite outgrowth. Toxicol Sci 2008, 105:106-118.
35. Radio N.M., Mundy W.R. Developmental neurotoxicity testing in vitro: models for assessing chemical effects on neurite outgrowth. NeuroToxicology 2008, 29:361-376.
36. Robinette B.L., Harrill J.A., Mundy W.R., Shafer T.J. In vitro assessment of developmental neurotoxicity: use of microelectrode arrays to measure functional changes in neuronal network ontogeny. Front Neuroeng 2011, 4:1.
37. Schroeder O.H.-U., Gramowski A., Jügelt K., Teichmann C., Weiss D. Spike train data analysis of substance-specific network activity: application to functional screening in preclinical development. Proceedings of the 6th international meeting on substrate-integrated microelectrodes 2008.
38. Shafer T.J., Rijal S.O., Gross G.W. Complete inhibition of spontaneous activity in neuronal networks in vitro by deltamethrin and permethrin. Neurotoxicology 2008, 29:203-212.
39. Slotkin T.A. Cholinergic systems in brain development and disruption by neurotoxicants: nicotine, environmental tobacco smoke, organophosphates. Toxicol Appl Pharmacol 2004, 198:132-151.
40. Soderlund D.M., Clark J.M., Sheets L.P., Mullin L.S., Piccirillo V.J., Sargent D., et al. Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment. Toxicology 2002, 171(1):3-59.
41. Sokal D.M., Mason R., Parker T.L. Multi-neuronal recordings reveal a differential effect of thapsigargin on bicuculline- or gabazine-induced epileptiform excitability in rat hippocampal neuronal networks. Neuropharmacology 2000, 39:2408-2417.
42. Tateno T., Jimbo Y., Robinson H.P.C. Spatio-temporal cholinergic modulation in cultured networks of rat cortical neurons: spontaneous activity. Neuroscience 2005, 134:425-437.
43. Tomizawa M., Casida J.E. Neonicotinoid insecticide toxicology: mechanisms of selective action. Annu Rev Pharmacol Toxciol 2005, 45:247-268.
44. van Vliet E., Stoppini L., Balestrino M., Eskes C., Griesinger C., Sobanski T., Whelen M., Hartung T., Coecke S. Electrophysiological recording of re-aggregating brain cell cultures on multi-electrode arrays to detect acute neurotoxic effects. Neurotoxicology 2007, 28:1136-1146.
45. Williams L.R., Johnstone A.F.M., Bannon D.I., Shafer T.J. RDX binds to the convulsant site of the GABAA receptor and increases spontaneous firing rates of cortical neurons. Toxicol CD Soc Toxicol 2010, 114(1). Abstract # 216.
46. Wolansky M.J., Gennings C., Crofton K.M. Relative potencies for acute effects of pyrethroids on motor function in rats. Toxicol Sci 2006, 89:271-277.