Evaluation of two methods for quantification of hsp70 mRNA from the waterborne pathogen Cryptosporidium parvum by reverse transcription real-time PCR in environmental samples

Water Research

Volumn 43, Issue 10, 2009, Pages 2669-2678

  Garcés Sanchez, Gabriela ^a   Wilderer, Peter A ^a   Munch, Jean Charles ^c   Horn, Harald ^a   Lebuhn, Michael ^a,b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b Institute for Agricultural Engineering and Animal Husbandry   (Germany)

^c INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

Cryptosporidium parvum; Hsp70 mRNA; Manure; Nucleic acid extraction; Oocyst lysis; Real time quantitative PCR

Indexed keywords

EXTRACTION; FERTILIZERS; MANURES; TRANSCRIPTION;

CRYPTOSPORIDIUM PARVUM; GUANIDINIUM; HSP70 MRNA; ISOTHIOCYANATES; NUCLEIC ACIDS EXTRACTIONS; OOCYST LYSE; OOCYSTS; REAL-TIME PCR; REAL-TIME QUANTITATIVE PCR; REVERSE TRANSCRIPTION;

POLYMERASE CHAIN REACTION;

MESSENGER RNA;

BACTERIUM; CHLOROFORM; DETECTION METHOD; GENE EXPRESSION; INJURY; LYSIS; MANURE; OPTIMIZATION; PATHOGEN; PHENOL; POLYMERASE CHAIN REACTION; PROTIST; QUANTITATIVE ANALYSIS; RNA; VIABILITY;

ARTICLE; CRYPTOSPORIDIUM PARVUM; EVALUATION; GENE; HSP70 GENE; INTERMETHOD COMPARISON; MANURE; NONHUMAN; OOCYST; PRIORITY JOURNAL; PURIFICATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WATER SAMPLING;

CRYPTOSPORIDIUM PARVUM;

EID: 65549093106     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.watres.2009.03.019     Document Type: Article

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