Use of FLIPR membrane potential dyes for validation of high-throughput screening with the FLIPR and μARCS technologies: Identification of ion channel modulators acting on the GABAA receptor

Journal of Biomolecular Screening

Volumn 13, Issue 3, 2008, Pages 218-228

  Joesch, Christoph ^a   Guevarra, Emelie ^a   Parel, Serge P ^a   Bergner, Andreas ^a   Zbinden, Peter ^a   Konrad, Daniel ^b   Albrecht, Hugo ^a,c  

^a BioFocus DPI   (United Kingdom)

^b bSys Biological Monitoring Systems GmbH   (Switzerland)

^c UNIVERSITY OF APPLIED SCIENCES AND ARTS NORTHWESTERN SWITZERLAND   (Switzerland)

Author keywords

ARCS; FLIPR; FMP Dye; GABAA receptor; Ion channel screening

Indexed keywords

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID A RECEPTOR; 4 AMINOBUTYRIC ACID A RECEPTOR ALPHA1; 4 AMINOBUTYRIC ACID A RECEPTOR BETA2; 4 AMINOBUTYRIC ACID A RECEPTOR GAMMA2; 4 AMINOBUTYRIC ACID A RECEPTOR STIMULATING AGENT; DIAZEPAM; DYE; ION CHANNEL; UNCLASSIFIED DRUG;

ALLOSTERISM; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; ELECTROPHYSIOLOGY; FLUOROMETRIC IMAGING PLATE READER; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MEMBRANE POTENTIAL; MICROARRAYED COMPOUND SCREENING; PRIORITY JOURNAL; RECEPTOR GENE; TECHNOLOGY; VALIDATION PROCESS;

ANIMALS; BIOLOGICAL ASSAY; CELL LINE; COLORING AGENTS; DIAZEPAM; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; ELECTROPHYSIOLOGY; FLUORESCENCE; GAMMA-AMINOBUTYRIC ACID; HUMANS; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT MODULATORS; PERTUSSIS TOXIN; RATS; RECEPTORS, GABA-A; REPRODUCIBILITY OF RESULTS;

RATTUS;

EID: 40049107993     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057108315036     Document Type: Article

References (21)